Overview
Phosphofructokinase 6 (PFK-6) is a rate-limiting enzyme in the glycolytic pathway, critical for cellular energy production. It catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate, a key step regulated by allosteric effectors like ATP and AMP. Primarily sourced from microbial or mammalian systems, recombinant PFK-6 is widely used in biochemical research and industrial applications. Its role in metabolic flux makes it a target for drug development and biotechnology tools.
Physical and Chemical Properties
PFK-6 typically exists as a multimeric protein with subunits ranging from 30-40 kDa each. The enzyme is sensitive to pH (optimal activity at pH 7.5-8.5) and temperature (inactivated above 50°C). Its activity depends on Mg²⁺ as a cofactor and is modulated by energy metabolites. Commercial preparations often include stabilizers like glycerol or salts to prolong shelf life. Lyophilized forms retain activity for years when stored properly.
Main Applications
In biotechnology, PFK-6 is utilized to study glycolysis inhibitors for cancer therapy or antimicrobial development. It’s also integrated into glucose metabolism assays for clinical diagnostics. Pharmaceutical industries employ PFK-6 in enzyme-coupled systems for high-throughput screening of metabolic drugs. Recent advances explore its use in synthetic biology for optimizing biofuel production pathways.
Safety and Storage
While PFK-6 is not classified as hazardous, proper lab practices are essential. Avoid inhalation of lyophilized powder and use gloves when handling solutions. Spills should be neutralized with water and cleaned promptly. Long-term storage requires airtight containers at -80°C with desiccants. For frequent use, aliquot solutions to minimize freeze-thaw degradation. Activity assays should confirm stability post-thawing.
B2B Procurement Guide
Bulk buyers should prioritize suppliers with ISO 13485 certification for consistent quality. Key specifications include specific activity (≥50 units/mg protein), endotoxin levels (<0.1 EU/µg), and absence of proteases. Request batch-specific CoA and consider trial quantities for validation. Lead times for custom recombinant variants may extend to 8-12 weeks. Negotiate cold-chain logistics for international shipments.
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