Overview
Human P-Selectin (CD62P) is a transmembrane glycoprotein belonging to the selectin family, primarily expressed on activated platelets and endothelial cells. It plays a critical role in mediating the initial tethering and rolling of leukocytes during inflammatory responses. P-Selectin is stored in Weibel-Palade bodies (endothelial cells) and α-granules (platelets), rapidly translocating to the cell surface upon activation. The molecule’s extracellular domain includes a lectin domain, an epidermal growth factor (EGF)-like domain, and short consensus repeats (SCRs). Its ligand, PSGL-1, is expressed on leukocytes, enabling cross-cell adhesion. Research applications span thrombosis, atherosclerosis, and cancer metastasis studies.
Physical and Chemical Properties
P-Selectin is a type-I membrane protein with a molecular weight of approximately 140 kDa, heavily glycosylated. The protein’s stability is pH-dependent, with optimal activity in neutral buffers (pH 7.0–7.4). Recombinant forms (e.g., soluble P-Selectin) are commonly produced in mammalian expression systems to ensure proper post-translational modifications. Lyophilized preparations typically require reconstitution in sterile PBS or similar buffers. The protein is susceptible to degradation by proteases; thus, protease inhibitors are recommended in experimental setups. Dynamic light scattering (DLS) or size-exclusion chromatography (SEC) can confirm monomeric stability.
Main Applications
In biomedical research, P-Selectin is pivotal for studying inflammatory diseases, such as rheumatoid arthritis and sepsis. It serves as a biomarker for platelet activation in cardiovascular diseases. Drug developers target P-Selectin to design anti-inflammatory or antithrombotic therapies (e.g., P-Selectin inhibitors like crizanlizumab). Diagnostically, ELISA kits leverage P-Selectin antibodies to quantify its levels in plasma. In vitro flow chamber assays model leukocyte adhesion under shear stress, mimicking vascular conditions. Cancer research explores its role in tumor cell extravasation and metastasis.
Safety and Storage
P-Selectin is generally safe to handle but requires standard lab precautions. Recombinant forms should be treated under Biosafety Level 1 (BSL-1) protocols. Avoid inhalation or direct contact with powders; use gloves and goggles. For storage, lyophilized protein is stable at -20°C for 1–2 years; solutions should be aliquoted and stored at -80°C to prevent activity loss. Repeated freeze-thaw cycles degrade functionality. Include carrier proteins (e.g., BSA) in dilute solutions to prevent surface adsorption.
B2B Procurement Guide
When sourcing Human P-Selectin, prioritize suppliers with ISO 13485 or GMP certification for consistent quality. Key specifications include ≥90% purity (SDS-PAGE/Coomassie), low endotoxin levels (<1 EU/μg), and validated biological activity (e.g., IC50 in binding assays). Bulk orders (10+ mg) may qualify for discounts; request batch-specific CoA. Compare vendors like R&D Systems, Sino Biological, or Abcam for competitive pricing. For diagnostic or therapeutic use, ensure regulatory compliance (e.g., FDA/CE-marked reagents). Lead times vary; plan for 2–4 weeks for custom recombinant production.
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