Overview
Plant DNA Extraction Buffer is a critical reagent in molecular biology designed to isolate DNA from plant tissues efficiently. It combines chemical agents to break down rigid plant cell walls, neutralize nucleases, and protect DNA integrity during extraction. The buffer is widely used in laboratories for applications ranging from academic research to agricultural biotechnology. Its formulation varies by manufacturer but generally includes detergents (e.g., CTAB or SDS), salts (e.g., NaCl), and chelators (e.g., EDTA) to optimize DNA yield and purity.
Physical and Chemical Properties
The buffer appears as a clear or slightly cloudy liquid with a neutral to slightly alkaline pH. Its density typically ranges from 1.0 to 1.2 g/cm³, depending on solute concentration. The solution is water-soluble and often contains stabilizing agents like Tris-HCl. Key functional components include surfactants to disrupt lipid membranes, chaotropic salts to denature proteins, and antioxidants to prevent DNA oxidation. These properties ensure effective lysis of plant cells while minimizing DNA shearing and enzymatic degradation.
Main Applications
Primary uses include genomic DNA extraction for PCR amplification, restriction digestion, and next-generation sequencing. It is indispensable in plant genetic engineering, species identification, and phylogenetic studies. In agriculture, the buffer aids in marker-assisted breeding and GMO detection. Forensic labs employ it for botanical evidence analysis. Custom formulations are available for challenging samples like polysaccharide-rich or phenolic-laden tissues.
Safety and Storage
The buffer may contain irritants such as detergents or β-mercaptoethanol. Always use PPE (gloves, goggles) and work in a fume hood if volatile components are present. Avoid skin contact and inhalation. Store at 2-8°C in opaque bottles to prevent photodegradation of sensitive reagents. Shelf life is typically 6-12 months when stored properly. Discard if precipitation or discoloration occurs.
B2B Procurement Guide
When sourcing, confirm the buffer’s compatibility with your extraction method (e.g., silica-column vs. alcohol precipitation). Bulk purchases (5+ liters) often reduce costs by 15-30%. Request certificates of analysis for lot-to-lot consistency. Evaluate suppliers based on technical support, buffer customization options, and ISO certification. Leading manufacturers include Thermo Fisher, Qiagen, and Sigma-Aldrich. For specialized applications (e.g., ancient DNA), seek buffers with enhanced nuclease inhibition.
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