Overview
Phenanthroline Indicator, chemically known as 1,10-Phenanthroline, is a heterocyclic organic compound widely used in analytical chemistry. It serves as a chelating agent, forming stable complexes with metal ions, particularly iron (Fe²⁺ and Fe³⁺). The compound is valued for its ability to produce distinct color changes in the presence of specific metal ions, making it indispensable in redox titrations and spectrophotometric analyses. Its role in detecting iron concentrations in water, pharmaceuticals, and industrial samples has established it as a critical reagent in environmental and quality control laboratories. The compound's stability and sensitivity to low metal ion concentrations further enhance its utility in analytical procedures.
Physical and Chemical Properties
Phenanthroline Indicator is a white to pale yellow crystalline powder with a molecular weight of 180.21 g/mol. It has a melting point range of 117-120 °C and is soluble in polar solvents such as water, ethanol, and methanol. The compound's density is approximately 1.31 g/cm³, and it exhibits strong chelating properties due to its nitrogen-containing heterocyclic structure. When complexed with iron ions, Phenanthroline forms a distinctive orange-red color, which is the basis for its use in colorimetric assays. The compound's stability under standard laboratory conditions and its ability to form stable complexes with divalent and trivalent metal ions make it a versatile tool in analytical chemistry.
Main Applications
Phenanthroline Indicator is primarily used in analytical chemistry for the detection and quantification of iron ions. It is a key component in the phenanthroline method, a standard procedure for measuring iron concentrations in water, biological fluids, and industrial samples. The compound's ability to form colored complexes with iron allows for precise spectrophotometric analysis. Beyond iron detection, Phenanthroline is employed in redox titrations and as a chelating agent in various chemical processes. Its applications extend to environmental monitoring, pharmaceutical quality control, and research laboratories where metal ion detection is critical. The compound's specificity and sensitivity make it a preferred choice for many analytical protocols.
Safety and Storage
Phenanthroline Indicator is classified as harmful if swallowed and can cause skin and eye irritation. Proper personal protective equipment (PPE), including gloves and safety goggles, should be worn when handling the compound. In case of contact, rinse affected areas with plenty of water and seek medical advice if irritation persists. Storage conditions are critical to maintaining the compound's stability. Phenanthroline should be kept in a cool, dry place, away from direct light and moisture. Containers should be tightly sealed to prevent contamination and degradation. Proper labeling and adherence to safety data sheet (SDS) guidelines are essential for safe handling and storage in laboratory settings.
B2B Procurement Guide
When procuring Phenanthroline Indicator for B2B applications, buyers should prioritize purity levels (≥99%) to ensure accurate analytical results. Verifying the CAS number (66-71-7) is essential to avoid counterfeit or substandard products. Reputable suppliers often provide certificates of analysis (CoA) to confirm purity and quality. Bulk purchases may offer cost advantages, but storage capacity and shelf life should be considered. Buyers should also evaluate supplier reliability, delivery timelines, and compliance with regulatory standards. For specialized applications, custom formulations or packaging options may be available upon request. Always request samples for quality testing before large-scale procurement.
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