Overview
Sheep Apolipoprotein is a class of proteins synthesized in sheep liver and intestines, crucial for lipid transport in blood plasma. These proteins bind to lipids to form lipoproteins, facilitating cholesterol and triglyceride metabolism. Research on sheep-derived apolipoproteins provides insights into comparative lipid biology, given their structural similarities to human counterparts like ApoA-I and ApoE. In biomedical fields, sheep apolipoproteins are valued for their stability and compatibility with cross-species studies. They serve as models for atherosclerosis research and are used to develop diagnostic assays for lipid disorders. Commercial preparations typically isolate specific subtypes (e.g., ApoA-I) from sheep serum via ultracentrifugation and chromatography.
Physical and Chemical Properties
Sheep Apolipoproteins exhibit amphipathic structures, with hydrophobic domains for lipid interaction and hydrophilic surfaces for aqueous solubility. Their molecular weights vary by subtype—ApoA-I is approximately 28 kDa, while ApoB can exceed 500 kDa. These proteins are heat-labile, denaturing at temperatures above 60°C, and susceptible to proteolytic degradation if improperly stored. In solution, they form stable complexes with phospholipids, a property exploited in laboratory reconstitutions of high-density lipoprotein (HDL). Solubility depends on buffer conditions; neutral pH (7.0-7.4) and low ionic strength (e.g., 10 mM Tris) are optimal. Lyophilized powders retain activity for years at -20°C but require careful rehydration to avoid aggregation.
Main Applications
The primary use of Sheep Apolipoprotein lies in cardiovascular disease research, particularly studies mimicking human lipid metabolism. They are incorporated into synthetic HDL particles to investigate cholesterol efflux mechanisms or serve as antigens in ELISA kits for lipoprotein quantification. Diagnostic manufacturers utilize these proteins to calibrate instruments measuring lipid profiles. Veterinary laboratories also employ sheep-specific apolipoprotein assays to monitor metabolic health in livestock. Emerging applications include nanoparticle drug delivery systems, where apolipoproteins enhance targeting to liver cells.
Safety and Storage
While non-toxic, Sheep Apolipoprotein preparations may contain trace contaminants from serum extraction. Always handle with gloves and avoid inhalation of lyophilized powder. Storage at -20°C (lyophilized) or -80°C (solution) prevents degradation; repeated freeze-thaw cycles should be minimized. For long-term stability, aliquot solutions into single-use vials under inert gas (argon). Suppliers typically provide certificates of analysis detailing endotoxin levels (<1 EU/mg) and sterility. Dispose of waste following local biohazard regulations, even though the protein itself is not classified as hazardous.
B2B Procurement Guide
When procuring Sheep Apolipoprotein, prioritize suppliers specializing in animal serum proteins and verify batch-specific documentation. Key parameters include purity (≥95% by SDS-PAGE), residual moisture (<5% for lyophilized forms), and absence of hemoglobin contamination. Request samples for pilot testing if the protein will be used in sensitive assays. Bulk purchases (10+ mg) often reduce costs by 20-30%. Consider custom purification services if specific isoforms (e.g., ApoA-II) are required. Lead times vary; certified research-grade material typically ships within 2-4 weeks.
Related Manufacturers
- 主营：科研试剂盒、酶免试剂盒、elisa试剂盒、绵羊载脂蛋白B100、生化试剂盒、细胞、生物试剂、进口ELISA试剂盒、PCR试剂盒、生化测试盒
- 主营：科研试剂盒、酶免试剂盒、elisa试剂盒、绵羊载脂蛋白A1、细胞、生物试剂、进口ELISA试剂盒、PCR试剂盒、生化测试盒
- 主营：绵羊载脂蛋白B、elisa 试剂盒
- 主营：ELISA试剂盒、高灵敏度试剂盒、试剂盒厂家、人ELISA试剂盒、小鼠ELISA试剂盒、大鼠ELISA试剂盒、仓鼠ELISA试剂盒、豚鼠ELISA试剂盒、鸡ELISA试剂盒、鸭ELISA试剂盒、牛ELISA试剂盒、羊ELISA试剂盒、马ELISA试剂盒、猴ELISA试剂盒、猪ELISA试剂盒、犬ELISA试剂盒、兔ELISA试剂盒、鱼ELISA试剂盒、植物ELISA试剂盒、土壤ELISA试剂盒、定性ELISA试剂盒、肿瘤标志物检测ELISA试剂盒、特种蛋白检测ELISA试剂盒、传染病检测ELISA试剂盒
- 主营：elisa试剂盒、白细胞介素试剂盒、肿瘤坏死因子试剂盒、绵羊载脂蛋白B、植物检测试剂盒
- 主营：elisa试剂盒、生化试剂盒、pcr检测试剂盒、载脂蛋白A1、细胞
- 主营：氯霉素、糖蛋白、试剂盒、绵羊载脂蛋白B100、脂蛋白、胆固醇、钙蛋白、粘液素、调蛋白、磷酸酶、人嗅素、饥饿素、磷脂酶、抑制因、凝血酶、连接酶、凝集素、分泌酶、白介素、胰岛素、阻滞剂、结合盒、防御素、纤溶酶、干扰素、板层素
- 主营：ELISA试剂盒、抗体、细胞、载脂蛋白A1、PCR试剂盒
- 主营：小鼠载脂蛋白B100ELISAKit、ELISA试剂盒、PCR试剂盒、科研试剂
- 主营：科研试剂盒、酶免试剂盒、elisa试剂盒、细胞、生物试剂、进口ELISI试剂盒、PCR试剂盒
- 主营：ELISA试剂盒、PCR试剂盒、生化试剂盒、蛋白、抗体、细胞、检测试剂盒
- 主营：igg抗体、糖蛋白、试剂盒、脂蛋白、人瘦素、粗纤维、猪睾酮、烯二酮、nad激酶、测试盒、牛瘦素、牛胆酸、原果胶、磷酸酶、犬肾素、含水量、人蛋白、甜菜碱、igm抗体、dna抗体、磷脂酶、大鼠超、犬叶酸、人胆酸、样受体
- 主营：ELISA试剂盒、PCR试剂盒、生化试剂盒、APOL5载脂蛋白L5ELISAKit、脂蛋白、关基因、亲环素、钾通道、列抗体、ntp溶液、蛋白酶、脑蛋白、病毒抗、蛋白质、239抗体、dtt溶液、科研试剂
- 主营：载脂蛋白A2elisa试剂盒、试剂盒、人25羟基维生素、人低氧诱导因子
- 主营：ELISA试剂盒、PCR试剂盒、生化试剂盒、科研试剂