Overview
Methyl Red is an azo dye widely recognized as a pH indicator in analytical chemistry and microbiological applications. First synthesized in the early 20th century, it remains a standard reagent in laboratories worldwide. The compound's distinct color change between pH 4.4 (red) and 6.2 (yellow) makes it particularly valuable for titration experiments and microbial culture media. The term 'analytical purity' (分析纯) refers to reagents meeting Chinese GB/T standards for laboratory use, typically with purity ≥99%. Methyl Red of analytical grade undergoes rigorous testing for impurities like heavy metals and insolubles, ensuring consistent performance in sensitive applications.
Physical and Chemical Properties
As an azo compound, Methyl Red features a diazo (-N=N-) group connecting aromatic rings, which accounts for its vivid coloration and pH sensitivity. The crystalline powder shows moderate stability under normal conditions but may degrade upon prolonged exposure to strong light or oxidizing agents. Its limited water solubility necessitates ethanol or acetic acid as preferred solvents for preparing indicator solutions. Thermal analysis reveals decomposition before boiling, with the melting point serving as a key quality control parameter. The molecular weight (269.30 g/mol) is critical for preparing molar solutions. When dissolved, the compound exhibits maximum absorbance at 410-430 nm, a property utilized in spectrophotometric methods.
Main Applications
In microbiology, Methyl Red forms part of the MR-VP test for identifying enteric bacteria based on their glucose fermentation patterns. Escherichia coli typically produces a positive red result, while Enterobacter species yield negative (yellow) readings. This application remains vital in clinical and food safety testing. The dye also serves in educational chemistry demonstrations, industrial process monitoring, and as a component in multi-range pH test papers. Recent research explores its potential in photodynamic therapy and as a staining agent for microscopic analysis, though these applications require specialized purity grades beyond standard analytical reagents.
Safety and Storage
As a moderately hazardous substance, Methyl Red requires proper handling with PPE including gloves and eye protection. The powder can generate irritant dust, necessitating use in fume hoods during weighing. Spills should be contained with absorbent materials and cleaned promptly to prevent environmental contamination. Long-term storage stability is achieved in amber glass bottles at room temperature (15-25°C) with low humidity. Suppliers typically provide a shelf life of 2-3 years from production date. Degradation signs include color fading or solution precipitation. For bulk quantities, climate-controlled warehouses with fire prevention measures are recommended due to the compound's combustible nature.
B2B Procurement Guide
Industrial buyers should prioritize suppliers with ISO 9001 certification and batch-specific Certificates of Analysis (CoA). Key specifications include dye content (≥95% for analytical grade), pH transition range verification, and heavy metal limits (typically <10ppm). Packaging options range from 25g vials to 25kg fiber drums, with larger volumes offering better per-unit costs. Due to fluctuating raw material costs, request updated price lists and consider long-term contracts for stable supply. Verify shipping complies with chemical transport regulations (UN proper shipping name: 'Environmentally hazardous substance, solid, n.o.s.'). For international procurement, confirm the supplier's experience with export documentation including MSDS, commercial invoices, and hazardous material declarations.
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