Overview
Mouse E-Cadherin (Cadherin-1) is a transmembrane glycoprotein essential for Ca²⁺-dependent cell-cell adhesion in epithelial tissues. As a classical cadherin, it plays pivotal roles in tissue morphogenesis, maintaining epithelial integrity, and cellular signaling pathways. The protein consists of five extracellular cadherin repeats, a transmembrane domain, and a highly conserved cytoplasmic tail that interacts with catenins. In research contexts, mouse E-Cadherin is widely used to study epithelial-mesenchymal transition (EMT), a critical process in embryonic development and cancer metastasis. Commercially available preparations include recombinant proteins (full-length or extracellular domain), antibodies, and ELISA kits for quantitative analysis. Researchers should note that mouse E-Cadherin shares approximately 90% amino acid sequence identity with human CDH1.
Physical and Chemical Properties
Mouse E-Cadherin exhibits calcium-dependent homophilic binding, requiring 1-2 mM Ca²⁺ for proper folding and function. The extracellular domain mediates dimerization through strand-swapping mechanisms, while the cytoplasmic domain binds β-catenin and p120-catenin to link with the actin cytoskeleton. Glycosylation at multiple sites contributes to its apparent molecular weight of ~120 kDa on SDS-PAGE. The protein is sensitive to proteolytic degradation, especially in calcium-free conditions where it becomes vulnerable to trypsin cleavage. For laboratory use, formulations typically contain stabilizing agents like BSA or glycerol. Common buffers for reconstitution include Tris-HCl (pH 7.4-8.0) or PBS with 1-2 mM CaCl₂. Activity is typically measured by cell aggregation assays or surface plasmon resonance (SPR) binding studies.
Main Applications
In cancer research, mouse E-Cadherin serves as a key epithelial marker whose downregulation correlates with increased metastatic potential. Researchers employ it to investigate EMT in tumor models, often using knockout mice or 3D organoid cultures. The protein is also crucial for studying cell polarity and junction formation in developmental biology. Pharmaceutical applications include screening for cadherin-targeting drugs that modulate cell adhesion. In biotechnology, E-Cadherin-coated surfaces are used for selective epithelial cell culture. Diagnostic applications involve detecting soluble E-Cadherin fragments (sE-Cad) as potential biomarkers for epithelial-derived cancers. Recent advances include its use in bioengineered tissues requiring precise cell positioning.
Safety and Storage
Mouse E-Cadherin preparations are generally classified as Risk Group 1 biologicals, requiring standard BSL-1 handling. However, endotoxin levels should be verified (<1 EU/μg) for cell culture applications. Lyophilized proteins are stable for 12-24 months at -80°C, while liquid formulations (often containing 0.1% BSA) maintain activity for 3-6 months at -20°C. Repeated freeze-thaw cycles should be avoided; aliquotting is recommended. For functional studies, verify calcium concentration in assay buffers (1-2 mM optimal). Contamination risks include bacterial proteases that may degrade the protein. Suppliers typically provide certificates of analysis detailing purity (typically >95% by SDS-PAGE), endotoxin levels, and functional activity data.
B2B Procurement Guide
When sourcing mouse E-Cadherin, prioritize suppliers with ISO 13485 certification for consistent quality. Key specifications include: 1) Origin (recombinant vs. tissue-derived), 2) Purity level (≥90% for most applications), 3) Endotoxin content (<0.1 EU/μg for sensitive assays), and 4) Functional validation data (e.g., cell adhesion inhibition assays). Bulk purchasers (≥10 mg) should negotiate batch-to-batch consistency guarantees. Consider custom services like fluorophore labeling or biotinylation for specialized applications. Lead times for recombinant proteins typically range 4-8 weeks. For GMP-grade material (required for therapeutic development), expect 2-3x price premiums. Reliable suppliers include R&D Systems, Sino Biological, and Abcam, with prices varying by purity and formulation.
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