Overview
Rat Bladder Matrix is a biologically derived scaffold produced through decellularization processes that remove cellular components while preserving the native extracellular matrix (ECM) architecture. This biomaterial maintains critical structural proteins like collagen IV and laminin that support cell adhesion and differentiation. In research settings, RBM serves as a gold standard for studying bladder regeneration due to its species-specific relevance. The matrix's microarchitecture closely resembles human bladder ECM, making it valuable for translational studies in urology and regenerative medicine.
Physical and Chemical Properties
RBM exhibits a characteristic pore size range of 50-200 μm, with interconnected porosity exceeding 90% to facilitate cell infiltration. The matrix demonstrates tensile strength of 0.5-1.5 MPa and elongation at break of 50-80%, properties suitable for surgical handling and physiological bladder expansion. Biochemically, processed RBM typically contains <5% residual DNA while preserving >90% of native glycosaminoglycans (GAGs). The material shows stable thermogravimetric properties up to 60°C, with complete decomposition occurring around 300°C. FTIR analysis confirms retention of amide I/II bands indicative of intact collagen structures.
Main Applications
Primary use cases include bladder augmentation models in rodents, where RBM scaffolds demonstrate superior urothelial cell repopulation compared to synthetic materials. Researchers also utilize RBM for studying smooth muscle regeneration and neural reinnervation patterns post-implantation. Beyond urology, RBM finds application in general wound healing research due to its angiogenic properties. Some laboratories employ it as a growth substrate for stem cell differentiation studies, particularly for mesenchymal stem cells toward urothelial lineages.
Safety and Storage
Properly decellularized RBM shows minimal immunogenicity in rat models, but batch-to-batch variability requires validation through ELISA testing for residual α-Gal epitopes. Gamma-irradiated (25-35 kGy) products offer optimal sterility assurance for implantation studies. For storage, lyophilized matrices maintain stability for 2+ years at -20°C in vacuum-sealed packaging. Rehydrated scaffolds should be used within 24 hours when stored at 4°C in antibiotic-containing saline. Repeated freeze-thaw cycles degrade mechanical properties and should be avoided.
B2B Procurement Guide
Reputable suppliers should provide certificates of analysis including: (1) residual DNA quantification, (2) endotoxin levels (<0.5 EU/mg), (3) sterility testing results, and (4) mechanical property specifications. For GMP-grade materials, request documentation of source animal health status and tissue procurement protocols. Lead times for custom-sized RBM scaffolds typically range 4-8 weeks. Bulk purchases (10+ units) often qualify for 15-20% discounts. Consider requesting sample batches for cell compatibility testing before large orders. Some suppliers offer pre-seeded options with relevant cell types at premium pricing.
Related Manufacturers
- 主营：试剂盒、ELISA试剂盒、PCR试剂盒、科研细胞系