Overview
Human Total Protein represents the complete proteome extracted from human biological samples, including plasma, tissues, or cell cultures. It serves as a critical reference material in life science research, providing researchers with a standardized protein mixture for comparative studies. The preparation typically involves advanced fractionation techniques to preserve protein integrity while removing contaminants. Quality control measures ensure batch-to-batch consistency, making it valuable for proteomics studies, biomarker discovery, and assay development.
Physical and Chemical Properties
As a complex mixture, Human Total Protein exhibits varying physical properties depending on its source and preparation method. The lyophilized form appears as a white to off-white powder, while solutions are typically clear to slightly opalescent. Protein concentrations in commercial preparations range from 1-10 mg/mL, with pH adjusted to physiological ranges (7.2-7.6). The composition includes both high-abundance proteins like albumin and immunoglobulins, as well as low-abundance signaling proteins and enzymes, mirroring natural human proteome distributions.
Main Applications
In research laboratories, Human Total Protein serves as a positive control in Western blotting, ELISA, and mass spectrometry experiments. Diagnostic manufacturers use it to calibrate instruments and validate test kits for human protein detection. The pharmaceutical industry employs these protein mixtures in drug target identification and therapeutic antibody development. Recent applications include proteome profiling for personalized medicine approaches and comparative studies between healthy and diseased states.
Safety and Storage
Proper handling requires biosafety level 2 precautions due to potential exposure to human-derived materials. Always use personal protective equipment and work in designated biological safety cabinets when preparing solutions. For optimal stability, store lyophilized preparations at -20°C in desiccated conditions. Reconstituted solutions should be aliquoted to avoid repeated freeze-thaw cycles, with most formulations remaining stable for 1-2 weeks at 4°C or several months at -80°C.
B2B Procurement Guide
When sourcing Human Total Protein, prioritize vendors with certified biological sourcing and rigorous quality control. Key specifications to verify include protein concentration (BCA/Lowry assay verified), endotoxin levels (<1 EU/μg), and absence of protease activity. For specialized applications, consider requesting source information (e.g., pooled plasma vs. specific tissue) and accompanying characterization data such as 2D gel electrophoresis patterns or mass spectrometry profiles. Bulk purchases (100mg+) typically offer 20-30% cost savings compared to research-grade quantities.
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