Leucine Alpha-2-glycoprotein Antibody

Updated: 2026-07-22

Overview

Leucine Alpha-2-Glycoprotein (LAG) Antibody is a monoclonal or polyclonal antibody designed to target the LAG protein, which plays a role in immune regulation and is implicated in conditions like cancer and autoimmune diseases. It is widely utilized in laboratory research and clinical diagnostics. The antibody is produced through immunization of host animals with purified LAG protein, followed by purification processes to ensure high specificity and minimal cross-reactivity. Its applications span Western blotting, ELISA, and immunohistochemistry.

Physical and Chemical Properties

LAG Antibody typically presents as a clear to slightly cloudy liquid, soluble in standard aqueous buffers such as PBS or Tris-HCl. It is stable under recommended storage conditions but may degrade if exposed to repeated freeze-thaw cycles or extreme temperatures. The antibody's specificity is confirmed through rigorous testing against related proteins to ensure minimal off-target binding. Its affinity is quantified using methods like surface plasmon resonance (SPR) or ELISA, with typical dissociation constants (Kd) in the nanomolar range.

Main Applications

LAG Antibody is primarily used in research to study the role of LAG in immune responses and disease mechanisms. It is a critical tool for detecting LAG expression in tissues or serum samples, aiding in the diagnosis of conditions like rheumatoid arthritis or certain cancers. In clinical settings, the antibody may be employed in diagnostic kits to monitor disease progression or treatment efficacy. Its high specificity makes it valuable for validating LAG as a biomarker in translational research.

Safety and Storage

Proper handling of LAG Antibody requires adherence to biosafety guidelines, including the use of gloves and lab coats. Avoid skin contact or inhalation, as some formulations may contain preservatives like sodium azide, which is toxic. For long-term stability, store the antibody at -20°C in aliquots to prevent repeated freeze-thaw cycles. Short-term storage at 4°C is acceptable for frequently used samples, but prolonged exposure to higher temperatures should be avoided.

B2B Procurement Guide

When procuring LAG Antibody, prioritize suppliers with certifications like ISO 13485 or GMP compliance. Request batch-specific certificates of analysis (CoA) to verify purity, specificity, and endotoxin levels. Consider the antibody's format (e.g., lyophilized vs. liquid) and compatibility with your intended applications. Bulk purchases may offer cost savings, but ensure proper storage capacity to maintain product integrity.