Overview
Optical Bottom Microplates are precision-engineered for laboratories requiring high-sensitivity optical measurements. Their ultra-thin, transparent bottoms minimize light scattering and background noise, critical for fluorescence resonance energy transfer (FRET) and other advanced assays. Manufacturers often use polymer materials like COC for superior clarity and thermal stability. These plates are indispensable in pharmaceutical R&D, where they facilitate kinetic studies and endpoint detection with minimal variability between wells.
Structure and Working Principle
The microplate consists of an array of wells (typically 6 to 1536) fused to an optically clear base, usually ≤1 mm thick. The microporous design allows gas exchange for cell-based assays while maintaining sterility. Advanced variants incorporate black walls to reduce cross-talk between wells during fluorescence readings. Some models feature skirted designs for robotic handling, with barcoding options for traceability in automated workflows.
Key Features
Low autofluorescence is achieved through stringent polymer purification, crucial for sensitive GFP or luciferase reporter assays. Plates may include surface treatments like tissue-culture coating or non-binding coatings for specific applications. Temperature tolerance ranges from -80°C to 121°C (autoclavable grades), accommodating diverse experimental conditions. Leading brands offer ISO 9001-certified production to ensure batch-to-batch consistency in optical properties.
Application Areas
Primary applications include ELISA, cell viability assays, and nucleic acid quantification. In drug discovery, they enable high-content screening (HCS) with confocal microscopy compatibility. Academic labs utilize them for protein crystallization studies, while diagnostic manufacturers employ them in automated immunoassay systems. Emerging uses include organ-on-chip platforms requiring real-time imaging.
Maintenance and Precautions
Clean with mild detergent; avoid abrasive tools that could scratch optical surfaces. Store in dust-free environments to prevent particulate interference during measurements. For cell culture applications, validate plate sterility and gas permeability. Thermal cycling between extreme temperatures may require plates with enhanced polymer formulations to prevent warping.
B2B Procurement Guide
Bulk buyers should request certified test data for optical clarity (typically >90% transmission at 400–700 nm). Evaluate supplier capabilities for custom configurations like specialized well geometries or integrated membrane layers. Consider total cost of ownership: some premium plates reduce reagent consumption through smaller well volumes, offsetting higher unit costs. Lead times for specialty orders may extend to 8–12 weeks.
Related Manufacturers
- 主营：滤膜、滤纸、滤器、微孔板、超滤管
- 主营：真空泵、八连管、滤芯吸头、康宁96孔微孔板、原位杂交仪、转印芯模块、灭菌加长吸头
- 主营：透明板、培养板、细菌培养皿
- 主营：过滤板
- 主营：计数池、检测仪、流通池、光学检测光源、光谱仪、流动池、鞘流池、闪烁氙灯、光源配件、适配定制检测池、监测气体分析仪
- 主营：离心管、聚酯膜、培养瓶、处理微孔板、超低吸附微孔板、细胞培养板、聚碳酸酯膜、外旋冻存管、内旋冻存管、细胞培养皿
- 主营：防排水板、蓄排水板、虹吸排水系统、pp模块雨水、植草格、排水沟、土工膜、土工布、防水毯、盲沟
- 主营：过滤机、脱水机、过滤设备、陶瓷滤膜、陶瓷过滤机、脱水设备、过滤材料、陶瓷过滤板、固液分离设备、陶瓷板清洗剂、陶瓷过滤机配件、带式真空过滤机、压滤机、潜孔钻机、150潜孔钻机
- 主营：冲孔网、冲孔板、不锈钢冲孔网、钢板网、菱形钢板网、冲孔板网、金属网、走道板、盾构走道板、隧道走道板、金属网片、金属板、多孔板、装饰网、冲孔钢板网、冲孔过滤板、冲孔金属网、冲孔装饰网、微孔冲孔板、地铁隧道走道板、热镀锌走道板、防滑脚踏板、脚踏板、镀锌冲压走道板
- 主营：微孔陶瓷过滤砖、微孔陶瓷过滤板、微孔陶瓷过滤管、氨水过滤器、耐酸瓷砖、稀土瓷砂、陶瓷波纹填料、陶瓷鲍尔环、蜂窝陶瓷蓄热体、陶瓷拉西环、耐酸耐温砖、空心浮球、陶瓷波纹板、陶瓷膜过滤器、耐酸瓷管
- 主营：陶瓷过滤机、陶瓷过滤板、陶瓷板清洗剂、带式真空过滤机、浓缩机、浓密机、潜孔钻机、陶瓷过滤机配件、陶瓷板、过滤机、150潜孔钻机