Overview
Mouse Endostatin is a 20 kDa C-terminal fragment of collagen XVIII, first identified for its ability to inhibit angiogenesis. It functions by binding to integrins and other receptors on endothelial cells, disrupting signaling pathways critical for blood vessel formation. The murine variant is widely used in preclinical studies due to its homology to human endostatin and relevance in mouse models. As a research tool, it is typically produced via recombinant DNA technology in E. coli or mammalian expression systems. Its mechanism involves suppression of VEGF signaling and induction of endothelial cell apoptosis, making it a key molecule for studying tumor microenvironments and anti-cancer strategies.
Physical and Chemical Properties
Mouse Endostatin is a globular protein with high stability in pH 6-8 buffers. It maintains activity after lyophilization and can be reconstituted in sterile PBS or culture media. Circular dichroism studies show a structure rich in β-sheets, contributing to its thermal stability up to 60°C. The protein is typically quantified by UV absorbance (ε280 ≈ 1.4 mL·mg⁻¹·cm⁻¹) and analyzed via SDS-PAGE or HPLC. Impurities like host cell proteins or endotoxins are critical quality parameters, as they may affect experimental outcomes in sensitive cell-based assays.
Main Applications
In oncology research, Mouse Endostatin is utilized to study angiogenesis inhibition in xenograft models and metastasis assays. It serves as a positive control in drug screening platforms evaluating novel anti-angiogenic compounds. Researchers also employ it to create ischemic conditions in tissue engineering studies. Beyond cancer, applications include ophthalmology (retinopathy models) and cardiovascular research (atherosclerosis). Some B2B buyers procure it for biosimilar development, as human endostatin analogs have entered clinical trials as adjunctive cancer therapies.
Safety and Storage
While not classified as hazardous, proper aseptic handling is recommended to maintain sterility for cell culture applications. Lyophilized material should be stored at -20°C or lower with desiccant to prevent moisture absorption, which can lead to aggregation. Reconstituted solutions are stable for 1-2 weeks at 4°C. Shipping typically requires cold chain logistics (dry ice for international transport). Vendors should provide certificates of analysis detailing endotoxin levels, which must be <1 EU/μg for in vivo studies to prevent inflammatory responses in animal models.
B2B Procurement Guide
When sourcing Mouse Endostatin, prioritize suppliers with ISO 13485 or GMP certification for consistency. Key specifications to request include: 1) Purity by HPLC (>95%), 2) Biological activity (typically ED50 <100 ng/mL in HUVEC assays), and 3) Sterility testing results. Bulk orders (100mg+) may qualify for 15-30% discounts. Consider ordering small test quantities to validate performance in your specific assay system before large-scale purchase. Some vendors offer custom formulations (e.g., fluorescently labeled variants) for specialized imaging applications at 20-50% premium pricing.
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