Overview
Serum-Free Medium (SFM) is a chemically defined alternative to traditional serum-containing media, engineered to eliminate variability and contamination risks associated with animal-derived components. It typically includes growth factors, hormones, and nutrients tailored to specific cell types, ensuring reproducible results in research and industrial applications. SFM is critical in biopharmaceutical manufacturing, where regulatory compliance and product consistency are paramount. Its adoption has grown due to ethical concerns over animal serum use and the demand for standardized processes. Leading suppliers offer formulations for adherent or suspension cultures, with customization options for unique cell lines or production needs.
Physical and Chemical Properties
SFM is a sterile, aqueous solution with a pH range of 7.2–7.4, optimized for cell viability. Its composition avoids undefined additives like fetal bovine serum (FBS), replacing them with recombinant proteins and synthetic substitutes. Key components often include insulin, transferrin, and trace elements such as selenium. The absence of serum reduces batch-to-batch variability and minimizes interference in downstream purification. Physical properties like osmolarity (280–320 mOsm/kg) and viscosity are tightly controlled to mimic physiological conditions. Shelf life varies but typically ranges from 6–12 months when stored properly.
Main Applications
SFM is indispensable in biotech and pharmaceutical industries for monoclonal antibody production, viral vaccine development, and cell therapy manufacturing. Its consistency supports regulatory filings by ensuring process transparency and reducing adventitious agent risks. In research, SFM enables studies of cell behavior without serum-induced artifacts, particularly in stem cell and cancer research. Specialty formulations exist for hybridoma, CHO, and Vero cells, among others. The shift to SFM in large-scale bioreactors has improved yields and reduced costs by simplifying purification workflows.
Safety and Storage
While SFM is non-hazardous, sterile techniques are essential to prevent microbial contamination. Pre-use filtration (0.22 µm) is recommended for non-sterilized batches. Gloves and lab coats should be worn during handling to maintain aseptic conditions. Storage at 2–8°C preserves stability, though some formulations tolerate brief room-temperature exposure. Avoid repeated freeze-thaw cycles, which may precipitate components. Dispose of expired media following local biohazard guidelines, even if chemically inert.
B2B Procurement Guide
When sourcing SFM, prioritize suppliers with ISO 13485 or GMP certification to ensure quality. Request certificates of analysis (CoA) for endotoxin levels (<1 EU/mL) and performance testing data. Bulk discounts are often available for volumes above 100 liters. Evaluate scalability: some media require adaptation periods for cells transitioning from serum. Pilot testing is advisable. Consider ready-to-use formats for convenience or dry powder variants to reduce shipping costs. Lead times can vary; secure supply agreements for critical production lines.
Related Manufacturers
- 主营：[]
- 主营：超净工作台、高压蒸汽灭菌器、生物安全柜、低温冰箱、二氧化碳培养箱、生化培养箱、除颤仪、经皮黄疸仪、婴儿培养箱、生化分析仪、安全柜检测过滤器更换、红外线灭菌器、移液器、医用离心机、智慧药房、包埋机、脱水机、酶联免疫分析仪、欧莱博液氮罐、欧莱博移液器、欧莱博纯水机、欧莱博天平、欧莱博通风柜、切片机、化学发光免疫分析仪
- 主营：一恒培养箱、生物安全柜、米欧实验室小仪器、无血清细胞冻存液、宝予德移液器、弗鲁克电动搅拌器、奥浦迈培养基
- 主营：试剂、耗材、仪器、Wako无血清细胞冻存液、转染试剂、实验耗材、origene蛋白、takara试剂、CST抗体、abcam抗体、permagen磁力、polyplus转染、AcceGen细胞、qiagen试剂盒、zymo试剂盒、goldbio荧光素、Toxin肠毒素、速溶颗粒、DMEM培养基、胎牛血清、alzet渗透泵、伊莱瑞特、millipore、EZB试剂盒、细胞核悬液制备试剂盒、解离试剂盒
- 主营：819刀片、PCR管、微孔板、无血清细胞冻存液、细胞板、蛋白酶、八联管、转印膜、病理刀片、免疫组化笔、细胞计数板、离心管、降温盒、移液槽、蛋白定量试剂盒、病理切片石蜡、PBS片剂、ECL发光液、二甲基亚砜、PCR封板膜、梯度降温冻存盒、PVDF转印膜、盒装吸头、内皮细胞培养基ECM、湿转印仪、牛血清白蛋白
- 主营：无血清细胞冻存液、生化试剂、化学试剂
- 主营：吩嗪、肌氨酸钠、偶氮二异丁脒盐酸盐、V50、H酸、DDS、甲基磺酸铋、二恶唑啉、1-苯基-5-巯基四氮唑、柠檬酸铋、赛克、双氟代碳酸乙烯酯
- 主营：染色液、感受态、滴定法、无酚红、试剂盒、鸡血清、保存液、赖氨酸、脯氨酸、抗凝牛、猫血清、苏木素、巴氏法、缬氨酸、乙酸锂、类器官、青霉素、苏氨酸、添加剂、色氨酸、酪氨酸、pbs干粉、噻唑蓝、硫酸镁、鲑鱼精
- 主营：生化试剂、蛋白与免疫、ELISA、生化试剂盒、染色试剂、微生物培养、磁珠、纳米材料、抗体
- 主营：涂料助剂、工业清洗剂、表面活性剂、无血清、合成材料助剂、合成香料、溶剂、香精和香料中间、合成材料中间体、选矿药剂、萃取剂
- 主营：间充质干细胞无血清培养基、新一代间充质干细胞化学成分限定培养基、微载体
- 主营：加帽酶、激活剂、甘氨酸、27无血清添加剂、igg抗体、稳定液、玻璃板、试剂盒、脂蛋白、超纯水、激动剂、胆固醇、盐酸盐、因质粒、扩容剂、组化笔、驴抗人、预制胶、驴抗兔、生物驴、草甘膦、重组酶、生物素、曲拉通、去除剂、封闭液
- 主营：染色液、乙氨酸、噻唑兰、无血清细胞、喷雾剂、封片剂、清除剂、试剂盒、研试剂、转膜液、dpph试剂、page凝胶、te缓冲液、pbs缓冲液、检测溶液、核酸染料、中性树胶、胰蛋白酶、纤维素酶、染色试剂、mes缓冲液、faa固定液、甲苯胺蓝、生化试剂、实验试剂、ctab抽提液
- 主营：蛋白酶、试剂盒、al缓冲液、无血清添加剂、pb缓冲液、eb缓冲液、一级代理、过滤漏斗、载物夹片、中国代理、尼龙过滤膜、核糖核酸酶、不锈钢过滤器、真空驱动装置、尾气检测滤膜、清洁度检测滤膜
- 主营：无血清细胞、实验试剂、标准溶液、显色试剂盒
- 主营：保护柱、泵样管、飞刃环、线圈盒、质谱仪、移液器、试剂盒、螺口盖、检测器、样品瓶、止回阀、过滤盖、加热器、配件泵、放大器、电泳箱、内插管、注入阀、纯化柱、过滤器、48i配件、色谱柱、fid模块、钳口盖、盖组件