Overview
Rat Growth Factor (RGF) refers to a group of proteins, such as EGF, FGF, or IGF, that regulate cellular growth and repair in rats. These factors are critical tools in preclinical studies, particularly in rodent-based models of wound healing, oncology, and developmental biology. Produced via recombinant DNA technology or extracted from rat tissues, RGFs are standardized by bioactivity units. Their purity and specificity make them indispensable for researchers requiring species-matched reagents to avoid cross-species reactivity issues.
Physical and Chemical Properties
RGFs are typically stable as lyophilized powders but degrade rapidly in solution if improperly stored. They exhibit optimal activity at physiological pH (7.2-7.4) and lose function above 60°C due to protein denaturation. Molecular weights vary by subtype: epidermal growth factor (EGF) is ~6 kDa, while fibroblast growth factors (FGFs) range up to 30 kDa. Most RGFs require carrier proteins (e.g., BSA) in low-concentration solutions to prevent adsorption to surfaces.
Main Applications
In biomedical research, RGFs are used to enhance cell proliferation in primary rat cell cultures, including hepatocytes and neural stem cells. They are also applied in 3D bioprinting studies to simulate tissue microenvironments. Pharmaceutical companies employ RGFs in drug efficacy testing, particularly for compounds targeting growth pathways. Their use in veterinary research focuses on improving livestock cloning techniques through optimized cell culture conditions.
Safety and Storage
RGFs require handling under Biosafety Level 1 or 2 conditions, depending on the source material. Lyophilized forms should be reconstituted with sterile, endotoxin-free buffers and aliquoted to minimize contamination risks. Long-term storage at -80°C preserves activity for years, while working solutions can be kept at 4°C for up to 2 weeks with protease inhibitors. Always centrifuge lyophilized vials before opening to prevent powder dispersion.
B2B Procurement Guide
When sourcing RGFs, prioritize suppliers providing detailed Certificates of Analysis (CoA) with batch-specific activity data. Key metrics include endotoxin levels (<1 EU/µg), sterility testing results, and species validation (rat-specific isoforms). Bulk purchasers should negotiate stability data for large-volume orders and consider custom formulations (e.g., carrier-free options for in vivo studies). Leading manufacturers include PeproTech, R&D Systems, and Sino Biological, with lead times of 2-4 weeks for specialty isoforms.
Related Manufacturers
- 主营：科研试剂盒、酶免试剂盒、elisa试剂盒、细胞、生物试剂、进口ELISI试剂盒、PCR试剂盒
- 主营：ELISA试剂盒、PCR试剂盒、科研试剂
- 主营：elisa试剂盒、检测试剂盒、PCR试剂盒
- 主营：ELISA试剂盒、PCR试剂盒、生化试剂盒、脂蛋白、关基因、亲环素、钾通道、列抗体、ntp溶液、蛋白酶、脑蛋白、病毒抗、蛋白质、239抗体、dtt溶液、科研试剂
- 主营：大鼠生长因子抗体、elisa试剂盒
- 主营：gal7elisa、bmp7elisa、ctsbelisa、bmp2elisa、ang2elisa、fgf7elisa、prb4elisa、cd80elisa、bmp4elisa、bmp5elisa、gal2elisa、试剂盒、ccl7elisa、ctsdelisa、dkk3elisa、heat检测、bssp检测、fstl1elisa、edm1检测、ccn1检测、cd54检测、mic1检测、cd30lelisa、ccl16elisa、il10检测
- 主营：蛋白酶、淀粉酶、果胶酶、胰酵素、崩溃酶、蜗牛酶、乳糖酶、肌酸激酶、葡球菌酶、绿色木霉、心肌黄酶、亮抑酶肽、纤维素酶、木聚糖酶、-glucosidase、亮抑肽酶、硫代辅酶、枯草杆菌、羧化辅酶、氯化酵素、脆壁质酶、细胞溶解酶、乙醇脱氢酶、透明质酸酶、氯化溶菌酶
- 主营：l谷氨酸、d谷氨酸、l亮氨酸、人补体7、人补体1q、largininehcl、人钙调素、人雌三醇、人唾液酸、人多巴胺、人强啡肽、bocl丙氨酸、l异亮氨酸、猪d二聚体、d天冬氨酸、l天冬氨酸、人白三烯c4、猪白介素12、pcdh1试剂盒、elisa试剂盒、dl半胱氨酸、人磷脂酶a2、dl谷氨酰胺、人降钙素原、人轮状病毒
- 主营：ELISA试剂盒、ELISA检测试剂盒、小鼠ELISA试剂盒、大鼠ELISA试剂盒、人ELISA试剂盒、试剂盒elisa
- 主营：人流感、m117-500g、脂蛋白、大鼠生长因子、0189-100g、猪睾酮、人胰岛、赖氨酸、柚皮素、人新生、人蛋白、e122-100g、柚皮苷、人戊肝、磷脂酶、蛋白酶、fishelisa、皂苷rg3、酮戊二、人轮状、人胆酸、j636-100g、胰多肽、厚朴酚、噻唑蓝、甜菊苷
- 主营：ELISA试剂盒、小鼠ELISA试剂盒、细胞因子检测试剂盒、大鼠生长因子、人ELISA检测试剂盒、姐检测试剂盒、酶免检测试剂盒、生化试剂盒、酶联免疫吸附测定试剂盒
- 主营：igg抗体、糖蛋白、试剂盒、大鼠超、脂蛋白、人瘦素、粗纤维、猪睾酮、烯二酮、nad激酶、测试盒、牛瘦素、牛胆酸、原果胶、磷酸酶、犬肾素、含水量、人蛋白、甜菜碱、igm抗体、dna抗体、磷脂酶、犬叶酸、人胆酸、样受体
- 主营：elisa 试剂盒、Elisa试剂盒、进口试剂盒、国产试剂盒、试剂、细胞、ELISA检测试剂盒、酶联免疫法、酶联免疫试剂盒、酶活性检测、酶标仪检测
- 主营：大鼠elisa试剂盒、试剂盒、人25羟基维生素、人低氧诱导因子
- 主营：科研试剂盒、酶免试剂盒、elisa试剂盒、生化试剂盒、细胞、生物试剂、进口ELISA试剂盒、PCR试剂盒、生化测试盒