Overview
Mouse Soluble E-selectin (sE-selectin) is a cleaved form of the transmembrane glycoprotein E-selectin, released during endothelial cell activation. It plays a critical role in leukocyte recruitment during inflammatory responses. As a research reagent, it is widely used to study vascular inflammation, atherosclerosis, and sepsis. In B2B contexts, sE-selectin is primarily supplied to pharmaceutical companies and academic laboratories. Commercial products are typically recombinant proteins produced in mammalian expression systems, ensuring proper glycosylation and biological activity.
Physical and Chemical Properties
Mouse sE-selectin is a glycoprotein with a molecular weight of approximately 110-140 kDa in its glycosylated form. The protein consists of an N-terminal lectin domain, an epidermal growth factor (EGF)-like domain, and multiple complement regulatory protein repeats. It retains ligand-binding capability to sialyl-Lewis X carbohydrates. The lyophilized form is stable at -20°C for years but requires reconstitution in sterile buffers (e.g., PBS) before use. In solution, it should be aliquoted to avoid degradation from repeated freeze-thaw cycles. Purity is typically >90% by SDS-PAGE, with endotoxin levels <1 EU/µg.
Main Applications
In biomedical research, mouse sE-selectin serves as a biomarker for endothelial activation in disease models such as rheumatoid arthritis, inflammatory bowel disease, and ischemia-reperfusion injury. It is also used to develop ELISA kits for quantifying circulating levels in preclinical studies. Pharmaceutical companies employ sE-selectin in drug screening assays to evaluate anti-inflammatory compounds targeting leukocyte-endothelial interactions. Additionally, it is utilized in flow cytometry and cell adhesion assays to investigate mechanistic pathways in vascular biology.
Safety and Storage
Mouse sE-selectin is classified as non-hazardous under normal laboratory conditions. Standard biosafety level 1 (BSL-1) precautions apply, including gloves and lab coats. Avoid inhalation or direct contact with skin/eyes. For storage, lyophilized powder should be kept at -20°C in a desiccator. Reconstituted solutions are stable for up to 1 week at 4°C or 3 months at -80°C. Always centrifuge vials briefly before opening to prevent protein loss. Suppliers typically provide detailed handling instructions with certificates of analysis (CoA).
B2B Procurement Guide
When procuring mouse sE-selectin, prioritize suppliers with ISO 9001 certification and documented quality controls. Key specifications to verify include: biological activity (e.g., binding affinity in SPR assays), endotoxin levels, and batch-to-batch consistency. Bulk purchasers (e.g., CROs) should negotiate volume discounts for orders exceeding 1 mg. Consider requesting custom formulations (e.g., carrier-free options) for specific assay requirements. Lead times for GMP-grade material may extend to 8-12 weeks. Always review SDS and technical data sheets before purchase.
Related Manufacturers
- 主营：selectinELISAKit、ELISA试剂盒、PCR试剂盒、科研试剂
- 主营：科研试剂盒、酶免试剂盒、细胞、elisa试剂盒、生物试剂、进口ELISA试剂盒、PCR试剂盒、生化测试盒
- 主营：检测方、钠尿肽、试剂盒、ep300检测、大鼠e选择素、elisa 检测试剂盒、同源物、结合蛋白、大鼠c-肽(、微球蛋白、抗胰蛋白酶、淋巴瘤因子、蛋白磷酸酶、死亡激动剂、核心域蛋白1、大鼠janus激酶、激活蛋白激酶、大鼠ⅱ型胶原、大鼠ⅳ型胶原、大鼠klotho蛋白、细胞激活因子、大鼠ⅲ型胶原、大鼠nadh脱氢酶1、大鼠ⅵ型胶原α1
- 主营：小鼠可溶性E选择素、检测试剂盒
- 主营：小鼠可溶性E选择素、试剂盒
- 主营：ELISA试剂盒、PCR试剂盒、生化试剂盒、selectin、科研试剂
- 主营：小鼠可溶性E选择素、ATCC细胞系、原代细胞、原代永生化
- 主营：elisa试剂盒、试剂盒、抗体
- 主营：scl-70igg、人1α羟、试剂盒、2-he2elisa、葡聚糖、大鼠11β、16α羟基、1iga抗体、胶原c端、1igm抗体、25二羟基、17羟孕酮、dna糖苷酶、5α还原酶、研启生物、去氢血栓、人胰岛素、胶原n末端、大鼠8羟基、25羟基维生、2，5寡腺苷、大鼠25羟基、11脱氢血栓、羟基壬烯醛、三磷酸肌醇
- 主营：elisa试剂盒
- 主营：生化试剂盒、PCR试剂盒、检测试剂盒、elisa试剂盒、科研试剂盒
- 主营：elisa 试剂盒
- 主营：试剂盒、同源物、脂蛋白、小鼠可溶性E选择素、合蛋白、粘液素、抑制素、亲环素、保护素、信号素、胞色素、脂肪酶、末端肽、磷脂酶、蛋白酶、变位酶、抑制剂、脱水酶、抑制物、凝蛋白、激活肽、连接酶、样激酶、解氨酶、恒温箱、利钠肽
- 主营：elisa试剂盒、白细胞介素试剂盒、肿瘤坏死因子试剂盒、植物检测试剂盒
- 主营：elisa试剂盒、检测试剂盒