Overview
The mouse mannose receptor (MR), also known as CD206, is a transmembrane protein belonging to the C-type lectin family. It is primarily expressed on macrophages and dendritic cells, playing a critical role in innate immunity by recognizing carbohydrate patterns on pathogens. The receptor facilitates phagocytosis and antigen presentation, linking innate and adaptive immune responses. MR is composed of multiple domains, including an N-terminal cysteine-rich region, a fibronectin type II domain, and carbohydrate-recognition domains (CRDs). Its ability to bind mannose, fucose, and N-acetylglucosamine makes it a versatile tool for studying host-pathogen interactions and immune modulation.
Physical and Chemical Properties
The mouse MR is a glycoprotein with a molecular weight of approximately 175-180 kDa when glycosylated. It exists as a monomer or dimer under native conditions and is stable in neutral pH buffers like PBS. The receptor's binding affinity is calcium-dependent, with optimal activity at physiological pH (7.2-7.4). In research settings, MR is commonly supplied as a lyophilized powder or in solution. Reconstitution typically requires sterile PBS or similar buffers. Long-term storage at -20°C is recommended for lyophilized forms, while short-term use of solutions can be maintained at 4°C with protease inhibitors.
Main Applications
Mouse MR is widely used in immunology research to study macrophage function, dendritic cell biology, and pathogen recognition. It serves as a marker for M2 macrophages in polarization studies and is exploited in vaccine development to enhance antigen uptake by antigen-presenting cells. In infectious disease research, MR is a key target for understanding interactions with pathogens like Mycobacterium tuberculosis and Candida albicans. Additionally, its role in tumor-associated macrophages makes it relevant for cancer immunotherapy studies. Recombinant MR fragments are often employed in binding assays and structural biology projects.
Safety and Storage
While mouse MR is not classified as highly hazardous, standard laboratory precautions should be followed. Use gloves and eye protection when handling, and avoid generating aerosols. Lyophilized protein is stable for years at -20°C but should be aliquoted after reconstitution to avoid repeated freeze-thaw cycles. For functional assays, ensure proper buffer conditions (e.g., 2-5 mM CaCl₂ for carbohydrate binding). Contamination with endotoxins can affect experimental outcomes, so verify supplier specifications (<1 EU/μg is typical for cell culture applications).
B2B Procurement Guide
When sourcing mouse MR, prioritize suppliers with documented quality controls (e.g., SDS-PAGE purity certificates, endotoxin testing). Research-grade proteins typically cost $200-$500 per mg, while labeled or conjugated variants command higher prices. Consider application-specific requirements: full-length receptors for cell studies versus CRD fragments for binding assays. Lead times vary; recombinant proteins may require 4-8 weeks for custom production. For high-throughput screening, some vendors offer plate-based assay kits. Always confirm batch-to-batch consistency and request technical support documents, especially for first-time purchases.
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