Overview
Mouse Noggin is a secreted glycoprotein first identified for its role in embryonic head development. As a BMP antagonist, it selectively binds and inhibits BMP-2, -4, and -7, crucial for neural tube patterning and somite formation. The protein consists of 232 amino acids with a conserved cysteine knot domain. In research, recombinant Mouse Noggin is produced in E. coli or mammalian expression systems. Its purity (>95% by SDS-PAGE) and activity are critical for experimental reproducibility, particularly in stem cell differentiation protocols where it promotes neural progenitor formation.
Physical and Chemical Properties
Mouse Noggin typically migrates at 26-28 kDa on reducing SDS-PAGE due to glycosylation, though its theoretical molecular weight is ~22 kDa. The protein forms homodimers stabilized by disulfide bonds, essential for BMP binding. Lyophilized formulations are stable for years at -20°C but require reconstitution in acidic buffers (pH 4-5) to prevent aggregation. Biophysical studies show Noggin’s high affinity for BMPs (Kd ~1-10 nM). Its isoelectric point (pI) is approximately 8.5, influencing purification by ion-exchange chromatography. Researchers should note batch-to-batch variability in post-translational modifications between prokaryotic and eukaryotic expression systems.
Main Applications
In developmental biology, Mouse Noggin is used to study neural induction, where it suppresses BMP signaling to allow ectoderm differentiation into neural tissue. It’s a staple in cerebral organoid protocols, often combined with SMAD inhibitors. The protein also modulates osteogenesis—low doses promote cartilage formation by partial BMP inhibition, while complete inhibition blocks bone development. Pharmaceutical research explores Noggin’s potential in treating BMP-related pathologies like fibrodysplasia ossificans progressiva (FOP).
Safety and Storage
Recombinant Mouse Noggin is generally non-toxic at research concentrations (typically 50-200 ng/mL in cell culture). However, endotoxin contamination from production can affect sensitive primary cells—always specify <0.1 EU/μg for neural studies. For long-term storage, aliquot solutions in PBS with carrier proteins (e.g., 0.1% BSA) to prevent adsorption. Avoid repeated freeze-thaw cycles; lyophilized powder is stable for 3+ years at -20°C when desiccated. Sterile filtration (0.22 μm) is recommended for cell culture applications.
B2B Procurement Guide
When sourcing Mouse Noggin, prioritize vendors providing: 1) Certificate of Analysis with activity data (e.g., IC50 for BMP-4 inhibition), 2) Mass spectrometry verification of sequence, and 3) endotoxin reports. Large-scale buyers (mg quantities) should negotiate batch consistency clauses. Pricing varies by expression system—E. coli-derived Noggin (~$200/μg) is cheaper but lacks glycosylation, while mammalian-cell versions (~$400+/μg) better mimic native protein. Consider hybrid procurement strategies: purchase small test quantities from multiple suppliers before bulk orders.
Related Manufacturers
- 主营：增氧剂、消毒液、除雪剂、鱼蛋白、处理剂、消毒剂、硫酸铵、aes洗涤、润湿剂、催化剂、漂白粉、冲施肥、亚硝酸、尿素粉、全水溶、硫酸钠、氧化剂、防霉剂、清洗剂、漂白剂、片水产、洗涤剂、尿素桶、熟石灰、碳酰胺、碳酸钠
- 主营：卡拉胶、乳胶漆、聚乙烯、消泡剂、固化剂、分散剂、苯丙乳液、多环颜料、酸催化剂、颜料助剂、水性涂料、涂料助剂、乳品饮料、聚醛树脂、酞青颜料、水性木漆、烤漆助剂、果冻酱料、无机颜料、防腐涂料、高性能颜料、有机膨润土、糖果巧克力、涂料添加剂、水性润湿剂
- 主营：[]
- 主营：牛骨胶、胶黏剂、工业明胶、电镀光亮剂、提炼胶颗粒、增稠高粘度骨胶
- 主营：工业骨胶、工业明胶、牛胶、工业蛋白、蛋白粉、骨胶粉、明胶、皮胶、骨胶、牛胶粉、工业胶、水胶、牛骨胶、小颗粒胶、动物胶
- 主营：胶黏剂、牛皮胶、工业明胶、工业骨胶、胶凝剂颗粒、增稠剂颗粒、木工粘合剂、礼盒粘合胶、木材粘合用胶、冶炼电解牛骨胶、粘性稳定牛骨胶、增稠砂轮用骨胶、墨汁用的牛骨胶、砂轮板材粘合剂、明胶木板胶粘剂
- 主营：Elisa试剂盒、检测试剂盒、人elisa试剂盒、头蛋白、鼠elisa试剂盒、PCR检测试剂盒、生物试剂盒
- 主营：小鼠头蛋白、试剂盒、信号调节激酶、人转谷氨酰胺酶
- 主营：ELISA试剂盒、生化检测试剂盒、PCR试剂盒、小鼠ELISA试剂盒、大鼠ELISA试剂盒、兔ELISA试剂盒、鸡ELISA试剂盒
- 主营：人ELISA试剂盒、大鼠ELISA试剂盒、生化法检测试剂盒、小鼠头蛋白、分子学检测试剂盒、植物ELISA试剂盒、鱼ELISA试剂盒
- 主营：头蛋白试剂盒、试剂盒、ELISA试剂盒、ELISA检测试剂盒
- 主营：Elisa试剂盒
- 主营：头蛋白试剂盒、elisa试剂盒、试剂盒、抗体
- 主营：大鼠白介素、表皮生长因子、ELISA试剂盒、小鼠脂联素、重组蛋白、细胞因子
- 主营：氯霉素、试剂盒、胆固醇、糖蛋白、脂蛋白、钙蛋白、调蛋白、粘液素、磷酸酶、人嗅素、饥饿素、磷脂酶、抑制因、凝血酶、连接酶、凝集素、分泌酶、白介素、胰岛素、阻滞剂、结合盒、防御素、纤溶酶、干扰素、板层素
- 主营：试剂盒、同源物、粘液素、脂蛋白、合蛋白、蛋白酶、凝蛋白、抑制素、亲环素、保护素、信号素、胞色素、脂肪酶、末端肽、磷脂酶、变位酶、抑制剂、脱水酶、抑制物、激活肽、连接酶、样激酶、解氨酶、恒温箱、利钠肽
- 主营：瘤细胞、细胞株、肉毒碱、脂蛋白、蛋白酶、切蛋白、样蛋白、信号素、测试盒、癌细胞、羧化酶、子宫肌、质激素、羧肽酶、坏血酸、合成酶、视网膜、同源物、骨钙素、羧激酶、白介素、加压素、纤溶酶、骨桥素、干扰素
- 主营：besc20035、细胞系、人皮肤、小鼠脾、试剂盒、兔结肠、兔膀胱、猪子宫、人胶质、猪骨髓、聚合酶、兔食管、兔胰腺、猪心肌、猪淋巴、bes2016dp、兔气管、兔子宫、besc20114、besc20110、核苷酸、besc20301、兔尿道、兔真皮、兔气道
- 主营：小鼠叉头框蛋白P3、ELISA试剂盒、抗体
- 主营：elisa试剂盒、人elisa试剂盒、大鼠elisa试剂盒、小鼠elisa试剂盒、植物elisa试剂盒、鱼elisa试剂盒、猪elisa试剂盒、牛elisa试剂盒、鸡elisa试剂盒、兔elisa试剂盒、犬elisa试剂盒
- 主营：ELISA试剂盒、PCR试剂盒、生化试剂盒、科研试剂