Overview
Mouse Podoplanin (PDPN) is a conserved O-glycosylated protein expressed on lymphatic endothelial cells, podocytes, and certain cancer cells. It plays critical roles in embryonic development, immune regulation, and pathological processes like tumor metastasis. The protein's extracellular domain contains platelet-aggregating regions that interact with CLEC-2 receptors. First identified in lung alveolar cells, Podoplanin has become a key target in biomedical research due to its unique involvement in lymphatic system formation. Commercial preparations are typically recombinant proteins produced in mammalian expression systems, ensuring proper glycosylation patterns for functional studies.
Physical and Chemical Properties
Mouse Podoplanin is a type-I transmembrane protein with a molecular weight of 36-40 kDa when glycosylated. The protein contains multiple serine/threonine-rich domains that undergo extensive O-glycosylation, critical for its biological activity. It maintains stability in pH 6-8 buffers but may degrade under repeated freeze-thaw cycles. Biochemical assays show high affinity for CLEC-2 (Kd ~10-100 nM depending on glycosylation state). The protein exhibits heat sensitivity, losing activity above 60°C. Dynamic light scattering analyses indicate a hydrodynamic radius of approximately 5-7 nm in solution, consistent with its elongated mucin-like structure.
Main Applications
In cancer research, Mouse Podoplanin serves as a marker for tumor-associated lymphatic vessels and facilitates studies on metastasis mechanisms. Researchers use it to investigate how tumors exploit lymphatic pathways for dissemination. The protein's platelet-activating properties make it valuable for modeling thromboembolic events in cardiovascular studies. Developmental biologists employ Podoplanin to track lymphatic endothelial cell differentiation. Recent applications include immuno-oncology research, particularly in checkpoint inhibitor studies where lymphatic vessel density correlates with treatment response. Some therapeutic antibodies are being developed against its extracellular domains.
Safety and Storage
Mouse Podoplanin requires standard biosafety level 1 handling precautions. While not classified as hazardous, users should avoid direct skin contact and aerosol formation. Lyophilized proteins remain stable for 2-3 years at -20°C when desiccated, while liquid formulations typically have 6-12 month shelf lives at 4°C with carrier proteins like BSA. For long-term preservation, aliquoting and storage at -80°C is recommended. Activity loss occurs if solutions are left at room temperature >24 hours. SDS-PAGE analysis should show a single band at ~40 kDa; degradation appears as smearing below this weight due to glycosylation heterogeneity.
B2B Procurement Guide
When sourcing Mouse Podoplanin, prioritize suppliers providing detailed glycosylation profiles and CLEC-2 binding validation data. Research-grade proteins should have ≥90% purity (HPLC-verified) and endotoxin levels <1 EU/μg. Bulk orders (10+ mg) often receive 15-30% discounts from specialty biotech vendors. Key procurement considerations include batch-to-batch consistency certificates and availability of matched negative controls. Some suppliers offer fluorescently labeled variants (e.g., FITC-conjugated) for imaging applications. Lead times range from 2-6 weeks for custom recombinant expressions, while off-the-shelf products typically ship within 5 business days.
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