Overview
The Six-Stage Andersen Microbial Sampler is a sophisticated air monitoring device designed for microbiological assessment in controlled environments. Based on the Andersen impaction principle developed in the 1950s, this instrument physically separates airborne particles into six distinct size ranges through successive impaction stages. Each stage contains agar plates to culture collected microorganisms, allowing for both quantitative and qualitative analysis of bioaerosols. The sampler's tiered design corresponds to human respiratory deposition patterns, making it particularly valuable for infection control studies. Modern versions incorporate precision-machined components to maintain the critical 28.3 L/min (1 CFM) airflow rate specified in regulatory guidelines. This device has become an industry standard for pharmaceutical cleanroom monitoring and hospital IAQ assessments.
Structure and Working Principle
Constructed with 304-grade stainless steel impaction plates and an anodized aluminum outer casing, the sampler features six stacked perforated stages with progressively smaller hole diameters (from 0.25mm to 0.34mm). A vacuum pump draws air through these stages at a controlled rate, causing particles to impact onto separate agar-filled Petri dishes based on their aerodynamic diameter. The operational principle relies on inertial impaction - as air changes direction abruptly at each stage, particles with sufficient momentum detach from the airstream and deposit on the collection surface. Stage 1 collects the largest particles (≥7μm), while Stage 6 captures the smallest (≤0.65μm). This stratification enables correlation between particle size and potential respiratory penetration depth, crucial for risk assessment in sterile manufacturing environments.
Key Features
This microbial sampler stands out for its precision engineering and regulatory compliance. The calibrated nozzle array ensures uniform flow distribution across all stages, while the stainless steel construction permits repeated autoclaving (typically at 121°C for 15-20 minutes) without deformation. Many models include a built-in flow meter and timer for accurate sample volume calculation. Modern variants may incorporate features like wireless data logging, HEPA-filtered exhaust systems, and compatibility with various culture media. The device's 400-hole design per stage provides statistically valid sampling according to ISO 14698-1 standards. Importantly, the sampler's performance characteristics are well-documented in peer-reviewed studies, allowing for method validation in GMP environments.
Application Areas
Pharmaceutical cleanrooms constitute the primary application for these samplers, where they're used for routine environmental monitoring (EM) programs to demonstrate aseptic processing compliance. In vaccine production facilities, they help identify potential contamination sources by characterizing microbial populations in different airborne size fractions. Hospital infection control teams employ these devices to assess operating theater air quality and investigate nosocomial outbreaks. Additional applications include university research labs studying aerobiology, food processing plants monitoring hygiene conditions, and containment facilities verifying biosafety measures. The data obtained supports regulatory submissions to agencies like FDA, EMA, and WHO.
Maintenance and Precautions
Proper maintenance begins with post-use decontamination - all stainless steel components require autoclaving, while electronic parts need wipe-down with 70% isopropanol. Regular verification includes airflow calibration (typically quarterly) using a primary standard flow meter and inspection of nozzle plates for scratches that could affect collection efficiency. Critical precautions involve avoiding sampler operation in environments with explosive gases or conductive dusts. Users must ensure agar plates are properly secured to prevent media displacement during sampling. When moving between different cleanliness zones, the sampler should undergo full decontamination to prevent cross-contamination. Storage should be in a clean, dry environment with protective caps on all orifices.
B2B Procurement Guide
When procuring these specialized samplers, buyers should prioritize suppliers with ISO 17025-accredited calibration services. Key specifications to verify include: stage cut-point accuracy (±5% of nominal values), flow rate stability (±5% over 4 hours), and material certificates for stainless steel components. Many GMP facilities require full IQ/OQ documentation packages. Consider total cost of ownership - some premium models offer longer service intervals and better part availability. For international buyers, confirm the device meets both ISO 14698 and USP <1116> requirements. Lead times for calibrated units typically range 4-8 weeks. Bulk purchases (5+ units) often attract 10-15% discounts from manufacturers. Some suppliers provide rental options for short-term validation projects.
Related Manufacturers
- 主营：[]
- 主营：[]
- 主营：食品安全检测仪、农药残留检测仪、水质检测仪、新型空气浮游菌采样器、ATP荧光检测仪、微生物检测仪、食用油检测仪、农药残留快速检测仪、农残检测仪、农残速测仪、食品检测仪、食品安全速测仪、高智能食品安全检测仪、土壤养分检测仪、食品快检设备、土壤氮磷钾检测仪、食品实验室设备、食品安全检测仪器、茶叶农药残留检测仪、手持atp荧光检测仪、兽药残留检测仪、土壤肥料养分检测仪、多功能食品安全检测仪、卡尔费休水分仪、尘埃粒子计数器、食品安全快检设备
- 主营：[]
- 主营：浮游菌采样器、尘埃粒子计数器
- 主营：空气流量、检测设备、性检测仪、浮游菌、流量检测、高精度差压、泄漏测试仪、密封测试仪、气密检测仪、防水测试仪、流量测试仪、性测试设备、气溶胶发生器、密封性测试仪
- 主营：食品安全检测仪、农药残留检测仪、土壤养分检测仪、新型空气浮游菌采样器、ATP荧光检测仪、兽药残留检测仪、食品检测仪、水质检测仪、食品安全快检设备、食品添加剂检测仪器、食品微生物检测仪、农残快速检测设备、土壤重金属检测仪、多参数水质快速检测仪、大肠杆菌检测仪、亚硝酸盐检测仪器、病害肉检测仪、水产品安全检测仪、卡尔费休水分测定仪、鸡蛋品质检测仪
- 主营：全自动凯氏定氮仪、红外测油仪、台式多参数水质检测仪、浮游菌、重金属检测仪、BOD测定仪、食品安全测定仪、农药残留检测仪、液液萃取仪、一体化万用蒸馏仪、电厂专用硅酸根检测仪、紫外测油仪、便携式多参数水质检测、磷酸根测定仪、粗纤维测定仪、测脂肪测定仪、全自动电位滴定仪、离子计、COD测定仪、余氯测定仪、紫外多参数检测仪、光度计、溶解氧仪、水质理化箱、综合重金属检测仪、滴定仪
- 主营：[]
- 主营：[]
- 主营：光谱仪、分析仪、监测仪、采样器、望远镜、蒸干仪、测定仪、铁谱仪、流量计、研磨机、消解器、铬酸盐、混匀仪、测试仪、露点仪、反应罐、锰酸盐、浊度仪、均质器、检测仪、蒸馏仪、浓缩仪、清洗机、残氧仪、测油仪
- 主营：风速计、测量仪、分析仪、采样器、噪声仪、测定仪、传感器、cod氨氮、体温仪、照度仪、溴离子、消解仪、体温枪、酸度计、探测器、红外线、测尘仪、干燥箱、检测仪、显微镜、测色仪、电导仪、水质仪、voc气体、报警器
- 主营：油气回收检测仪、便携式活性炭碘值检测、烟花爆竹探测器、空气微生物采样器、便携式明渠流量计、单人防护装备、个人剂量报警仪、氢火焰离子化检器、水质快检试剂包、手持热电风速仪、水样采样设备、便携式噪声检测仪、多参数气体检测仪、热成像泄漏检测仪、土壤重金属检测仪、移动源执法监测设备、水质指纹预警溯源仪、便携式烟气分析仪、便携式恶臭检测仪、便携式油烟检测仪、林格曼黑度检测仪、消耗臭氧层物质检测仪、应急防护包、辐射防护包、个人防护包、油气回收多参数检测仪
- 主营：检测仪、voc测试仪、油烟测试仪、大气采样器、水质采样器、自动监测仪、油烟分析仪、油烟监测仪、气体检测仪、水质分析仪、烟气分析仪、明渠流量计、环境检测仪
- 主营：便携式明渠流量计、水质五参数检测仪、便携式水质分析仪、水质采样器、流量检测、水质多参数检测仪、水质监测、顶空分析仪、污水流量计、在线分析仪、自动监测仪、表面活性剂、明渠流量计、在线监测仪、流速测定仪、环境氡测量仪、铁离子监测仪、重金属监测仪、黑球温度指数仪、电子皂膜流量计、COD氨氮检测仪、风速仪
- 主营：浮游菌采样器、粒子计数器、洁净度检测仪
- 主营：黑度仪、场强仪、检测仪、自动采样器、水质采样器、微生物采样器、气溶胶采样器、风速仪、检测箱、外置泵、电磁兼容、流速监测仪、紫外辐照仪、水质监测仪、黑烟识别器、黑球指数仪、水质速测箱、风速测量仪、甲醛测试仪、明渠流量计、孔口流量计、微电脑控制器、电磁场测量仪、手持式抓拍仪、恒温恒湿设备
- 主营：分析仪、防护服、监测仪、采样器、压力计、报警仪、粉尘仪、测量仪、水分仪、汞蒸汽、黑度仪、测温仪、测定仪、传感器、速测仪、流量计、采泥器、风量罩、辐射仪、机器人、测试仪、噪音计、消解仪、防护包、检测箱
- 主营：核酸采样小屋、核酸采样工作站、气体检测仪、水质采样器、大气采样器、便携式自动水质采样器、水质检测仪、激光粉尘仪、恒温恒湿称重系统、便携式明渠流量计、voc气体检测仪、实验室仪器
- 主营：在线水质采样器、明渠流量计
- 主营：气体分析仪、日本理研、日本新宇宙、浮游菌、水质检测仪、英国离子、霍尼韦尔
- 主营：低浓度烟尘测试仪、热湿法紫外烟气分析仪、手持气体检测仪、环境空气综合采样器、水质自动采样器、大流量采样器、浓缩微生物采样器、深水洗井采样器、颗粒物采样器、生物毒性检测仪、大流量空气微生物采样、β射线烟尘直读检测仪、WBGT指数仪、碳排放监测仪、红外CO分析仪、便携式水样抽滤器、烟气综合分析仪、红外CO2分析仪、恶臭气体检测仪、水质抽滤仪、红外烟气综合分析仪、紫外臭氧测定仪、便携式明渠流量计
- 主营：分析仪、监测站、监测仪、采样器、报警仪、抽滤器、粉尘仪、测量仪、加热器、地下水、测定仪、传感器、稀释剂、匀浆机、测速仪、消解器、浓度计、采集器、流量计、纯水机、测试仪、试验箱、噪音计、度监测、离心机
- 主营：梅思安、多组分、复合气体、粉尘采样器、环境检测仪、气体探测器、甲醛检测仪、烟气分析仪、便携式流速、气体检测仪、红外测温仪、非甲烷总烃、油气回收检测仪
- 主营：voc检测仪、恒湿称重系统、油烟监测仪、自动采样器、大气采样器、水质采样器、氟化物采样器、油烟检测仪、光散射、测定仪、cod消解、粉尘连续、采样测试仪、水质检测仪、烟气检测仪、在线分析仪、烟气含湿量、气体检测仪、烟气分析仪、回收检测仪、内置锂电池、自动分析仪、粉尘检测仪、激光粉尘仪、氧化碳分析仪
- 主营：在线水质、气体检测仪、核酸隔离箱、浮游菌、多功能声级计