Labeled Phosphoramidite

Updated: 2026-09-11

Overview

Labeled Phosphoramidite is a specialized chemical reagent crucial for the synthesis of modified oligonucleotides. It is widely used to introduce labels such as fluorescent dyes, biotin, or other markers into DNA or RNA strands during solid-phase synthesis. This compound plays a vital role in molecular biology research, diagnostics, and therapeutic development. Its reactive phosphoramidite group allows efficient coupling to growing oligonucleotide chains, while the label moiety enables detection, purification, or functional studies. The choice of label depends on the intended application, with common options including fluorescein, cyanine dyes, and digoxigenin.

Physical and Chemical Properties

Labeled Phosphoramidites are typically supplied as moisture-sensitive powders or crystalline solids. They are soluble in anhydrous organic solvents like acetonitrile, which is commonly used in automated DNA synthesizers. The compounds are highly reactive and must be handled under inert conditions to prevent degradation. The stability of these reagents varies depending on the specific label and protecting groups employed. Most commercial products are stabilized with drying agents and shipped under argon or nitrogen atmosphere. Proper storage at -20°C or lower is recommended for long-term preservation of activity.

Main Applications

The primary use of Labeled Phosphoramidite is in the synthesis of labeled oligonucleotides for research and diagnostic applications. These labeled probes are essential tools in techniques such as fluorescence in situ hybridization (FISH), quantitative PCR, microarray analysis, and next-generation sequencing. In pharmaceutical development, labeled oligonucleotides are used for target validation, drug screening, and pharmacokinetic studies. The biotechnology industry employs these compounds to create diagnostic probes and therapeutic oligonucleotides with tracking capabilities. Recent advances have expanded their use in CRISPR gene editing and RNA interference technologies.

Safety and Storage

Labeled Phosphoramidites require careful handling due to their moisture sensitivity and potential toxicity. Always work in a fume hood with appropriate personal protective equipment, including gloves and safety glasses. Exposure to air should be minimized, and reagents should be kept under inert gas when not in use. Long-term storage should be in airtight containers with desiccant, preferably at -20°C or lower. Some labeled derivatives may be light-sensitive and require amber vials. Proper disposal methods should be followed according to local regulations for chemical waste, particularly for fluorescent-labeled compounds which may have environmental concerns.

B2B Procurement Guide

When procuring Labeled Phosphoramidites, consider the specific label required for your application, the purity grade (typically 95-99%), and the scale of synthesis needed. Bulk purchases for industrial applications may offer cost savings but require verification of batch-to-batch consistency. Key suppliers include specialized chemical manufacturers and oligonucleotide synthesis service providers. Request certificates of analysis for each batch, and consider small test quantities before large purchases. Lead times can vary significantly depending on the complexity of the label, so plan procurement accordingly for research or production timelines.