Overview
Sulfo-Cyanine5-Chitosan is a functionalized biopolymer combining chitosan's structural properties with the near-infrared fluorescence of Cy5 dye. The sulfonation enhances water solubility, making it ideal for biological applications. This conjugate is synthesized through covalent bonding of sulfo-Cy5 NHS ester to chitosan's primary amines. The degree of labeling (DOL) typically ranges from 1-5%, balancing fluorescence intensity with minimal structural disruption to chitosan.
Physical and Chemical Properties
The compound exhibits strong absorption at ~650 nm and emission at ~670 nm, allowing deep-tissue imaging with minimal background interference. Sulfonation prevents dye aggregation and improves biocompatibility. Unlike non-sulfonated Cy5-chitosan, this derivative maintains stability in physiological pH ranges (4-9). Its fluorescence intensity is pH-dependent but remains functional across most biological conditions. The chitosan backbone provides mucoadhesive properties and biodegradability.
Main Applications
In biomedical research, it serves as a tracer for drug delivery systems, enabling real-time tracking of chitosan-based nanoparticles in vivo. Its NIR fluorescence is valuable for whole-animal imaging studies. The conjugate is also used for cell surface labeling, particularly in immune cell tracking experiments. In diagnostics, it functions as a contrast agent for fluorescence-guided surgeries and endoscopic procedures.
Safety and Storage
While generally low-toxicity, powder form may cause respiratory irritation. Always handle in fume hoods with nitrile gloves. Aqueous solutions are stable for 1-2 weeks at 4°C when protected from light. For long-term storage, lyophilized powder should be kept in amber vials with desiccant at -20°C. Avoid repeated freeze-thaw cycles of solutions, which may quench fluorescence.
B2B Procurement Guide
When sourcing, specify: 1) Chitosan molecular weight (commonly 10-50 kDa), 2) Degree of deacetylation (>85% preferred), 3) Cy5 labeling ratio, and 4) endotoxin levels (<0.1 EU/mg for in vivo use). Bulk orders (100g+) may qualify for 15-30% discounts. Request batch-specific fluorescence spectra and HPLC purity certificates. For GMP-grade material, expect 2-3x price premiums and longer lead times.
Related Manufacturers
- 主营：反应活、hydrazide、修饰剂、磺化、催化剂、糖蛋白、连接子、激动剂、聚合物、活性脂、胆固醇、钆特酸、催化性、dna标记、络合剂、荧光试剂、雨蛙素、乙二胺、ped结构、衍生物、化合物、链接剂、反应物、生物素
- 主营：磺化、纳米颗粒、脂质体
- 主营：壳聚糖季铵盐、植提专用酶、防腐剂系列、增稠剂系列
- 主营：注射级海藻糖、苯甲醇、三乙醇胺、壳聚糖、甜菊糖苷、注射级甘露醇、牛磺酸、醋酸钠、木糖醇、黄原胶、甘露醇、大豆油、薄荷脑、注射级木糖醇、二甲硅油、卡波姆、枸橼酸、磷酸氢二钠、磷酸氢二钾、明胶、十六醇、十八醇、聚维酮k30、注射级甘油
- 主营：阻燃剂、拉那白、牛儿酮、羧甲基壳聚糖、海藻糖、热敏蓝、萘磺酸、氯菊酯、硫酸锌、催化剂、杨梅酯、檀香醚、聚醚胺、吸收剂、脂檀油、苍术素、羟钴胺、转移剂、溶剂绿、麝香酮、王浆酸、芬多精、纤维素、新戊酯、王冠醚、遮甜粉
- 主营：苦参碱、氧化苦参碱、七叶皂苷钠、壳聚糖、七叶皂苷、黑米花色苷、鬼臼树脂、鬼臼毒素、黑米花青素、龙胆苦苷、青蒿琥酯、青蒿素、吴茱萸碱、吴茱萸次碱、博落回总碱、鱼藤酮、二苯乙烯苷、丝肽粉、人参皂甙
- 主营：营养强化剂、抗氧化剂、甜味剂、壳聚糖季铵盐、食品级增稠剂、食用色素、酶制剂、粉末香精、酸度调节剂、食品乳化剂、着色剂、抗结剂、增味剂
- 主营：杀菌剂、椰子油、印染剂、壳聚糖、阻燃剂、油酸锌、润湿剂、硫酸铯、催化剂、粘合剂、乳化剂、草酸锰、硫酸锂、石英砂、绝缘油、茶皂素、防腐剂、醋酸镁、锂电池、碘化锂、培养基、蒸馏水、硬脂酸、塑化剂、草酸钾、磷酸铁
- 主营：低聚果糖、低聚木糖、甘露寡糖、羧甲基壳聚糖厂家、菊粉、麦芽糖醇、木糖醇、纽甜、乳糖、乳糖醇、三氯蔗糖、聚葡萄糖、索马甜、瓜尔豆胶、果胶、海藻酸丙二醇酯、海藻酸钠、黄原胶、卡拉胶、可得然胶、大豆多糖、魔芋粉、塔拉胶、琼脂、沙蒿籽胶、明胶
- 主营：葵二酸、乙二醇、防锈剂、1303-86-2、防冻液、润滑剂、荧光绿、酒石酸、增稠剂、钛白粉、增白剂、k12粉状、丙三醇、建筑防水、去除异味、蒸气湿地、三乙醇胺、氨基硅油、厂家直发、水杨酸粉、铁黑颜料、异十三醇、十八酸铁、水产养殖、松香
- 主营：甘油、丙二醇、聚山梨酯80、药用级壳聚糖、二甲硅油、二甲基亚砜、苯甲酸苄酯、苯甲醇、泊洛沙姆、聚丙烯酸钠、海藻糖、大豆磷脂、黄原胶、羧甲基纤维素钠、依地酸二钠、海藻酸钠、醋酸氯己定、葡萄糖酸氯己定、聚乙烯醇、聚维酮k30、交联聚维酮、交联羧甲基纤维素钠、RH40
- 主营：全氟聚醚二醇、全氟三丁胺、聚硅氮烷、羧甲基壳聚糖、特种硅油、特种硅树脂
- 主营：羧甲基壳聚糖、全氟三丁胺、木质素、吉氏色素
- 主营：消泡剂、杀菌剂、润湿剂、壳聚糖、乳化剂、交联单体、增稠剂、vae乳液、分散剂、附着力、爽滑剂、增塑剂、上光油、流平剂
- 主营：己二酸二酰肼、丙酸氯倍原料、亚乙基硫脲、2-甲基四氢噻吩-3-酮、3-甲基-2-丁硫醇、2-辛二醇、三氟甲基磺酸铋、醋酸钯