Overview
Phaseic acid standard is a certified reference material derived from the catabolism of abscisic acid (ABA) in plants. As a key metabolite in the ABA pathway, it serves as an essential calibration standard for analytical techniques quantifying plant hormone dynamics. Primarily utilized by plant physiology laboratories and agrochemical researchers, this standard enables precise measurement of phaseic acid concentrations in plant tissues during stress responses. Its standardized purity allows for reproducible results across hormone profiling studies.
Physical and Chemical Properties
Phaseic acid standard typically exhibits high crystallinity with a molecular weight of 280.32 g/mol. The compound demonstrates moderate polarity, requiring polar organic solvents for dissolution. Thermal analysis shows decomposition beginning near 180°C without distinct boiling. Chromatographic properties include characteristic retention times in reverse-phase HPLC (typically 8-12 minutes with C18 columns) and identifiable mass spectra fragments (major ions at m/z 279 [M-H]-, 261, 219). UV-Vis spectroscopy reveals maximum absorption around 260 nm in methanol solutions.
Main Applications
In plant science research, phaseic acid standards are indispensable for: 1) Quantifying ABA metabolic flux in drought/stress experiments, 2) Validating novel hormone extraction protocols, and 3) Calibrating LC-MS/MS systems for precision agriculture applications. The pharmaceutical industry employs these standards in botanical drug development, particularly for standardizing plant-derived formulations. Agricultural biotech firms use them to monitor transgenic plant responses during ABA-related trait development.
Safety and Storage
While phaseic acid presents low acute toxicity (LD50 >2000 mg/kg oral rats), proper laboratory handling with nitrile gloves and protective eyewear is recommended. Long-term stability requires storage at -20°C in argon-flushed amber vials with desiccant packs. For laboratory safety compliance, material safety data sheets (MSDS) should be maintained even though the compound is not classified as hazardous under GHS criteria. Degradation products from improper storage may exhibit different toxicity profiles.
B2B Procurement Guide
Research-grade phaseic acid standards should be sourced from specialized phytochemical suppliers with ISO 17025 accredited analytical capabilities. Key procurement considerations include: batch-specific certificates of analysis documenting purity (HPLC-UV/DAD ≥98%), absence of ABA contamination, and chromatographic reference data. For traceability, request NMR and HRMS characterization data when available. Bulk purchases (100mg+) may qualify for 15-30% discounts from major suppliers. Lead times for custom purification typically range 4-8 weeks.
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