Overview
Trichoderma reesei is a mesophilic fungus first isolated during WWII for its ability to degrade cotton textiles. It has since become a workhorse in industrial biotechnology due to its exceptional capacity to secrete cellulolytic enzymes. The strain’s genome was fully sequenced in 2008, enabling targeted genetic modifications to enhance enzyme yields. As a saprophytic organism, T. reesei naturally thrives on decaying plant material. Industrial strains like RUT-C30 are derived from repeated mutagenesis, achieving enzyme titers exceeding 100 g/L. Its GRAS (Generally Recognized As Safe) status by regulatory agencies facilitates use in food and feed applications.
Physical and Chemical Properties
T. reesei exhibits typical fungal morphology with branching hyphae and conidiophores producing green spores. Colonies grow rapidly (2–3 days) on PDA medium at 25–30°C, with optimal enzyme production at pH 4–5 and 28°C. The secreted enzyme cocktail includes endoglucanases, exoglucanases, β-glucosidases, and xylanases. The fungus metabolizes cellulose to glucose via synergistic enzyme action, with reported cellulase activities reaching 100–150 FPU/mL in optimized fermentations. Its enzymes remain stable at temperatures up to 50–60°C, making them suitable for industrial processes requiring thermotolerance.
Main Applications
In biofuel production, T. reesei enzymes convert agricultural residues (e.g., corn stover, bagasse) into fermentable sugars for ethanol. The textile industry employs its cellulases for biostoning denim and biopolishing fabrics. Additional uses include animal feed digestion enhancers and juice clarification in food processing. Recent advances focus on engineered strains for consolidated bioprocessing (CBP), combining enzyme production and fermentation. The fungus also shows promise in mycoremediation of lignocellulosic waste, reducing environmental pollution from agro-industrial byproducts.
Safety and Storage
While classified as non-pathogenic, T. reesei spores may cause respiratory irritation in sensitive individuals. Industrial facilities should implement HEPA filtration and personal protective equipment (PPE) during large-scale cultivation. Lyophilized cultures retain viability for 5+ years at -80°C with proper cryoprotectants. Enzyme preparations typically contain preservatives like glycerol or sodium benzoate. Shipping requires adherence to IATA regulations for biological substances (Category B, UN3373). Inactivation can be achieved by autoclaving (121°C, 15 min) or treatment with 1% sodium hypochlorite.
B2B Procurement Guide
Buyers should specify required enzyme activities (e.g., FPU for cellulases, XU for xylanases) and purity levels (e.g., protease-free for textile use). Fermentation scale (lab-grade vs. industrial batches) significantly impacts pricing—bulk orders of 1,000+ liters often receive 20–30% discounts. Key suppliers include Novozymes, DuPont, and AB Enzymes. For custom strains, lead times average 8–12 weeks for genetic modification and fermentation optimization. Quality control should verify absence of bacterial contamination (e.g., via PCR testing) and enzyme thermostability through SDS-PAGE analysis.
Related Manufacturers
- 主营：架桥剂、YW100 锂萃取剂、端羧基液体丁腈橡胶、里氏木霉、10-羟基癸酸、苯乙烯磷酸、肉豆蔻酸镁、硬脂酸钴、全氟三丁胺、乙烯基磷酸二乙酯、磷酸锰铁锂、β-磷酸三钙、十一烯酸、钨酸铝、1-乙炔基环已醇、间二异丙苯、锂硅合金、溴甲酚绿、生物活性玻璃、溴代海因树脂颗粒、红铝、龙涎酮、原丁酸三甲酯
- 主营：枯草芽孢杆菌、聚谷氨酸、微生物菌剂、哈茨木霉、淡紫紫孢菌
- 主营：化工中间体、有机中间体、香精香料、添加剂、表面活性剂、天然/合成树脂、分析试剂、日化原料、催化剂、高分子材料
- 主营：氯菊酯、转移剂、聚酯树脂、二甲氧基、驱蚊原液、食品香料、缩水甘油酯、蚊香增效剂、丙烯酸缩水、环氧稀释剂、硅烷偶联剂、超疏水材料、对甲氧基苯酚、对羟基苯甲醚、乙烯基双封头、苯基缩水甘油醚
- 主营：磷酸三钙、钡玻璃粉、锶玻璃粉、生物活性玻璃、虫胶粉/精制紫胶、全氟三丁胺、卡波姆树脂、月桂酰赖氨酸、木瓜蛋白酶、低温玻璃粉、磺化栲胶、聚酰亚胺pi树脂微粉、八甘醇、羟基磷灰石、高取代羟丙基纤维素、异戊橡胶、杜仲胶、二水硫酸钙、壳聚糖、氢化卵磷脂、辛烯基琥珀酸淀粉钠、花椒麻素、甲氧基聚乙二醇丙烯酸、维生素A棕榈酸酯、水解胶原蛋白锌
- 主营：生物活性玻璃、全氟三丁胺、花椒麻素、里氏木霉、二羟基丙酮、2-十一酮、光引发剂、乙酸龙脑酯、羟基磷灰石
- 主营：硫化剂、阻燃剂、甘氨酸、卡必醇、碳酸镁、芹菜酮、视黄醇、壬二炔、赖氨酸、藏花醛、甘羟铝、化合物、浮选剂、稀释剂、酸性红、羟乙基、芳叶油、庚二酸、庚二酮、十八烷、碳酸锌、三甲酚、甲酸钾、乙酸锌、碳酸锶
- 主营：苯基脲、二异戊基硫醚、己基硫醚、辛基硫醚、壳聚糖季铵盐
- 主营：ELISA试剂盒、PCR试剂盒、生化试剂盒、里氏木霉、脂蛋白、关基因、亲环素、钾通道、列抗体、ntp溶液、蛋白酶、脑蛋白、病毒抗、蛋白质、239抗体、dtt溶液、科研试剂
- 主营：igg抗体、试剂盒、淋巴瘤、绿木霉、测试盒、菌种、细胞、elisa、PCR、荧光PCR、大肠杆菌、标准品、瘤细胞、癌细胞、抗体、核酸染料、elisa试剂盒、荧光PCR试剂盒、核酸检测试剂盒、牙龈卟啉单胞菌、链球菌、检测卡、酶联免疫分析试剂盒