Overview
Pancreatic Digest of Casein Medium is a hydrolysate of milk protein casein, produced through enzymatic digestion using pancreatic enzymes. It serves as a fundamental nutrient base in microbiology due to its balanced composition of amino acids and small peptides. The medium supports the growth of fastidious microorganisms and is a staple in laboratories for both research and industrial applications. Unlike synthetic media, it offers natural growth factors that enhance bacterial recovery rates. Its consistency and batch-to-batch reproducibility make it a preferred choice for standardized testing protocols, such as those in pharmacopoeias (e.g., USP, EP).
Physical and Chemical Properties
The medium appears as a hygroscopic powder with a slight characteristic odor. It dissolves readily in water to form a clear, amber solution with a pH of approximately 6.5–7.5 after sterilization. Its composition includes 14–18% total nitrogen, with tryptophan (a key indicator for indole tests) present at 1.2–2.5%. Thermal stability is moderate; autoclaving at 121°C for 15 minutes is standard. The product may darken after prolonged heat exposure but retains its nutritive properties. Trace metals (e.g., calcium, magnesium) are naturally present from the casein source.
Main Applications
Primarily used in clinical diagnostics to isolate pathogens like Salmonella and Staphylococcus. It’s also integral to vaccine manufacturing as a substrate for bacterial toxin production (e.g., tetanus toxin). In food safety, it aids in detecting contaminants per ISO 6579 and FDA BAM methods. Industrial applications include fermentation media for antibiotics (e.g., penicillin) and enzyme production. Its low carbohydrate content minimizes acid formation, making it suitable for pH-sensitive processes. Specialty grades are available for cell culture and virology.
Safety and Storage
While non-hazardous, prolonged dust exposure may cause respiratory irritation. Store in original packaging with desiccants to prevent clumping. Opened containers should be resealed tightly and used within 6 months to avoid moisture absorption and microbial contamination. For disposal, incineration or landfill is acceptable per local regulations. Sterilize waste media via autoclaving before discarding to inactivate potential biological agents. Regulatory compliance (e.g., ISO 13485) is critical for medical-grade batches.
B2B Procurement Guide
Procure from suppliers with ISO 9001 certification and documented traceability. Request certificates of analysis (CoA) for critical parameters like growth performance (e.g., USP <61> specifications). Bulk buyers (100+ kg) should negotiate batch testing and shelf-life guarantees. For international shipments, ensure temperature-controlled logistics to prevent condensation. Consider pre-sterilized ready-to-use formats to reduce lab preparation time. Alternatives like soy peptone may be evaluated for allergen-free requirements.
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