Overview
Protein C is a critical component of the blood coagulation system, primarily synthesized in the liver as an inactive precursor. Upon activation by thrombin and thrombomodulin, it becomes Activated Protein C (APC), which exhibits potent anticoagulant and anti-inflammatory properties. APC degrades factors Va and VIIIa, inhibiting excessive clot formation. Its role extends beyond coagulation, influencing endothelial barrier function and cellular apoptosis. Deficiencies in Protein C, whether hereditary or acquired, are associated with heightened thrombotic risks. Congenital Protein C deficiency is a rare autosomal dominant disorder, often presenting with venous thromboembolism in young adults. Acquired deficiencies may result from liver disease, vitamin K deficiency, or disseminated intravascular coagulation (DIC).
Physical and Chemical Properties
Protein C is a glycoprotein with a molecular weight of approximately 62,000 Da. It consists of a light chain and a heavy chain linked by disulfide bonds. The protein undergoes post-translational modifications, including gamma-carboxylation of glutamic acid residues, which are essential for its calcium-dependent binding to phospholipid membranes. In its purified form, Protein C is typically a lyophilized powder or liquid solution. It is soluble in water and stable under recommended storage conditions. The activity of Protein C is highly dependent on its structural integrity, making proper handling and storage critical to maintain its biological function.
Main Applications
Protein C is primarily used in clinical settings to treat hereditary or acquired deficiencies, reducing the risk of thrombotic events. Purified Protein C concentrates are administered to patients with severe deficiencies, particularly neonates with life-threatening purpura fulminans. Activated Protein C (APC) has been investigated for its therapeutic potential in sepsis, though its use remains controversial due to mixed clinical trial outcomes. Research applications include studying coagulation pathways, endothelial function, and inflammation. Protein C assays are also employed in diagnostic laboratories to evaluate thrombotic risk and monitor anticoagulant therapy. Its cytoprotective effects are a focus of ongoing research in ischemic and inflammatory conditions.
Safety and Storage
Protein C requires careful handling to preserve its activity. Lyophilized forms should be reconstituted with sterile water or buffer, avoiding vigorous shaking to prevent denaturation. Solutions should be aliquoted to minimize freeze-thaw cycles and stored at -20°C or below for long-term stability. Safety precautions include wearing appropriate personal protective equipment (PPE) when handling lyophilized powders or concentrated solutions. Contamination must be avoided, as it can compromise the protein's efficacy. For clinical use, ensure products are sourced from reputable suppliers and comply with regulatory standards, such as those set by the FDA or EMA.
B2B Procurement Guide
When procuring Protein C for B2B purposes, prioritize suppliers with documented quality control measures. Key considerations include purity (typically >95% by SDS-PAGE), specific activity (measured in IU/mg), and endotoxin levels (<1 EU/mg for clinical-grade products). Certificates of Analysis (CoA) should accompany each batch, detailing analytical methods and results. Bulk purchases may offer cost advantages, but verify storage capacity and shelf-life requirements. For research applications, recombinant Protein C may be a viable alternative to plasma-derived forms. Engage with suppliers to discuss customization options, such as conjugation or labeling, for specialized applications.
Related Manufacturers
- 主营：科研试剂盒、酶免试剂盒、elisa试剂盒、细胞、生物试剂、进口ELISI试剂盒、PCR试剂盒
- 主营：花生酸、碳酸钠、单氟磷酸钠、火麻仁蛋白、丁基羟基茴香醚、鱼大豆酵母原料
- 主营：ELISA试剂盒、PCR试剂盒、生化试剂盒、蛋白、抗体、细胞、检测试剂盒
- 主营：蛋白激酶C、ELISA试剂盒、PCR试剂盒、科研试剂
- 主营：pcr试剂盒、试剂盒、elisa试剂盒、细胞
- 主营：ELISA试剂盒、PCR试剂盒、生化试剂盒、蛋白、抗体、细胞
- 主营：ELISA试剂盒、抗体
- 主营：α2巨球、素d合成、肌醇抗体、酶cγ链、纤连蛋白、抗组蛋白、蛋白样环、敏感蛋白、蛋白重链、乳球蛋白、蛋白激酶、钙调蛋白、pcr检测试剂、牛骨钙素、金属肽酶、钩端螺旋、高血糖素、小鼠α1抗、ELISA试剂盒、生化检测试剂
- 主营：大鼠粘胶蛋白、试剂盒、ELISA试剂盒、ELISA检测试剂盒
- 主营：试剂盒、elisa试剂盒、ELiSA试剂盒、科研试剂盒、试剂
- 主营：ELISA试剂盒、小鼠ELISA试剂盒、大鼠ELISA试剂盒、生化检测试剂盒、酶联免疫检测试剂盒、人ELISA检测试剂盒、植物检测试剂盒、兔ELISA试剂盒、猪elisa检测试剂盒、犬ELISA试剂盒、科研ELISA检测试剂盒
- 主营：Elisa试剂盒、检测试剂盒、人elisa试剂盒、慢肌肌钙蛋白C、鼠elisa试剂盒、PCR检测试剂盒、生物试剂盒
- 主营：大鼠粘胶蛋白、检测试剂盒
- 主营：抑制物、试剂盒、释放因子、糖蛋白、炎性蛋白、钙黏蛋白、成孔蛋白、免疫球蛋白、二聚化蛋白、人潜伏膜蛋白、人色氨酰、刺激因子、生长因子、武装因子、淋巴细胞、趋化因子、人促睡眠肽、节苷脂抗体、人精氨酸酶、人β葡糖苷酶、人β内酰胺酶、肝炎病毒抗体、活化黏附因子、凋亡抑制因子、人二氢嘧啶酶
- 主营：白细胞介素、生物试剂、甲状腺素、心肌肌钙蛋白、活性氧簇、促甲状腺激素、孕酮、促黄体生成素、检测试剂盒、植物试剂盒、试剂盒、实验室试剂、Elisa、定量分析、定性分析、酶联免疫试剂盒、科研试剂盒、免费代测