Overview
Human Sirtuin 6 (SIRT6) is a member of the sirtuin family of proteins, which are NAD+-dependent enzymes involved in cellular regulation. SIRT6 is primarily known for its roles in DNA repair, metabolic homeostasis, and aging. It functions as both a deacetylase and a mono-ADP-ribosyltransferase, targeting histones and other proteins to modulate gene expression and stress responses. Research has highlighted SIRT6's importance in maintaining genomic stability and suppressing inflammation. Its overexpression has been linked to extended lifespan in animal models, making it a focal point for studies on aging and age-related diseases. SIRT6 is also implicated in cancer biology, where it can act as a tumor suppressor or promoter depending on the context.
Physical and Chemical Properties
SIRT6 is a protein with a molecular weight of approximately 39 kDa. It is typically supplied as a lyophilized powder or in liquid solution for research purposes. The enzyme requires NAD+ as a cofactor for its deacetylase and mono-ADP-ribosyltransferase activities, which are central to its biological functions. SIRT6 is soluble in aqueous buffers and is stable under recommended storage conditions (-20°C or -80°C). Its activity can be affected by pH, temperature, and the presence of inhibitors or activators. The protein's structure includes a catalytic domain that binds NAD+ and substrate proteins, enabling its regulatory functions.
Main Applications
SIRT6 is widely studied in biomedical research for its roles in aging, cancer, and metabolic diseases. In aging research, SIRT6 is investigated for its ability to enhance DNA repair and reduce oxidative stress, potentially delaying age-related decline. Cancer studies explore its dual role as a tumor suppressor and promoter, depending on the cellular context. In metabolic research, SIRT6 regulates glucose and lipid metabolism, making it a target for diabetes and obesity studies. Additionally, SIRT6's involvement in inflammation and immune responses has implications for autoimmune and inflammatory diseases. Therapeutic strategies targeting SIRT6 are under development, including small-molecule activators and inhibitors.
Safety and Storage
SIRT6 should be handled with standard laboratory precautions, including the use of gloves and protective equipment. The protein is sensitive to repeated freeze-thaw cycles, which can degrade its activity. For long-term storage, aliquots should be kept at -20°C or -80°C. Researchers should verify the purity and activity of SIRT6 before use, as these factors can significantly impact experimental results. Contaminants or inactive protein may lead to inconsistent data. Proper documentation of storage conditions and handling procedures is essential for reproducibility.
B2B Procurement Guide
When procuring SIRT6 for research or commercial use, consider the source (recombinant or native), purity, and activity levels. Recombinant SIRT6 is commonly produced in E. coli or mammalian cells and is preferred for consistency. Purity should be confirmed via SDS-PAGE or HPLC, and activity assays should be performed to ensure functionality. Prices vary depending on the supplier, quantity, and purity, with research-grade SIRT6 typically costing $200-$500 per mg. Bulk purchases may offer discounts. Reputable suppliers provide certificates of analysis (CoA) detailing purity, concentration, and activity. Compare vendors for reliability, customer support, and shipping conditions to ensure product integrity.
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