Overview
Rectal fibroblasts are mesenchymal cells native to the rectal submucosa and connective tissue layers. These cells are pivotal in maintaining tissue integrity through continuous extracellular matrix (ECM) remodeling. Unlike epithelial cells, fibroblasts exhibit spindle-shaped morphology and express specific markers like vimentin and fibroblast activation protein (FAP). In biomedical contexts, rectal fibroblasts are increasingly studied for their role in inflammatory bowel diseases (IBD), colorectal cancer stroma, and pelvic floor disorders. Their ability to modulate immune responses and influence epithelial cell behavior makes them valuable for both basic research and therapeutic development.
Key Features
Functionally, rectal fibroblasts produce ECM components such as collagens I/III, fibronectin, and elastin, which provide structural support to rectal tissue. They also secrete matrix metalloproteinases (MMPs) for tissue remodeling and cytokines like TGF-β to regulate inflammation. A distinguishing feature is their phenotypic plasticity—under pathological conditions (e.g., fibrosis or tumors), they can transform into myofibroblasts with contractile properties. Research-grade rectal fibroblasts typically exhibit >90% viability and are tested for microbial contamination to ensure experimental reliability.
Application Areas
In drug discovery, rectal fibroblasts serve as in vitro models for testing anti-fibrotic therapies and IBD treatments. Their response to inflammatory stimuli helps evaluate drug efficacy in conditions like ulcerative colitis. Tissue engineering applications include biofabrication of rectal grafts for surgical reconstruction. Combined with biomaterial scaffolds, these cells can regenerate functional connective tissue. Additionally, cancer researchers study fibroblast-tumor cell interactions to develop stromal-targeted therapies for colorectal carcinoma.
Precautions
Handling requires biosafety level 2 (BSL-2) protocols due to potential pathogen exposure from human-derived cells. Cryopreserved vials must be thawed rapidly in 37°C water baths to prevent ice crystal damage. Culture conditions demand specific media (e.g., DMEM/F12 with 10% FBS) and low-oxygen environments mimicking physiological conditions. Ethical procurement mandates documented donor consent and compliance with institutional review board (IRB) guidelines, particularly for cells used in clinical applications.
B2B Procurement Guide
Reputable suppliers provide certificates of analysis (CoA) detailing cell purity, doubling time, and absence of HIV/HBV/HCV. Bulk purchases (10+ vials) often attract 15–20% discounts, while custom services (e.g., gene-edited lines) incur higher costs. Lead times vary: off-the-shelf primary cells ship within 1–2 weeks, whereas cultured batches may require 4–6 weeks. For regulatory-sensitive projects, opt for ISO 13485-certified vendors offering full traceability from donor to delivery.
Related Manufacturers
- 主营：原代细胞、ELISA试剂盒、ELISA检测试剂盒、大鼠直肠平滑肌细胞、重组蛋白、天然蛋白、活性蛋白、基础培养基、完全培养基、抗体、标记抗体、人检测试剂盒、大鼠检测试剂盒、标记细胞、永生化细胞
- 主营：科研试剂盒、酶免试剂盒、elisa试剂盒、细胞、生物试剂、进口ELISI试剂盒、PCR试剂盒
- 主营：ELISA试剂盒、PCR试剂盒、科研试剂
- 主营：igg抗体、糖蛋白、试剂盒、小鼠胚胎成纤维细胞、脂蛋白、人瘦素、粗纤维、猪睾酮、烯二酮、nad激酶、测试盒、牛瘦素、牛胆酸、原果胶、磷酸酶、犬肾素、含水量、人蛋白、甜菜碱、igm抗体、dna抗体、磷脂酶、大鼠超、犬叶酸、人胆酸、样受体
- 主营：elisa 试剂盒、Elisa试剂盒、进口试剂盒、国产试剂盒、试剂、细胞、ELISA检测试剂盒、酶联免疫法、酶联免疫试剂盒、酶活性检测、酶标仪检测
- 主营：试剂盒、人25羟基维生素、人低氧诱导因子
- 主营：科研试剂盒、酶免试剂盒、elisa试剂盒、生化试剂盒、细胞、生物试剂、进口ELISA试剂盒、PCR试剂盒、生化测试盒
- 主营：检测方、钠尿肽、试剂盒、同源物、ep300检测、结合蛋白、大鼠c-肽(、微球蛋白、抗胰蛋白酶、淋巴瘤因子、蛋白磷酸酶、死亡激动剂、核心域蛋白1、大鼠e选择素、大鼠janus激酶、激活蛋白激酶、大鼠ⅱ型胶原、大鼠ⅳ型胶原、大鼠klotho蛋白、细胞激活因子、大鼠ⅲ型胶原、大鼠nadh脱氢酶1、大鼠ⅵ型胶原α1、elisa 检测试剂盒
- 主营：科研试剂盒、酶免试剂盒、elisa试剂盒、细胞、生物试剂、进口ELISA试剂盒、PCR试剂盒、生化测试盒
- 主营：ELISA试剂盒、PCR试剂盒、生化试剂盒、小鼠胚胎成纤维细胞、科研试剂
- 主营：ELISA试剂盒、抗体
- 主营：微生物、昆虫细胞、高纯度原料、肿瘤成纤维细胞、完全培养基
- 主营：ELISA、分光光度法、微量法、ELISA试剂盒、大鼠试剂盒、小鼠试剂盒、人试剂盒、植物试剂盒、酶联免疫法、MEGAZYME、大鼠ELISA试剂盒、小鼠ELISA试剂盒、人ELISA试剂盒、植物ELISA 试剂盒、白介素试剂盒、细胞因子试剂盒、激素试剂盒、科研试剂盒、化学试剂、检测试剂盒、土壤试剂盒、土壤酶活性检测
- 主营：ELISA试剂盒、小鼠ELISA试剂盒、大鼠ELISA试剂盒、生化检测试剂盒、酶联免疫检测试剂盒、人ELISA检测试剂盒、植物检测试剂盒、兔ELISA试剂盒、猪elisa检测试剂盒、犬ELISA试剂盒、科研ELISA检测试剂盒
- 主营：膀胱癌细胞、人胚肾细胞、前列腺癌细胞
- 主营：基质胶、试剂盒、细胞株、脑类器官、重组蛋白、成体干细胞、细胞培养基