Overview
Phosphorylated Src (p-Src) is a post-translationally modified variant of the Src kinase, a non-receptor tyrosine kinase critical in cellular signaling. Src phosphorylation, typically at Tyr416, enhances its enzymatic activity and role in pathways regulating cell growth, adhesion, and motility. This modification is studied extensively in cancer research due to Src's association with tumor progression and metastasis. In B2B contexts, phosphorylated Src is supplied as a reagent for biochemical assays, drug discovery, and academic research. Suppliers often provide it in lyophilized form or pre-diluted solutions, accompanied by certificates of analysis detailing purity and phosphorylation levels.
Physical and Chemical Properties
Phosphorylated Src is a protein with a molecular weight of approximately 60-65 kDa, depending on the isoform. It exhibits solubility in standard aqueous buffers like PBS or Tris-HCl, making it suitable for in vitro experiments. The phosphorylated form is structurally distinct due to the added phosphate group, which alters its conformation and binding affinity. Stability is a key consideration; p-Src degrades under repeated freeze-thaw cycles or prolonged exposure to room temperature. Suppliers typically recommend storage at -20°C or lower, with aliquoting to preserve activity. Analytical techniques such as mass spectrometry or Western blotting are used to confirm phosphorylation status and purity.
Main Applications
Phosphorylated Src is pivotal in studying tyrosine kinase signaling networks, particularly in oncology. Researchers use it to investigate mechanisms of Src activation in cancers like colorectal, breast, and pancreatic tumors. It also serves as a positive control in kinase activity assays and high-throughput drug screening. Beyond cancer, p-Src is employed in cardiovascular and neurological research, where Src kinases modulate processes like angiogenesis and synaptic plasticity. Commercial kits for detecting phosphorylated proteins often include p-Src as a reference standard due to its well-characterized activity.
Safety and Storage
While phosphorylated Src is not classified as highly hazardous, standard laboratory precautions apply. Use personal protective equipment (PPE) such as gloves and lab coats to prevent skin contact. Avoid generating aerosols during reconstitution. Proper storage is critical: maintain lyophilized powder at -20°C and reconstituted solutions at -80°C for long-term stability. Aliquot the protein to minimize freeze-thaw cycles, which can degrade phosphorylation. Suppliers often include stabilizers like glycerol or BSA to prolong shelf life.
B2B Procurement Guide
When procuring phosphorylated Src, prioritize suppliers with documented quality controls, such as ISO certification or GMP compliance. Key specifications include purity (>90%), phosphorylation validation (e.g., by anti-phosphotyrosine antibodies), and endotoxin levels (<1 EU/μg for cell-based assays). Bulk pricing tiers are often available for high-volume buyers, but confirm batch-to-batch consistency. Lead times may vary; some vendors offer custom phosphorylation services for specific research needs. Consider logistical factors like cold-chain shipping to preserve protein integrity.
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