Overview
Trehalase is a glycoside hydrolase enzyme that specifically breaks down trehalose into two glucose molecules. It is found in microorganisms, plants, and animals, including humans, where it aids in carbohydrate metabolism. The enzyme is critical in industries requiring trehalose conversion, such as food manufacturing and biotechnology. Trehalase is categorized into two types: acid trehalase (lysosomal) and neutral trehalase (cytosolic). Its activity is often measured in units per milligram, with industrial-grade enzymes optimized for stability under processing conditions.
Physical and Chemical Properties
Trehalase exhibits optimal activity at a slightly acidic pH (5.0–6.5) and temperatures around 37°C, though thermostable variants exist for industrial use. The enzyme is typically supplied as a lyophilized powder to prolong shelf life, with solubility limited to aqueous solutions. Its molecular weight ranges between 70–75 kDa, depending on the source (e.g., microbial vs. mammalian). The enzyme is inactivated by heavy metals and sulfhydryl reagents, requiring careful handling during formulation.
Main Applications
In the food industry, trehalase is used to hydrolyze trehalose in honey, mushrooms, and processed foods to enhance sweetness or fermentation efficiency. It also serves as a diagnostic tool for trehalose intolerance research. Pharmaceutical applications include enzyme replacement therapy for rare metabolic disorders like congenital trehalase deficiency. In biotechnology, it aids in trehalose-related pathway studies and biofuel production from trehalose-rich biomass.
Safety and Storage
While trehalase is non-toxic, airborne powder may irritate respiratory tracts. Use gloves and masks during handling. Store lyophilized enzyme at -20°C; reconstituted solutions are stable for 1–2 weeks at 4°C if sterile-filtered. Avoid contamination with proteases or metal ions, which degrade activity. Long-term storage requires desiccated conditions to prevent moisture absorption.
B2B Procurement Guide
When sourcing trehalase, prioritize suppliers providing certificates of analysis (CoA) detailing activity (U/mg), purity (SDS-PAGE), and endotoxin levels. Industrial buyers should confirm scalability and batch consistency. For research-grade enzymes, opt for recombinant versions with higher specificity. Prices vary by purity (e.g., ≥90% for pharmaceuticals vs. ≥70% for food use). Bulk orders (1 kg+) may reduce costs by 20–30%.
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