Overview
Recombinant Human Stem Cell Factor (rhSCF) is a biologically active cytokine produced through recombinant DNA technology. It mimics the natural stem cell factor (SCF), also known as Kit ligand, which plays a critical role in hematopoiesis by binding to the c-Kit receptor on hematopoietic stem cells. rhSCF is widely used in research laboratories and clinical settings to support the growth and differentiation of stem cells, particularly in bone marrow transplantation and ex vivo cell expansion protocols. Its recombinant form ensures high purity and consistency, making it suitable for standardized applications.
Physical and Chemical Properties
rhSCF is typically supplied as a lyophilized powder or sterile solution with a molecular weight of approximately 18-20 kDa when glycosylated. The protein is stable under recommended storage conditions but may degrade if exposed to repeated freeze-thaw cycles or improper handling. Solubility is high in aqueous buffers, and the protein retains its biological activity when reconstituted correctly. Analytical techniques such as SDS-PAGE and HPLC are commonly used to verify purity, which should exceed 95% for research-grade products. Endotoxin levels must be minimized (<1 EU/µg) to avoid unwanted immune responses in cell cultures or clinical applications.
Main Applications
rhSCF is primarily utilized in stem cell research to promote the proliferation and survival of hematopoietic progenitor cells. It is often combined with other cytokines like granulocyte colony-stimulating factor (G-CSF) to enhance cord blood or bone marrow stem cell expansion. Clinically, rhSCF has been investigated for treating bone marrow failure syndromes and improving engraftment in transplant patients. It also finds use in regenerative medicine studies, where it supports the growth of tissue-specific stem cells. In biotechnology, rhSCF is employed to maintain stem cell cultures for drug screening and disease modeling.
Safety and Storage
Proper storage of rhSCF is critical to maintain its bioactivity. Lyophilized powder should be kept at -20°C, while reconstituted solutions must be aliquoted and stored at -80°C to prevent degradation. Avoid repeated freeze-thaw cycles, which can denature the protein. Safety precautions include using sterile techniques during handling to prevent contamination. Although rhSCF is generally safe for in vitro use, it may cause allergic reactions in sensitive individuals. Always wear appropriate personal protective equipment (PPE) and follow institutional biosafety guidelines when working with recombinant proteins.
B2B Procurement Guide
When procuring rhSCF, prioritize suppliers with proven track records in recombinant protein production. Key specifications to verify include purity (>95% by SDS-PAGE), endotoxin levels (<1 EU/µg), and biological activity (e.g., ED50 in cell proliferation assays). For clinical or GMP applications, ensure the product complies with regulatory standards (e.g., FDA, EMA). Bulk purchasing may offer cost savings, but confirm stability data for long-term storage. Request certificates of analysis (CoA) and consider third-party testing for critical batches. Lead times can vary; plan orders in advance to avoid project delays.
Related Manufacturers
- 主营：铜肽、玻色因、蛇毒肽、干细胞因子、九肽、生长因子、寡肽、芋螺肽、六胜肽、肌肽、表皮生长因子、类蛇毒三肽、蓝铜肽、生物素三肽、三肽-1铜、二肽、乙酰基四肽-5、六肽-10、乙酰基四肽、肉豆蔻酰五肽-17、肉豆蔻酰六肽-16、EGF、乙酰基六肽、棕榈酰寡肽、三肽、五肽
- 主营：人干细胞因子、ELISA试剂盒、PCR试剂盒、科研试剂
- 主营：ELISA试剂盒、PCR试剂盒、生化试剂盒、干细胞因子受体ELISAKit、蛋白、抗体、细胞、检测试剂盒
- 主营：科研试剂盒、酶免试剂盒、elisa试剂盒、马干细胞因子、生化试剂盒、细胞、生物试剂、进口ELISA试剂盒、PCR试剂盒、生化测试盒
- 主营：igg抗体、糖蛋白、试剂盒、人干细胞因子、脂蛋白、人瘦素、粗纤维、猪睾酮、烯二酮、nad激酶、测试盒、牛瘦素、牛胆酸、原果胶、磷酸酶、犬肾素、含水量、人蛋白、甜菜碱、igm抗体、dna抗体、磷脂酶、大鼠超、犬叶酸、人胆酸、样受体
- 主营：检测方、钠尿肽、试剂盒、马干细胞因子、同源物、ep300检测、结合蛋白、大鼠c-肽(、微球蛋白、抗胰蛋白酶、淋巴瘤因子、蛋白磷酸酶、死亡激动剂、核心域蛋白1、大鼠e选择素、大鼠janus激酶、激活蛋白激酶、大鼠ⅱ型胶原、大鼠ⅳ型胶原、大鼠klotho蛋白、细胞激活因子、大鼠ⅲ型胶原、大鼠nadh脱氢酶1、大鼠ⅵ型胶原α1、elisa 检测试剂盒
- 主营：试剂盒、elisa试剂盒、细胞、pcr试剂盒
- 主营：ELISA试剂盒、PCR试剂盒、生化试剂盒、蛋白、抗体、细胞
- 主营：ELISA试剂盒、PCR试剂盒、生化试剂盒、人干细胞因子、科研试剂
- 主营：人干细胞因子、ELISA试剂盒、抗体
- 主营：科研试剂盒、酶免试剂盒、elisa试剂盒、人干细胞因子受体、细胞、生物试剂、进口ELISA试剂盒、PCR试剂盒、生化测试盒
- 主营：ELISA试剂盒、小鼠ELISA试剂盒、大鼠ELISA试剂盒、生化检测试剂盒、酶联免疫检测试剂盒、人ELISA检测试剂盒、植物检测试剂盒、兔ELISA试剂盒、猪elisa检测试剂盒、犬ELISA试剂盒、科研ELISA检测试剂盒
- 主营：elisa 试剂盒、Elisa试剂盒、进口试剂盒、干细胞因子ELISA试剂盒、国产试剂盒、试剂、细胞、ELISA检测试剂盒、酶联免疫法、酶联免疫试剂盒、酶活性检测、酶标仪检测
- 主营：膀胱癌细胞、人胚肾细胞、前列腺癌细胞
- 主营：人干细胞因子、检测试剂盒