Overview
Mouse β-Galactosidase is an enzyme derived from murine sources, primarily used in laboratory settings for hydrolyzing β-galactoside bonds in substrates like X-Gal and ONPG. It plays a critical role in molecular biology, particularly in reporter gene systems (e.g., lacZ gene expression). The enzyme is available in recombinant forms for consistent performance. Its specificity and stability make it a preferred choice for assays requiring precise quantification of gene activity. Commercial preparations often include stabilizers to maintain activity during storage and shipment.
Physical and Chemical Properties
Mouse β-Galactosidase typically exists as a tetramer with a monomeric molecular weight of ~116 kDa. It exhibits optimal activity at pH 7.0–7.5 and temperatures around 37°C, though activity declines sharply above 50°C. The enzyme requires magnesium ions as cofactors for full functionality. In solution, it appears colorless or slightly yellow. Lyophilized forms are stable for years when stored properly, while liquid formulations may include glycerol or other cryoprotectants to prevent aggregation.
Main Applications
The enzyme is indispensable in lacZ-based reporter systems, where it cleaves chromogenic substrates (e.g., X-Gal) to produce visible signals for gene expression analysis. It’s also used in ELISA-like assays and histochemistry to localize enzyme activity in tissues. Beyond research, it finds niche applications in lactose-free product development and diagnostic kits. Its ability to hydrolyze synthetic substrates enables high-throughput screening in drug discovery pipelines.
Safety and Storage
While not highly toxic, direct exposure to β-galactosidase powder or concentrated solutions may cause irritation. Use gloves and goggles, and work in a fume hood when handling large quantities. Spills should be neutralized with water and cleaned promptly. For storage, lyophilized enzyme should be kept at -20°C in a desiccated environment. Liquid formulations often require refrigeration (4°C) but avoid freezing unless specified by the manufacturer. Activity loss can occur if repeatedly thawed or exposed to light.
B2B Procurement Guide
When sourcing Mouse β-Galactosidase, prioritize suppliers providing certificates of analysis (COA) detailing purity, specific activity (e.g., ≥300 units/mg), and endotoxin levels. Bulk buyers should negotiate batch-to-batch consistency clauses. Consider recombinant variants for higher purity and lower lot variability. For specialized applications (e.g., in vivo studies), request endotoxin-free or low-pyrogen versions. Price varies by quantity and purity; bulk orders (10+ mg) may reduce costs by 20–30%.
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