Overview
Micrococcus lysodeikticus, now more commonly referred to as Micrococcus luteus, is a non-pathogenic, Gram-positive bacterium that forms yellow to orange colonies. It is widely known for its use in lysozyme assays due to its high susceptibility to this enzyme. Originally isolated from environmental sources, this bacterium has become a standard tool in microbiological and biochemical laboratories. Its non-pathogenic nature and predictable behavior make it ideal for educational and research purposes.
Physical and Chemical Properties
Micrococcus lysodeikticus is characterized by its spherical cells, typically arranged in tetrads or irregular clusters. The bacterium thrives in aerobic conditions and exhibits optimal growth at temperatures around 25-30°C. Its cell wall composition, rich in peptidoglycan, makes it highly susceptible to lysozyme, which hydrolyzes the glycosidic bonds in the peptidoglycan layer. This property is exploited in lysozyme activity assays and related research applications.
Main Applications
The primary application of Micrococcus lysodeikticus is in lysozyme assays, where it serves as a substrate to measure enzyme activity. It is also used in educational settings to demonstrate basic microbiological techniques and Gram staining. In industrial microbiology, this bacterium is employed in quality control processes and as a model organism for studying bacterial cell wall structure and function. Its non-pathogenic nature makes it a safe choice for various laboratory applications.
Safety and Storage
While Micrococcus lysodeikticus is non-pathogenic, standard microbiological practices should be followed when handling it. This includes wearing appropriate personal protective equipment and working in a controlled environment. For long-term storage, the bacterium is typically lyophilized and kept at -20°C or below. Proper labeling and documentation are essential to ensure strain authenticity and traceability.
B2B Procurement Guide
When procuring Micrococcus lysodeikticus, it is crucial to verify the supplier's reputation and the strain's authenticity. Request certificates of analysis and ensure that the strain meets your specific requirements. Consider purchasing from reputable culture collections or specialized suppliers to guarantee quality. Bulk orders may offer cost advantages, but ensure that storage conditions can accommodate the quantity purchased.
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