Overview
Mid-carboxylated osteocalcin (N-MID Osteocalcin) is a stable fragment of the bone-specific protein osteocalcin, produced during bone formation. It serves as a sensitive biomarker for bone turnover, particularly in osteoporosis diagnosis and treatment monitoring. The N-MID fragment exhibits greater stability in serum samples compared to intact osteocalcin, making it preferable for clinical assays. First identified in the 1980s, this peptide fragment contains amino acids 1-43 of the full osteocalcin molecule, retaining the N-terminal and mid-region structures critical for antibody recognition in diagnostic tests. Its measurement has become standard in metabolic bone disease assessment.
Physical and Chemical Properties
As a protein fragment, N-MID osteocalcin maintains tertiary structure through disulfide bonds and post-translational modifications, including gamma-carboxylation of glutamic acid residues. This modification requires vitamin K-dependent enzymes and enhances calcium-binding capacity in bone tissue. The fragment demonstrates stability in serum at room temperature for up to 24 hours and at 4°C for several days, though long-term storage requires freezing. It remains immunoreactive after multiple freeze-thaw cycles when properly preserved with protein stabilizers. Analytical methods typically employ monoclonal antibodies targeting the stable N-MID epitope.
Main Applications
In clinical diagnostics, N-MID osteocalcin measurement helps evaluate bone formation activity in osteoporosis, Paget's disease, and renal osteodystrophy. It complements other markers like CTX (C-terminal telopeptide) to assess bone turnover balance. The test proves particularly valuable for monitoring antiresorptive therapy effectiveness in postmenopausal women. Research applications include studying bone metabolism mechanisms, vitamin K deficiency impacts, and drug development for metabolic bone disorders. Some studies suggest potential roles in energy metabolism and male fertility regulation, though these require further investigation.
Safety and Storage
As a biological reagent, N-MID osteocalcin requires standard biosafety level 1 handling with basic personal protective equipment. The lyophilized form remains stable for years at -20°C when kept desiccated, while reconstituted solutions should be aliquoted to avoid contamination. Transport typically requires cold chain maintenance with gel packs or dry ice for international shipments. Laboratories should validate stability under their specific storage conditions, as aggregation may occur in improperly handled samples, potentially affecting assay performance.
B2B Procurement Guide
When sourcing N-MID osteocalcin for commercial or research use, verify the supplier's quality certifications (ISO 13485 for diagnostic applications). Key specifications include immunoreactivity (>95% by ELISA), endotoxin levels (<1 EU/μg), and batch-to-batch consistency. For diagnostic kit manufacturers, request detailed cross-reactivity data against related peptides. Bulk purchasers should negotiate stability guarantees and consider dual sourcing for critical applications. Lead times for custom quantities typically range 4-8 weeks, with MOQs starting at 100mg for research-grade material.
Related Manufacturers
- 主营：人N端中段骨钙素ELISAKit、ELISA试剂盒、PCR试剂盒、科研试剂
- 主营：elisa 试剂盒、Elisa试剂盒、进口试剂盒、人ELISA试剂盒、国产试剂盒、试剂、细胞、ELISA检测试剂盒、酶联免疫法、酶联免疫试剂盒、酶活性检测、酶标仪检测
- 主营：酶cγ链、α2巨球、素d合成、牛骨钙素、纤连蛋白、抗组蛋白、肌醇抗体、蛋白样环、敏感蛋白、蛋白重链、金属肽酶、乳球蛋白、钩端螺旋、蛋白激酶、高血糖素、钙调蛋白、小鼠α1抗、ELISA试剂盒、pcr检测试剂、生化检测试剂
- 主营：人elisa试剂盒、ELISA试剂盒、抗体
- 主营：人N端中段骨钙素、试剂盒
- 主营：试剂盒、ELISA试剂盒、科研ELISA试剂盒、PCR试剂盒、生化试剂盒
- 主营：ELISA、分光光度法、微量法、人试剂盒、人ELISA试剂盒、ELISA试剂盒、大鼠试剂盒、小鼠试剂盒、植物试剂盒、酶联免疫法、MEGAZYME、大鼠ELISA试剂盒、小鼠ELISA试剂盒、植物ELISA 试剂盒、白介素试剂盒、细胞因子试剂盒、激素试剂盒、科研试剂盒、化学试剂、检测试剂盒、土壤试剂盒、土壤酶活性检测
- 主营：人N端中段骨钙素ELISA、ELISA试剂盒、PCR试剂盒、科研试剂
- 主营：N端中段骨钙素、ELISA试剂盒、PCR检测试剂盒、抗体
- 主营：elisa试剂盒、白细胞介素试剂盒、肿瘤坏死因子试剂盒、人N端中段骨钙素、植物检测试剂盒
- 主营：ELISA试剂盒、PCV试剂盒、生化试剂盒、分子生物试剂盒、常规生化试剂盒、ATCC细胞、USP标准品、血清、抗体
- 主营：人乙酰胆碱酯酶、特异性抗原、带状疱疹病毒、脂肪酸结合蛋白
- 主营：elisa试剂盒、检测试剂盒、酶免试剂盒、通蔚生物试剂盒、elisakit
- 主营：试剂盒、同源物、脂蛋白、人N端中段骨钙素、合蛋白、粘液素、抑制素、亲环素、保护素、信号素、胞色素、脂肪酶、末端肽、磷脂酶、蛋白酶、变位酶、抑制剂、脱水酶、抑制物、凝蛋白、激活肽、连接酶、样激酶、解氨酶、恒温箱、利钠肽
- 主营：布病抗体快速检测卡、猪瘟病毒抗体快速检测卡、口蹄疫抗体快速检测卡、人N端中段骨钙素、小反刍抗体快速检测卡、猪蓝耳病抗体快速检测卡、牛早孕胶体金快速检测卡、禽流感抗体快速检测卡、狂犬病毒抗体快速检测卡