PR3 ELISA Kit

Updated: 2026-09-11

Overview

The PR3 ELISA Kit is an enzyme-linked immunosorbent assay designed to detect Proteinase 3 (PR3) antibodies in human serum. PR3 is a serine protease associated with autoimmune diseases, particularly granulomatosis with polyangiitis (GPA). The kit is widely used in clinical diagnostics and research laboratories due to its high accuracy and reproducibility. The kit typically includes pre-coated microplates, detection antibodies, substrates, and buffers. It operates on the principle of antigen-antibody interaction, where PR3 antibodies in the sample bind to immobilized PR3 antigens. A colorimetric reaction then quantifies the antibody levels, providing measurable results.

Physical and Chemical Properties

The PR3 ELISA Kit consists of liquid reagents stored in buffered solutions to maintain stability. The appearance is usually clear or slightly colored, depending on the components. The kit is water-soluble and designed for use in aqueous environments, such as serum or plasma samples. Storage conditions are critical for maintaining reagent integrity. The kit must be kept at 2-8°C and protected from freezing or exposure to direct sunlight. Proper storage ensures consistent performance and extends the shelf life, typically up to 12 months from the date of manufacture.

Main Applications

The primary application of the PR3 ELISA Kit is the diagnosis and monitoring of autoimmune diseases, especially granulomatosis with polyangiitis (GPA). Elevated levels of PR3 antibodies are a hallmark of this condition, making the kit a valuable tool for clinicians. Beyond clinical diagnostics, the kit is also used in research settings to study PR3-related autoimmune mechanisms. It aids in understanding disease progression and evaluating the efficacy of therapeutic interventions. Its high specificity minimizes cross-reactivity with other antibodies, ensuring reliable results.

Safety and Storage

Handling the PR3 ELISA Kit requires standard laboratory safety precautions. Wear gloves and protective eyewear to avoid contact with reagents, which may contain preservatives or other chemicals. In case of skin or eye exposure, rinse immediately with water. Proper storage is essential for maintaining kit performance. Store all components at 2-8°C and avoid repeated freeze-thaw cycles. Ensure the kit is used before the expiration date, and discard any compromised reagents to prevent inaccurate results.

B2B Procurement Guide

When procuring PR3 ELISA Kits, consider factors such as kit sensitivity, specificity, and compatibility with your laboratory equipment. Verify the manufacturer's reputation and request validation data to ensure reliability. Pricing varies based on the number of tests per kit and the supplier. Bulk purchases may offer cost savings, but always check expiration dates to avoid waste. Additionally, inquire about technical support and troubleshooting services provided by the supplier.