Overview
Neuropathy Target Esterase (NTE) is a membrane-associated enzyme predominantly expressed in neural tissues. Initially identified as the molecular target of organophosphate compounds causing delayed neuropathy, it plays a vital role in phospholipid metabolism. The enzyme's inhibition leads to axonal degeneration, making it a key subject in neurotoxicology studies. NTE belongs to the patatin-like phospholipase family and exhibits lysophospholipase activity. Its physiological function involves maintaining neuronal membrane integrity, though the exact mechanisms remain under investigation. The enzyme's structure includes a regulatory domain and catalytic serine esterase domain, the latter being the site of organophosphate binding.
Physical and Chemical Properties
As a protein, NTE's properties differ from small-molecule chemicals. It has a molecular weight of approximately 155 kDa and requires membrane association for full activity. The enzyme operates optimally at physiological pH and temperature, with its activity measurable through specialized esterase assays using substrates like phenyl valerate. NTE demonstrates sensitivity to organophosphate inhibitors, which form irreversible bonds with its active site serine residue. This inhibition property is exploited in laboratory settings to study neuropathic effects. The enzyme shows tissue-specific isoforms, with neural variants having distinct kinetic parameters compared to those in non-neural tissues.
Main Applications
In industrial toxicology, NTE serves as a biomarker for assessing the neuropathic potential of organophosphate compounds. Pesticide manufacturers routinely test new formulations for NTE inhibition rates to predict OPIDN risk. Regulatory agencies use NTE inhibition thresholds (typically >70%) as safety benchmarks. Research applications include studying neurodegenerative mechanisms and developing neuroprotective strategies. Some pharmaceutical investigations target NTE modulation for treating lipid metabolism disorders. In clinical settings, NTE activity measurements aid in diagnosing organophosphate poisoning cases with delayed neuropathic symptoms.
Safety and Storage
While NTE itself isn't hazardous, associated organophosphate inhibitors are highly toxic. Laboratories working with these compounds must follow strict biosafety protocols, including fume hood use, chemical-resistant gloves, and emergency shower access. Contaminated materials require specialized disposal as hazardous waste. Purified NTE proteins for research require storage at -20°C in glycerol-containing buffers to prevent denaturation. Lyophilized forms may have longer shelf lives but need reconstitution verification. Shipping typically requires dry ice for stability maintenance, with temperature monitoring during transport.
B2B Procurement Guide
Research-grade NTE is available from specialized biochemical suppliers, typically as recombinant human or animal-derived forms. Key specifications include activity units (µmol/min/mg protein), purity level (SDS-PAGE verified), and whether it's membrane-bound or solubilized. Some vendors provide inhibition assay kits with control organophosphates. For industrial testing purposes, validated NTE sources with certification for regulatory compliance are essential. Bulk purchases may negotiate pricing, but quality validation through third-party testing is recommended. Lead times can extend to 4-6 weeks for custom preparations. Always request material safety data sheets for all related compounds.
Related Manufacturers
- 主营：科研试剂盒、酶免试剂盒、elisa试剂盒、生化试剂盒、细胞、生物试剂、进口ELISA试剂盒、PCR试剂盒、生化测试盒
- 主营：ELISA试剂盒、高灵敏度试剂盒、试剂盒厂家、人ELISA试剂盒、小鼠ELISA试剂盒、大鼠ELISA试剂盒、仓鼠ELISA试剂盒、豚鼠ELISA试剂盒、鸡ELISA试剂盒、鸭ELISA试剂盒、牛ELISA试剂盒、羊ELISA试剂盒、马ELISA试剂盒、猴ELISA试剂盒、猪ELISA试剂盒、犬ELISA试剂盒、兔ELISA试剂盒、鱼ELISA试剂盒、植物ELISA试剂盒、土壤ELISA试剂盒、定性ELISA试剂盒、肿瘤标志物检测ELISA试剂盒、特种蛋白检测ELISA试剂盒、传染病检测ELISA试剂盒
- 主营：ELISA试剂盒、PCR试剂盒、科研试剂
- 主营：试剂盒、ELISA试剂盒、科研ELISA试剂盒、PCR试剂盒、生化试剂盒
- 主营：试剂盒、辅激活因子、elisa试剂盒、鸡神经疾病靶标酯酶、蛋白、多肽、金标试剂盒、PCR试剂盒、抗生素、氨基酸、血清、抗体
- 主营：ELISA试剂盒、生化检测试剂盒、PCR试剂盒、大鼠ELISA试剂盒、小鼠ELISA试剂盒、兔ELISA试剂盒、鸡ELISA试剂盒
- 主营：scl-70igg、人1α羟、试剂盒、大鼠神经疾病靶标酯酶、葡聚糖、大鼠11β、16α羟基、1iga抗体、胶原c端、2-he2elisa、1igm抗体、25二羟基、17羟孕酮、dna糖苷酶、5α还原酶、研启生物、去氢血栓、人胰岛素、胶原n末端、大鼠8羟基、25羟基维生、2，5寡腺苷、大鼠25羟基、11脱氢血栓、羟基壬烯醛、三磷酸肌醇
- 主营：试剂盒、ELISA试剂盒、PCR试剂盒、科研细胞系
- 主营：试剂盒、ELISA试剂盒、ELISA检测试剂盒
- 主营：大鼠神经疾病靶标酯酶、试剂盒
- 主营：大鼠神经疾病靶标酯酶、检测试剂盒
- 主营：试剂盒、同源物、脂蛋白、合蛋白、粘液素、抑制素、亲环素、保护素、信号素、胞色素、脂肪酶、末端肽、磷脂酶、蛋白酶、变位酶、抑制剂、脱水酶、抑制物、凝蛋白、激活肽、连接酶、样激酶、解氨酶、恒温箱、利钠肽
- 主营：神经疾病靶标酯酶、elisa试剂盒
- 主营：elisa试剂盒、生化试剂盒、pcr检测试剂盒、神经疾病靶标酯酶、细胞
- 主营：Elisa试剂盒、生化试剂盒、Pcr试剂盒、神经疾病靶标酯酶、细胞、抗体