Overview
Rat Calbindin, also known as Calbindin-D28k, is a calcium-binding protein predominantly expressed in the central nervous system and kidneys of rats. It belongs to the EF-hand protein family and plays a vital role in buffering intracellular calcium concentrations, thereby protecting neurons from calcium-induced cytotoxicity. This protein is widely used as a biomarker in neuroscience research, particularly for identifying specific neuronal populations. Its high affinity for calcium ions and stability under physiological conditions make it an excellent tool for studying calcium-dependent cellular processes.
Physical and Chemical Properties
Rat Calbindin has a molecular weight of approximately 28 kDa and typically appears as a white to off-white lyophilized powder. It is soluble in aqueous buffers and maintains its structural integrity across a wide pH range, making it suitable for various experimental conditions. The protein contains multiple EF-hand motifs that enable its calcium-binding capacity. These motifs undergo conformational changes upon calcium binding, which is crucial for its function in calcium signaling and homeostasis. The protein's stability and solubility are key factors in its widespread use in laboratory settings.
Main Applications
Rat Calbindin is primarily used in neuroscience research to study calcium signaling pathways and neuronal protection mechanisms. It serves as a selective marker for certain neuronal subtypes, such as Purkinje cells in the cerebellum and specific interneurons in the hippocampus. In addition to its research applications, Rat Calbindin is employed in immunohistochemistry to visualize calcium-binding neurons in tissue sections. Its role in calcium homeostasis also makes it a subject of interest in studies related to neurodegenerative diseases and renal physiology.
Safety and Storage
When handling Rat Calbindin, standard laboratory safety protocols should be followed. Use personal protective equipment, including gloves and lab coats, to avoid direct contact. In case of accidental exposure, rinse thoroughly with water and seek medical advice if necessary. For long-term storage, the protein should be kept at -20°C in a dry environment. Avoid repeated freeze-thaw cycles to prevent degradation. Aliquotting the protein upon receipt can help maintain its stability and extend its shelf life.
B2B Procurement Guide
When procuring Rat Calbindin for B2B purposes, it is essential to verify the purity and specificity of the product. Look for suppliers that provide detailed certificates of analysis, including information on purity (typically ≥90%), endotoxin levels, and batch-specific data. Consider the intended application when selecting the product form (e.g., lyophilized powder or solution). Ensure that the supplier can guarantee proper storage and shipping conditions to maintain the protein's integrity. Comparing prices from multiple vendors can help secure the best deal without compromising quality.
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