Overview
King's B Medium is a specialized microbiological growth medium developed in 1954 by Elizabeth O. King for the isolation and identification of Pseudomonas species. The medium contains peptone, magnesium chloride, potassium sulfate, and glycerol, which collectively promote the production of fluorescent pigments by Pseudomonas bacteria. This medium is particularly valued for its ability to enhance the fluorescence of pyoverdine and pyocyanin pigments, making it invaluable for Pseudomonas detection and differentiation. It has become a standard tool in environmental microbiology, plant pathology studies, and industrial quality control processes where Pseudomonas detection is critical.
Physical and Chemical Properties
As a complex medium, King's B consists of multiple organic and inorganic components. The base formulation includes 20g/L proteose peptone, 1.5g/L dipotassium phosphate, 1.5g/L magnesium sulfate, and 10mL/L glycerol. When prepared, the medium typically has a pH of 7.2±0.2 after sterilization. The glycerol component is particularly important as it serves as a carbon source that enhances pigment production. The magnesium sulfate promotes fluorescence development in Pseudomonas species. The medium solidifies to a firm gel when agar (typically 15g/L) is added, with a smooth, slightly opaque appearance when properly prepared.
Main Applications
The primary application of King's B Medium is in the isolation and identification of fluorescent Pseudomonas species from various samples. It is extensively used in plant pathology laboratories to detect plant-associated Pseudomonas pathogens. The medium's ability to promote pigment fluorescence allows for easy visual identification under UV light (typically at 366nm). In industrial settings, King's B Medium serves as a quality control tool for detecting Pseudomonas contamination in pharmaceutical and cosmetic products. Environmental microbiologists use it to study Pseudomonas populations in soil and water samples. Recent applications include biofilm research and studies on antibiotic resistance mechanisms in Pseudomonas species.
Safety and Storage
The dry powder form of King's B Medium is stable at room temperature when kept in sealed containers with desiccants. Prepared media should be stored at 2-8°C and used within 1-2 weeks to prevent dehydration or contamination. Always inspect prepared plates for contamination or drying before use. When working with cultures grown on King's B Medium, standard biosafety level 2 precautions are recommended, particularly with clinical or environmental isolates. The medium itself is non-hazardous, but cultures may contain pathogenic organisms. Autoclave used media at 121°C for at least 15 minutes before disposal.
B2B Procurement Guide
When procuring King's B Medium for laboratory use, consider the intended application scale and required certifications. Research-grade medium typically offers higher purity and performance consistency compared to industrial-grade products. Key procurement factors include batch-to-batch consistency, sterility guarantees, and technical support availability. For large-scale or regulated applications (e.g., pharmaceutical QC), request certificates of analysis and consider vendor qualification. Many suppliers offer both pre-poured plates and bulk powder formulations - the choice depends on laboratory workflow and storage capacity. Lead times can vary significantly, so plan procurement accordingly, especially for time-sensitive research projects.
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