Overview
Uridine phosphorylase (UPase) is an essential enzyme in the pyrimidine salvage pathway, responsible for the reversible conversion of uridine to uracil and ribose-1-phosphate. This enzyme is widely distributed in microorganisms, plants, and animals, with significant variations in molecular weight and kinetic properties among different sources. In pharmaceutical applications, UPase has gained attention for its role in activating prodrugs like 5-fluorouridine in cancer chemotherapy. The enzyme's specificity and catalytic efficiency make it valuable for biochemical research, particularly in studying nucleotide metabolism and designing enzyme inhibitors for therapeutic purposes.
Physical and Chemical Properties
Uridine phosphorylase typically appears as a white lyophilized powder or clear solution when reconstituted. The enzyme demonstrates optimal activity at neutral to slightly alkaline pH (7.0-8.0) and maintains stability at physiological temperatures (up to 37°C). Its molecular weight ranges between 32-35 kDa depending on the source, with microbial-derived enzymes often being smaller than mammalian variants. The enzyme requires inorganic phosphate for its phosphorolysis reaction and is inhibited by some nucleotide analogs, which is particularly relevant for drug development applications.
Main Applications
The primary application of uridine phosphorylase lies in pharmaceutical research, where it serves as a target for anticancer and antiviral drug development. The enzyme's ability to activate certain nucleoside analogs makes it crucial for prodrug conversion strategies in chemotherapy. In industrial biotechnology, UPase is used for the enzymatic synthesis of nucleoside analogs and for studying nucleotide metabolism pathways. Research laboratories employ this enzyme for biochemical assays and metabolic studies, particularly in investigations of pyrimidine metabolism disorders.
Safety and Storage
Uridine phosphorylase should be handled with standard laboratory precautions, including gloves and protective eyewear. While not classified as highly hazardous, direct contact with skin or mucous membranes should be avoided. For storage, the lyophilized form is stable at -20°C for extended periods, while solutions should be aliquoted to avoid repeated freeze-thaw cycles that can diminish activity. Shipping typically occurs on dry ice for long-distance transport, with cold chain maintenance being critical for preserving enzymatic activity.
B2B Procurement Guide
When procuring uridine phosphorylase, buyers should prioritize specifications including enzymatic activity (typically 1-10 units/mg protein), purity level (≥90% by SDS-PAGE), and absence of contaminating nucleases. Microbial-derived enzymes often offer cost advantages over mammalian sources for many applications. Lead times for specialty preparations can range from 2-6 weeks, with bulk quantities sometimes requiring custom production. Quality documentation should include certificates of analysis detailing specific activity, endotoxin levels (for therapeutic applications), and stability data. Consider vendor reputation in supplying enzymes for pharmaceutical versus research use when making procurement decisions.
Related Manufacturers
- 主营：科研试剂盒、elisa试剂盒、细胞、酶免试剂盒、生物试剂、进口ELISA试剂盒、PCR试剂盒、生化测试盒
- 主营：科研试剂盒、elisa试剂盒、生化试剂盒、酶免试剂盒、细胞、生物试剂、进口ELISA试剂盒、PCR试剂盒、生化测试盒
- 主营：酶澳生物、elisa试剂盒
- 主营：酶联免疫试剂盒、Elisa试剂盒、检测试剂盒
- 主营：Elisa试剂盒、生化试剂盒、蛋白试剂盒、细胞试剂盒
- 主营：ATCC细胞系、原代细胞、原代永生化
- 主营：检测试剂盒、elisa试剂盒
- 主营：Elisa试剂盒、生化试剂盒、Pcr试剂盒、酶联免疫法、细胞、抗体
- 主营：D-荧光素钾、D-荧光素钠、缓冲液、脲酶Urease、黄嘌呤氧化酶、胰α淀粉酶、β-葡萄糖苷酶、辣根过氧化物酶、胰蛋白酶抑制剂、乙酰胆碱酯酶、L-乳酸脱氢酶、过氧化氢酶、葡萄糖氧化酶、裂解液、刀豆凝集素ConA、植物检测试剂盒、tRNA转运核糖核酸、肝素钠、福林酚、三羟甲基氨基甲烷、二硫苏糖醇DTT、苏木色精、伊红Y(曙红)、乙基紫、土壤检测试剂盒
- 主营：氯霉素、糖蛋白、试剂盒、磷酸酶、磷脂酶、凝血酶、连接酶、分泌酶、纤溶酶、脂蛋白、胆固醇、钙蛋白、粘液素、调蛋白、人嗅素、饥饿素、抑制因、凝集素、白介素、胰岛素、阻滞剂、结合盒、防御素、干扰素、板层素
- 主营：Elisa试剂盒、人elisa试剂盒、大鼠elisa试剂盒、酶联免疫试剂盒、小鼠elisa试剂盒、植物elisa试剂盒
- 主营：ringers液、聚山梨酯、葡萄糖溶液、检测试剂盒、氯化锂溶液、dna提取试剂盒、染色液、色素
- 主营：elisa试剂盒、白细胞介素试剂盒、肿瘤坏死因子试剂盒、酶联免疫试剂盒、植物检测试剂盒
- 主营：elisa试剂盒
- 主营：酶联免疫检测试剂盒、ELISA试剂盒、PCR检测试剂盒、抗体