Overview
Mycoplasma Broth is a specialized liquid culture medium formulated to support the growth of Mycoplasma species, which are unique bacteria that lack cell walls and have complex nutritional requirements. Developed specifically for these fastidious microorganisms, the medium typically contains beef heart infusion, peptones, yeast extract, and animal serum (often horse or fetal bovine serum) to provide essential nutrients, cholesterol, and fatty acids. This medium serves critical roles in both research and quality control environments. In pharmaceutical manufacturing, it's used to test for Mycoplasma contamination in cell cultures and biological products. Clinical laboratories employ it for isolating Mycoplasma pathogens from patient specimens, particularly in respiratory and urogenital infections.
Physical and Chemical Properties
Mycoplasma Broth appears as a clear to slightly opaque liquid with an amber or straw color, depending on its specific formulation. The pH is carefully adjusted to approximately 7.2-7.4 to optimize Mycoplasma growth. The medium's osmolarity typically ranges between 280-320 mOsm/kg to match physiological conditions. Key components include enzymatic digests of protein (peptones) which provide nitrogen sources and amino acids, yeast extract supplying B vitamins, and serum that delivers essential lipids and growth factors. Many formulations incorporate phenol red as a pH indicator, changing from red-orange to yellow if acid production occurs during bacterial metabolism. The broth remains stable when stored properly but should never be frozen as this may precipitate serum components.
Main Applications
The primary application of Mycoplasma Broth is in the detection and cultivation of Mycoplasma species in various settings. In pharmaceutical quality control, it's mandated by regulatory agencies for testing cell banks and biological products for Mycoplasma contamination. The broth serves as both a direct growth medium and as part of indicator cell culture methods. Clinical microbiology laboratories use this medium to isolate pathogenic Mycoplasma species from patient samples, particularly Mycoplasma pneumoniae in respiratory infections and Mycoplasma hominis or Ureaplasma species in genitourinary specimens. Veterinary diagnostics similarly employ the broth for detecting Mycoplasma infections in livestock and poultry, which can cause significant economic losses in agriculture.
Safety and Storage
While Mycoplasma Broth itself is non-toxic, proper handling precautions are essential as cultured specimens may contain pathogenic organisms. Work should be conducted in biosafety cabinets when processing potentially infectious material. Used media must be decontaminated by autoclaving before disposal. For storage, maintain the broth at 2-8°C in airtight containers protected from light. Avoid repeated warming and cooling cycles. Once opened or prepared, use within the manufacturer's specified period (typically 1-4 weeks). Never use media that appears cloudy or contains precipitate unless specified by the formulation. Always check for intact seals and absence of microbial contamination before use.
B2B Procurement Guide
When procuring Mycoplasma Broth commercially, verify the supplier's quality certifications including ISO 13485 for medical devices or equivalent. Key specifications to confirm include: serum content percentage (commonly 10-20%), presence of antibiotics (if any), and included quality controls like sterility testing certificates. For large-scale users, consider ready-to-use liquid formulations versus powder media requiring preparation. Liquid media offer convenience but have shorter shelf lives. Evaluate supplier lead times as some serum-containing media may require longer production cycles. For specialized applications, some manufacturers offer custom formulations with adjusted serum levels or additional nutrients. Always request batch-specific certificates of analysis.
Related Manufacturers
- 主营：大肠杆菌、琼脂平皿、琼脂平板、tge肉汤、双歧杆菌、isp培养基、pcfa培养基、琼脂培养基、一次性试管、硫乙醇酸盐、生理盐水管、高压灭菌袋、korthof培养基、蛋白胨培养基、检定用培养基、无机铁磷培养基、中和增菌培养基
- 主营：大肠杆菌、琼脂培养基、磷酸盐缓冲液、7h9肉汤、肉汤管、胰蛋白胨胆盐
- 主营：试剂盒、增菌液、平板9cm、肉汤lb2、m17琼脂、化妆品、blb琼脂、培养皿、肠球菌、mrs琼脂、gam琼脂、bhi琼脂、培养基、厌氧产、营养琼脂、菌落计数、采样管、采样袋、水样采集袋、菌片、分离培养、琼脂平板、食品检验、药典、鉴定条
- 主营：蛋白酶、淀粉酶、果胶酶、胰酵素、崩溃酶、蜗牛酶、乳糖酶、肌酸激酶、葡球菌酶、绿色木霉、心肌黄酶、亮抑酶肽、纤维素酶、木聚糖酶、-glucosidase、亮抑肽酶、硫代辅酶、枯草杆菌、羧化辅酶、氯化酵素、脆壁质酶、细胞溶解酶、乙醇脱氢酶、透明质酸酶、氯化溶菌酶
- 主营：科研试剂、高效试剂
- 主营：研突破、转座酶、重组酶、连接酶、研试剂、(5×)(with、recombinant、结合蛋白、胶体染料、核酸染料、助力科研、大肠杆菌、泛素蛋白、实验室用品、重组肠激酶、电泳缓冲液、拓扑异构酶、热敏磷酸酶、检测试剂盒、全能核酸酶、核酸外切酶、核酸内切酶、连接试剂盒、碱性磷酸酶、微球菌核酸酶
- 主营：马铃薯、增菌液、噬菌体、PPLO肉汤基础、营养盐、串珠菌、琼脂粉、西红柿、活性炭、营养液、酵母氮、基础盐、血琼脂、酵母膏、磁螺菌、缓冲液、抑菌剂、培养基、氯化钠、玉米粉、三糖铁、洗脱液、中和剂、结冷胶、沉降菌、橄榄油
- 主营：感受态、扁桃体、淋巴管、肺炎支原体肉汤基础、干细胞、狗血浆、羊血浆、导管癌、原细胞、青霉素、营养液、无酚红、癌细胞、小细胞、胰腺癌、输尿管、抗凝鸡、缓冲液、鸭血清、猪血清、人韧带、培养基、肝素钠、牛血浆、马血清、白细胞
- 主营：骨细胞、腺嘌呤、微量法、PPLO肉汤、试剂盒、脂蛋白、提试剂、增菌液、测试盒、光度法、瘤细胞、纤维素、信号素、缺锌ynb、病毒探、肝细胞、饥饿素、粘蛋白、蛋白酶、混合液、半乳糖、抑制剂、激活肽、蛋白质、癌细胞、连接酶
- 主营：PPLO肉汤、试剂盒、ELISA试剂盒