Mouse Collagen Type XV

Updated: 2026-08-25

Overview

Mouse Type XV Collagen is a member of the multiplexin collagen family, characterized by its interrupted triple-helical structure and non-fibrillar nature. It plays critical roles in maintaining tissue integrity, particularly in basement membranes where it interacts with other extracellular matrix components. Unlike fibrillar collagens, Type XV exhibits unique N-terminal domains that facilitate cell-matrix signaling. This collagen isoform is extensively studied in murine models due to its homology with human COL15A1 and its implications in developmental biology. Researchers value its tissue-specific expression patterns in organs like kidneys, blood vessels, and skeletal muscles, making it a valuable tool for understanding extracellular matrix remodeling.

Physical and Chemical Properties

Type XV Collagen forms a complex network of disulfide-bonded trimers with distinctive interruptions in its Gly-X-Y repeat sequence. The protein demonstrates thermolability, undergoing denaturation at relatively low temperatures compared to structural collagens. Its solubility profile requires acidic buffers (pH 3-4) for reconstitution, with optimal stability achieved at 4°C. Characterization typically involves SDS-PAGE (showing ~200-250 kDa bands under reducing conditions) and mass spectrometry. The molecule's susceptibility to collagenase digestion distinguishes it from other ECM proteins. Fourier-transform infrared spectroscopy (FTIR) often confirms its secondary structure, showing characteristic amide I and II peaks at ~1650 cm⁻¹ and 1550 cm⁻¹ respectively.

Main Applications

In biomedical research, Mouse Type XV Collagen serves as a substrate for studying tumor microenvironments due to its role in modulating angiogenesis. Its anti-angiogenic properties make it particularly relevant for cancer metastasis investigations. Laboratories frequently incorporate it into 3D cell culture systems to mimic native tissue conditions. The protein also finds application in cardiovascular research, where its presence in vascular basement membranes contributes to studies on endothelial cell behavior. Recent advancements in regenerative medicine utilize recombinant mouse Type XV Collagen in scaffold formulations for organ-specific tissue engineering, especially in renal and musculoskeletal systems.

Safety and Storage

While not classified as hazardous, proper handling requires gloves and eye protection to prevent potential allergic sensitization. Lyophilized preparations should be reconstituted under sterile conditions to maintain endotoxin-free status. Long-term storage at -20°C in desiccated environments preserves stability, with aliquoting recommended to minimize freeze-thaw degradation. Quality control measures should include testing for microbial contamination and confirming absence of protease activity. Material Safety Data Sheets (MSDS) typically categorize this collagen as Risk Group 1, but institution-specific biosafety protocols should always be consulted before use in biological systems.

B2B Procurement Guide

When sourcing Mouse Type XV Collagen, prioritize suppliers providing comprehensive characterization data including HPLC purity profiles, Western blot verification, and endotoxin certification. Bulk purchasers should request batch-to-batch consistency reports and consider stability studies if long-term projects are planned. For specialized applications like in vivo studies, verify the absence of xenoantigens through supplier documentation. Many manufacturers offer custom services including fluorescent labeling or peptide fragment production. Lead times for research-grade material typically range 4-8 weeks, with expedited processing available at premium costs.