Overview
Soluble E-selectin is a cleaved form of the transmembrane protein E-selectin (CD62E), released during endothelial cell activation. As a key mediator of leukocyte adhesion, it serves as a biomarker for inflammatory conditions like sepsis, atherosclerosis, and autoimmune diseases. Unlike its membrane-bound counterpart, sE-selectin circulates in blood plasma, making it accessible for diagnostic testing. Commercial preparations are typically recombinant proteins produced in mammalian expression systems, ensuring proper glycosylation for biological activity.
Physical and Chemical Properties
As a glycoprotein, sE-selectin's molecular weight varies slightly (110-120 kDa) due to glycosylation differences. The protein contains multiple domains, including a lectin domain that specifically recognizes carbohydrate ligands like sialyl-Lewis X. The lyophilized form is stable for years at -20°C but requires reconstitution in sterile buffers (pH 7.2-7.4). In solution, it remains stable for weeks at 4°C, though repeated freeze-thaw cycles should be avoided. Activity is typically measured via ELISA or cell adhesion inhibition assays.
Main Applications
In clinical research, sE-selectin quantifies endothelial dysfunction in conditions like diabetes mellitus and preeclampsia. Pharmaceutical companies use it to screen anti-inflammatory drugs targeting selectin-mediated adhesion. Cancer researchers study its role in metastasis, as tumor cells exploit selectins to extravasate. Additionally, it's incorporated into in vitro models of vascular inflammation, often alongside ICAM-1 and VCAM-1 for comprehensive leukocyte migration studies.
Safety and Storage
While not classified as hazardous, standard laboratory precautions apply. Use gloves and avoid aerosol generation when handling lyophilized powder. For long-term storage, aliquot reconstituted solutions to minimize degradation. Lyophilized vials should include desiccants to prevent moisture absorption. Avoid solutions with visible particulates or discoloration, as these indicate protein degradation. For critical experiments, verify activity via positive control samples.
B2B Procurement Guide
When sourcing sE-selectin, prioritize vendors providing: 1) Certificate of Analysis with purity data, 2) Species specificity (human/mouse/rat), and 3) application-specific validation (e.g., ELISA standard curves). Bulk purchasers should negotiate batch consistency guarantees, as glycosylation patterns may vary between production runs. For specialized needs (e.g., fluorescent labeling), inquire about custom modification services. Lead times for GMP-grade material often exceed 8 weeks.
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