Overview
Mouse Apolipoprotein C3 (ApoC3) is a 79-amino acid protein predominantly synthesized in the liver and intestine. As a component of very-low-density lipoproteins (VLDL) and chylomicrons, it plays a crucial regulatory role in triglyceride metabolism. In research settings, recombinant mouse ApoC3 is widely used to study lipid disorders, atherosclerosis, and metabolic syndromes. This protein is particularly valuable for creating genetically modified mouse models to investigate human diseases. Its sequence homology with human ApoC3 (approximately 70%) makes it relevant for translational studies. Researchers frequently employ it in ELISA assays, Western blotting, and functional studies of lipoprotein metabolism.
Physical and Chemical Properties
Mouse ApoC3 has a molecular weight of approximately 8.8 kDa and exhibits an isoelectric point around pH 4.5-5.0. The protein lacks disulfide bonds but contains several phosphorylation sites that may modulate its function. In solution, it demonstrates stability across a pH range of 6-8 when stored at recommended temperatures. The protein's amphipathic helix structure enables lipid binding, particularly to triglyceride-rich lipoproteins. Its solubility in aqueous buffers makes it suitable for in vitro experiments, though aggregation may occur at high concentrations. Analytical techniques like mass spectrometry and circular dichroism are commonly used to characterize its structural properties.
Main Applications
In biomedical research, mouse ApoC3 serves three primary purposes: as a metabolic regulator study tool, a disease biomarker, and a therapeutic target candidate. Researchers utilize it to investigate hypertriglyceridemia mechanisms, where ApoC3 overexpression leads to impaired lipoprotein lipase activity and reduced triglyceride clearance. The protein is essential for creating atherosclerosis models, as elevated ApoC3 levels correlate with increased cardiovascular risk. Pharmaceutical studies focus on developing ApoC3 inhibitors, making the mouse homolog valuable for preclinical testing. Additionally, it's used in nutrigenomics research to examine dietary effects on lipid metabolism.
Safety and Storage
Mouse ApoC3 is classified as a Biosafety Level 1 material, requiring standard laboratory handling procedures. While non-toxic, proper personal protective equipment (gloves, lab coat) should be worn to prevent contamination. The lyophilized form is stable for years at -20°C when kept desiccated. Reconstituted solutions should be aliquoted to avoid repeated freeze-thaw cycles, which can degrade protein integrity. For short-term use, solutions may be stored at 4°C for up to one week. Always verify protein concentration and purity after prolonged storage using spectrophotometry or electrophoresis methods.
B2B Procurement Guide
When sourcing mouse ApoC3, prioritize suppliers specializing in research-grade recombinant proteins. Key specifications include ≥95% purity (verified by SDS-PAGE and HPLC), low endotoxin levels (<1 EU/μg), and proper bioactivity validation. Consider whether you need native sequence or tagged versions (e.g., His-tag) for your experimental workflow. For large-scale studies, inquire about bulk pricing and custom modification options. Reputable suppliers should provide detailed characterization data, including mass spectrometry analysis and functional assay results. Lead times typically range from 2-6 weeks for specialty items. Always request certificates of analysis and material safety data sheets with your order.
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