Overview
Specific demethylases are enzymes that catalyze the removal of methyl groups from nucleic acids (DNA/RNA) or proteins, thereby modulating gene expression and cellular function. They are pivotal in epigenetic regulation, with notable examples including TET (Ten-Eleven Translocation) enzymes for DNA demethylation and FTO (Fat Mass and Obesity-associated protein) for RNA demethylation. These enzymes are highly specific to their substrates and often require cofactors like α-ketoglutarate and iron. Research into demethylases has expanded rapidly due to their implications in diseases such as cancer and neurological disorders. Biotech and pharmaceutical industries leverage these enzymes for drug discovery and therapeutic applications, particularly in developing epigenetic modulators.
Physical and Chemical Properties
Demethylases are typically large proteins with molecular weights varying by family (e.g., TET enzymes ~200 kDa). They function optimally at physiological pH (7.0-7.5) and temperatures (37°C), though stability depends on storage conditions. Lyophilized forms retain activity longer but require reconstitution in specific buffers. Enzymatic activity is often measured via assays detecting conversion of methylated substrates (e.g., 5mC to 5hmC for TET enzymes). Sensitivity to oxygen and metal ions (e.g., Fe²⁺) necessitates controlled handling. Commercial preparations may include stabilizers like glycerol or BSA to prolong shelf life.
Main Applications
In research, demethylases are used to study epigenetic mechanisms, including gene silencing and cellular differentiation. They are critical tools in CRISPR/dCas9-based epigenome editing. Pharmaceutical applications focus on targeting aberrant methylation in cancers (e.g., inhibiting DNMT or activating TET enzymes). Diagnostically, demethylase activity serves as a biomarker for certain cancers. Emerging therapies exploit these enzymes to reverse pathological methylation patterns, with clinical trials underway for hematological malignancies. Agricultural biotech also explores demethylases to enhance crop traits via epigenetic modulation.
Safety and Storage
While non-toxic, demethylases require handling with gloves and sterile techniques to prevent contamination. Avoid repeated freeze-thaw cycles; aliquot liquid forms for single-use. Store lyophilized enzymes at -20°C and reconstituted solutions at -80°C. Disposal should follow biohazard protocols if used with human/animal samples. Activity loss may occur if exposed to light or oxidizing agents. Always verify storage buffers (e.g., DTT for sulfhydryl protection) and cofactor requirements before use.
B2B Procurement Guide
When procuring demethylases, prioritize suppliers with documented enzyme activity (units/µg) and purity (≥90% by SDS-PAGE). Request lot-specific data, including kinetic parameters (Km/Vmax) and substrate specificity. Bulk orders may require custom formulations or stability testing. For therapeutic applications, ensure compliance with cGMP standards. Compare lead times and scalability, as some enzymes are produced via recombinant expression with limited batch sizes. Negotiate pricing for long-term contracts, especially for high-throughput screening projects.
Related Manufacturers
- 主营：ELISA试剂盒、PCR试剂盒、生化试剂盒、蛋白、抗体、细胞、检测试剂盒
- 主营：α2巨球、素d合成、纤连蛋白、酶cγ链、金属肽酶、蛋白激酶、抗组蛋白、肌醇抗体、蛋白样环、敏感蛋白、牛骨钙素、蛋白重链、乳球蛋白、钩端螺旋、高血糖素、钙调蛋白、小鼠α1抗、ELISA试剂盒、pcr检测试剂、生化检测试剂
- 主营：ELISA试剂盒、抗体
- 主营：试剂盒、ELISA试剂盒、科研ELISA试剂盒、PCR试剂盒、生化试剂盒
- 主营：酶联免疫、elisa试剂盒
- 主营：ELISA试剂盒、PCR试剂盒、生化试剂盒、蛋白酶、脂蛋白、关基因、亲环素、钾通道、列抗体、ntp溶液、脑蛋白、病毒抗、蛋白质、239抗体、dtt溶液、科研试剂
- 主营：ATCC细胞系、原代细胞、原代永生化
- 主营：酶联免疫试剂盒、试剂盒
- 主营：elisa试剂盒、生化试剂盒、抗体、细胞、pcr试剂盒
- 主营：检测试剂盒、elisa试剂盒
- 主营：Elisa试剂盒、人elisa试剂盒、大鼠elisa试剂盒、酶联免疫试剂盒、小鼠elisa试剂盒、植物elisa试剂盒
- 主营：试剂盒、辅激活因子、elisa试剂盒、蛋白、多肽、金标试剂盒、PCR试剂盒、抗生素、氨基酸、血清、抗体
- 主营：ELISA试剂盒、高灵敏度试剂盒、试剂盒厂家、人ELISA试剂盒、小鼠ELISA试剂盒、大鼠ELISA试剂盒、仓鼠ELISA试剂盒、豚鼠ELISA试剂盒、鸡ELISA试剂盒、鸭ELISA试剂盒、牛ELISA试剂盒、羊ELISA试剂盒、马ELISA试剂盒、猴ELISA试剂盒、猪ELISA试剂盒、犬ELISA试剂盒、兔ELISA试剂盒、鱼ELISA试剂盒、植物ELISA试剂盒、土壤ELISA试剂盒、定性ELISA试剂盒、肿瘤标志物检测ELISA试剂盒、特种蛋白检测ELISA试剂盒、传染病检测ELISA试剂盒
- 主营：酶联免疫法、elisa试剂盒
- 主营：[]