Overview
Rat histones are highly conserved alkaline proteins that package DNA into nucleosomes, the fundamental units of chromatin. These proteins play critical roles in gene regulation through post-translational modifications (PTMs) such as acetylation and methylation. Five main histone classes exist in rats (H1, H2A, H2B, H3, H4), with core histones forming an octamer around which 147 base pairs of DNA wrap. As model organisms share conserved histone structures, rat histones are widely used in comparative epigenetics studies.
Physical and Chemical Properties
Rat histones exhibit high isoelectric points (pI 10-12) due to abundant lysine and arginine residues, enabling strong DNA binding. They maintain stability in physiological pH ranges but degrade under extreme conditions. Post-purification, histones are typically lyophilized and require reconstitution in specialized buffers (e.g., containing DTT to prevent oxidation). Dynamic PTMs significantly alter their charge and interaction properties without changing primary sequence.
Main Applications
In biomedical research, rat histones serve as critical tools for studying chromatin remodeling, epigenetic inheritance, and transcriptional regulation. Modified histones help investigate disease mechanisms like cancer progression. Pharmaceutical companies utilize histone arrays to screen compounds targeting epigenetic enzymes (HDACs, HATs). Recent advances in ChIP-seq techniques have increased demand for species-specific histones in proteomics workflows.
Safety and Storage
While non-toxic, histone preparations may contain trace contaminants from purification processes. Standard laboratory precautions (gloves, goggles) are recommended during handling. Lyophilized histones remain stable for years at -80°C when desiccated. Reconstituted aliquots should be used within weeks and undergo limited freeze-thaw cycles to prevent aggregation. Buffers should include protease inhibitors for long-term solutions.
B2B Procurement Guide
Researchers should verify vendor documentation for: 1) Source validation (rat liver/thymus), 2) PTM characterization mass spec reports, 3) Endotoxin levels (<1 EU/μg for cell studies). Bulk buyers can request custom modifications (acetylated K9, trimethylated K27, etc.). Leading suppliers provide COAs with HPLC purity (>95% recommended) and provide batch-specific modification profiles. Consider requesting stability data for long-term projects.
Related Manufacturers
- 主营：增氧剂、消毒液、除雪剂、处理剂、消毒剂、硫酸铵、aes洗涤、润湿剂、催化剂、漂白粉、冲施肥、亚硝酸、尿素粉、全水溶、硫酸钠、氧化剂、防霉剂、清洗剂、漂白剂、片水产、洗涤剂、尿素桶、熟石灰、碳酰胺、碳酸钠
- 主营：卡拉胶、乳胶漆、聚乙烯、消泡剂、固化剂、分散剂、苯丙乳液、多环颜料、酸催化剂、颜料助剂、水性涂料、涂料助剂、乳品饮料、聚醛树脂、酞青颜料、水性木漆、烤漆助剂、果冻酱料、无机颜料、防腐涂料、高性能颜料、有机膨润土、糖果巧克力、涂料添加剂、水性润湿剂
- 主营：牛骨胶、胶黏剂、工业明胶、电镀光亮剂、提炼胶颗粒、增稠高粘度骨胶
- 主营：工业骨胶、工业明胶、牛胶、骨胶粉、明胶、工业蛋白、蛋白粉、皮胶、骨胶、牛胶粉、工业胶、水胶、牛骨胶、小颗粒胶、动物胶
- 主营：大鼠组蛋白H2bELISAKit、ELISA试剂盒、PCR试剂盒、科研试剂
- 主营：科研试剂盒、酶免试剂盒、elisa试剂盒、大鼠组蛋白甲基转移酶、生化试剂盒、细胞、生物试剂、进口ELISA试剂盒、PCR试剂盒、生化测试盒
- 主营：ELISA试剂盒、抗体
- 主营：ELISA试剂盒、PCR试剂盒、生化试剂盒、大鼠组蛋白H2bELISAKit、科研试剂
- 主营：大鼠组蛋白去乙酰化酶、检测试剂盒
- 主营：scl-70igg、人1α羟、试剂盒、大鼠11β、大鼠8羟基、大鼠25羟基、葡聚糖、16α羟基、1iga抗体、胶原c端、2-he2elisa、1igm抗体、25二羟基、17羟孕酮、dna糖苷酶、5α还原酶、研启生物、去氢血栓、人胰岛素、胶原n末端、25羟基维生、2，5寡腺苷、11脱氢血栓、羟基壬烯醛、三磷酸肌醇
- 主营：ELISA检测试剂盒
- 主营：大鼠组蛋白去乙酰化酶、试剂盒
- 主营：Elisa试剂盒、生化试剂盒、蛋白试剂盒、细胞试剂盒
- 主营：大鼠组蛋白H2A、试剂盒、信号调节激酶、人转谷氨酰胺酶
- 主营：ELISA试剂盒、生化检测试剂盒、PCR试剂盒、大鼠ELISA试剂盒、小鼠ELISA试剂盒、兔ELISA试剂盒、鸡ELISA试剂盒
- 主营：大鼠组蛋白H2bELISA、ELISA试剂盒、PCR试剂盒、科研试剂
- 主营：elisa试剂盒、人elisa试剂盒、小鼠elisa试剂盒、大鼠elisa试剂盒、植物elisa试剂盒、鱼elisa试剂盒、猪elisa试剂盒、牛elisa试剂盒、鸡elisa试剂盒、兔elisa试剂盒、犬elisa试剂盒
- 主营：试剂盒、ELISA试剂盒、PCR试剂盒、科研细胞系
- 主营：ELISA试剂盒、PCR试剂盒、生化试剂盒、大鼠组蛋白H2bELISAKit、科研试剂