Overview
The leptin receptor (LEPR) is a class I cytokine receptor primarily expressed in the hypothalamus, where it mediates the effects of leptin—a hormone critical for regulating appetite, energy expenditure, and body weight. Discovered in 1995, LEPR exists in multiple isoforms (e.g., LEPR-B, the longest and functionally active form) generated by alternative splicing. Mutations in LEPR are linked to severe obesity and metabolic syndromes in humans, underscoring its physiological importance. LEPR functions as a transmembrane protein, activating intracellular signaling pathways such as JAK-STAT upon leptin binding. Its role extends beyond metabolism, influencing immune responses, bone formation, and reproduction. Research tools like recombinant LEPR and antibodies are widely used in studying these pathways and developing therapeutics for obesity-related diseases.
Physical and Chemical Properties
LEPR is a glycoprotein with a molecular weight ranging from 130 to 150 kDa, depending on the isoform and glycosylation state. It consists of an extracellular leptin-binding domain, a transmembrane region, and an intracellular domain responsible for signal transduction. The receptor’s solubility depends on its formulation; commercial preparations are often provided in buffered solutions or lyophilized powders for reconstitution. Key biochemical properties include high affinity for leptin (Kd ~1-10 nM) and activation of downstream kinases like JAK2. Stability varies: liquid formulations typically require storage at -20°C or below, while lyophilized versions may tolerate short-term refrigeration. Purity is assessed via SDS-PAGE (>90% preferred), and functionality is confirmed through binding assays (e.g., ELISA or surface plasmon resonance).
Main Applications
LEPR is pivotal in obesity and metabolic research, particularly in elucidating leptin resistance—a hallmark of type 2 diabetes and obesity. Researchers use recombinant LEPR or cell lines expressing the receptor to screen for leptin mimetics or antagonists, aiming to develop drugs that modulate this pathway. Beyond therapeutics, LEPR studies contribute to understanding endocrine cross-talk, such as its interaction with insulin signaling. In agriculture, LEPR variants are explored for livestock breeding to enhance lean meat production. Diagnostic applications include detecting LEPR mutations in genetic testing for early-onset obesity.
Safety and Storage
While LEPR is non-toxic, standard biosafety protocols (e.g., gloves, lab coats) are recommended to prevent contamination. Avoid repeated freeze-thaw cycles, which can degrade protein activity. For long-term storage, aliquot solutions and store at -80°C; lyophilized powder is stable at -20°C if kept desiccated. Handling requires sterile techniques for cell culture or in vivo applications. Vendor-provided Material Safety Data Sheets (MSDS) should be reviewed for specific hazards, though risks are typically minimal (e.g., mild irritant in high concentrations). Dispose of waste following institutional guidelines for biological materials.
B2B Procurement Guide
When procuring LEPR for research or industrial use, prioritize vendors with ISO certification and batch-specific quality control data. Key criteria include isoform specificity (e.g., LEPR-B for full signaling activity), purity (>90% by SDS-PAGE), and functional validation (e.g., binding affinity data). Pricing varies by quantity and purity; bulk purchases (e.g., >10 mg) may reduce costs by 20-30%. Consider custom services like conjugation (biotin, fluorescent tags) for specialized assays. Lead times range from 2-6 weeks for off-the-shelf products, longer for custom orders. Negotiate supply agreements for recurring needs to ensure consistency.
Related Manufacturers
- 主营：人瘦素受体检测试剂盒、ELISA试剂盒、PCR试剂盒、科研试剂
- 主营：ELISA试剂盒、PCR试剂盒、生化试剂盒、瘦素受体ELISA试剂盒、蛋白、抗体、细胞、检测试剂盒
- 主营：试剂盒、elisa试剂盒、细胞、pcr试剂盒
- 主营：科研试剂盒、酶免试剂盒、elisa试剂盒、小鼠可溶性瘦素受体、细胞、生物试剂、进口ELISA试剂盒、PCR试剂盒、生化测试盒
- 主营：ELISA试剂盒、PCR试剂盒、生化试剂盒、瘦素受体ELISA试剂盒、蛋白、抗体、细胞
- 主营：检测方、钠尿肽、试剂盒、猪瘦素受体、同源物、ep300检测、结合蛋白、大鼠c-肽(、微球蛋白、抗胰蛋白酶、淋巴瘤因子、蛋白磷酸酶、死亡激动剂、核心域蛋白1、大鼠e选择素、大鼠janus激酶、激活蛋白激酶、大鼠ⅱ型胶原、大鼠ⅳ型胶原、大鼠klotho蛋白、细胞激活因子、大鼠ⅲ型胶原、大鼠nadh脱氢酶1、大鼠ⅵ型胶原α1、elisa 检测试剂盒
- 主营：ELISA试剂盒、PCR试剂盒、生化试剂盒、脂蛋白、关基因、亲环素、钾通道、列抗体、ntp溶液、蛋白酶、脑蛋白、病毒抗、蛋白质、239抗体、dtt溶液、科研试剂
- 主营：科研试剂盒、酶免试剂盒、elisa试剂盒、猪瘦素受体、生化试剂盒、细胞、生物试剂、进口ELISA试剂盒、PCR试剂盒、生化测试盒
- 主营：酶cγ链、α2巨球、素d合成、人瘦素受体elisa试剂盒、纤连蛋白、抗组蛋白、肌醇抗体、蛋白样环、敏感蛋白、牛骨钙素、蛋白重链、金属肽酶、乳球蛋白、钩端螺旋、蛋白激酶、高血糖素、钙调蛋白、小鼠α1抗、ELISA试剂盒、pcr检测试剂、生化检测试剂
- 主营：人瘦素受体elisa试剂盒、试剂盒、人25羟基维生素、人低氧诱导因子
- 主营：ELISA试剂盒、PCR试剂盒、生化试剂盒、科研试剂
- 主营：大鼠瘦素受体、ELISA试剂盒、抗体
- 主营：白细胞介素、生物试剂、甲状腺素、人瘦素受体试剂盒、活性氧簇、促甲状腺激素、孕酮、促黄体生成素、心肌肌钙蛋白、检测试剂盒、植物试剂盒、试剂盒、实验室试剂、Elisa、定量分析、定性分析、酶联免疫试剂盒、科研试剂盒、免费代测
- 主营：Elisa试剂盒、PCR试剂盒、PCR检测、可溶性瘦素受体elisa、PCR检测试剂盒、核酸检测试剂盒、荧光定量检测试剂盒、荧光定量试剂盒、比色法检测试剂盒、生化试剂盒、比色法试剂盒、酶活性比色法检测试剂盒、酶活性检测试剂盒、微量法检测试剂盒、分光光度检测试剂盒、酶活分光光度试剂盒、ELISA试剂盒、ELISA检测、酶联免疫试剂盒、酶联免疫检测试剂盒、酶联免疫、细胞、抗体
- 主营：人可溶性瘦素受体、检测试剂盒、elisa试剂盒