Overview
High-resolution agarose is a specialized polysaccharide extracted from red algae, engineered for superior performance in molecular separation techniques. Unlike standard agarose, it undergoes additional purification to remove sulfates and pyrogens, resulting in minimal electroendosmosis (EEO) and high gel clarity. Its uniform pore structure enables precise nucleic acid separation, making it indispensable for research, clinical diagnostics, and pharmaceutical quality control. First introduced in the 1980s, this grade of agarose revolutionized gel electrophoresis by allowing sharper band resolution, particularly for fragments between 50 bp and 10 kb. Modern variants may include modifiers like SYBR® Safe compatibility for UV visualization.
Physical and Chemical Properties
High-resolution agarose forms thermoreversible hydrogels at 1-4% concentrations, with gelation occurring at 35-42°C and melting at 85-95°C. Its low EEO (≤0.10) minimizes buffer flow during electrophoresis, preventing band distortion. The polymer's helical bundles create pores of 50-200 nm, optimized for DNA sieving. Key metrics include gel strength (≥1200 g/cm²) and sulfate content (≤0.15%), which affect resolution and background staining. The material is stable at room temperature but degrades under prolonged UV exposure or acidic conditions (<pH 4).
Main Applications
In molecular biology, high-resolution agarose is the matrix of choice for Southern/Northern blots, PCR product analysis, and restriction enzyme mapping. Its ability to resolve small size differences (5-10%) makes it critical for genetic testing, including SNP analysis and CRISPR editing verification. Beyond nucleic acids, modified versions separate proteins via native PAGE or immunodiffusion assays. Industrial applications include vaccine purity testing and plasmid DNA purification in biomanufacturing. Recent advances employ it in microfluidic devices for point-of-care diagnostics.
Safety and Storage
While non-hazardous, powdered agarose can cause respiratory irritation if inhaled. Always use fume hoods when weighing, and wear heat-resistant gloves when handling molten gel (60-70°C). Spills form slippery films—clean immediately with water. Store in sealed containers with desiccants to prevent moisture absorption, which reduces gel strength. Shelf life typically exceeds 3 years if kept at <30°C and <60% humidity. Discard discolored batches (yellowing indicates degradation).
B2B Procurement Guide
For bulk purchases (1kg+), request certificates of analysis for EEO, gelling temperature, and nucleic acid recovery rates. Reputable suppliers provide lot-specific data. Consider gamma-irradiated sterile grades for clinical applications. Pricing tiers depend on purity: standard molecular biology grade (≤0.15% sulfate) suits most labs, while ultrapure (<0.10%) is needed for sensitive applications like next-generation sequencing library prep. For cost efficiency, evaluate bulk discounts versus pre-cast gel options.
Related Manufacturers
- 主营：琼脂糖、l-色氨酸、l-精氨酸、实验试剂
- 主营：人流感、m117-500g、脂蛋白、高分辨率琼脂糖、0189-100g、猪睾酮、人胰岛、赖氨酸、柚皮素、人新生、人蛋白、e122-100g、柚皮苷、人戊肝、磷脂酶、蛋白酶、fishelisa、皂苷rg3、酮戊二、人轮状、人胆酸、j636-100g、胰多肽、厚朴酚、噻唑蓝、甜菊苷
- 主营：加帽酶、激活剂、甘氨酸、琼脂糖、igg抗体、稳定液、玻璃板、试剂盒、脂蛋白、超纯水、激动剂、胆固醇、盐酸盐、因质粒、扩容剂、组化笔、驴抗人、预制胶、驴抗兔、生物驴、草甘膦、重组酶、生物素、曲拉通、去除剂、封闭液
- 主营：叔丁酯、银杏叶、灭菌磷、苯基琼脂糖凝胶6FF、檀香醇、磺酰基、庚烯酮、分散蓝、异丁酯、乙霉威、霜霉威、3681-82-1、水中氟、子烯酮、野菊花、西洋参、氟苯基、芫根苷、甲氨基、唑螨酯、洋艾素、denhardts、水中钙、独活素、苯基芴、水中钡
- 主营：化学品、香精香料、试剂、琼脂糖、中间体、有机硅、橡胶树脂、催化剂、纳米材料
- 主营：黄丹粉、柠檬酸、导热油、琼脂糖价格、漂白粉、707乳液、氧化铝、氯化钙、木质素、防腐剂、除锈剂、防锈剂、酒石酸、抗氧剂、松香工业、碳酸氢铵、醋酸铅、碳酸铅
- 主营：β-环状糊精、阿拉伯胶、刺槐豆胶、琼脂糖、瓜尔豆胶、果胶、海藻酸丙二醇酯、海藻酸钠、黄原胶、结冷胶、聚丙烯酸钠、卡波姆、卡拉胶、魔芋粉、塔拉胶、沙蒿籽胶、明胶、温轮胶、大豆分离蛋白、低聚半乳糖、菊粉、水苏糖、罗汉果甜苷、木聚糖、索马甜、氨糖
- 主营：丙酸钙、肉桂酸、维生素C/抗坏血酸、琼脂糖、edta二钠、纳他霉素、抗氧剂264、抗坏血酸、双乙酸钠、聚赖氨酸、胍基乙酸、抗氧剂t501、抗氧剂1010、抗氧剂dltp、半胱氨酸盐酸盐、半胱氨酸、果胶、干酪素、苯丙氨酸、乳酸链球菌素、肉桂酸钾、色氨酸、牛磺酸、低聚木糖、酪蛋白酸钠、维生素C、vc
- 主营：蛋白酶、淀粉酶、果胶酶、琼脂糖、胰酵素、崩溃酶、蜗牛酶、乳糖酶、肌酸激酶、葡球菌酶、绿色木霉、心肌黄酶、亮抑酶肽、纤维素酶、木聚糖酶、-glucosidase、亮抑肽酶、硫代辅酶、枯草杆菌、羧化辅酶、氯化酵素、脆壁质酶、细胞溶解酶、乙醇脱氢酶、透明质酸酶、氯化溶菌酶
- 主营：琼脂糖、标准品、DRE
- 主营：高铁酸钾
- 主营：6-二羟基吲哚、磷钼酸、艾拉莫德中间体、琼脂糖、甲基丙二酸、光引发剂、苯芴醇、溴芬酸钠中间体、樟脑醌、钼酸钾、丝素蛋白、盐酸胍、β-丙氨酸甲酯盐酸盐、聚二羟基吲哚、7-去氢胆固醇、甘氨胆酸钠盐、半叶素、胆固醇、2-辛基十二醇、蛋白酶K、D-荧光素钾盐、光引发剂LAP、BIS-TRIS、甲基丙烯酸氧杂环丁烷
- 主营：架桥剂、YW100 锂萃取剂、端羧基液体丁腈橡胶、琼脂糖30210、10-羟基癸酸、苯乙烯磷酸、肉豆蔻酸镁、硬脂酸钴、全氟三丁胺、乙烯基磷酸二乙酯、磷酸锰铁锂、β-磷酸三钙、十一烯酸、钨酸铝、1-乙炔基环已醇、间二异丙苯、锂硅合金、溴甲酚绿、生物活性玻璃、溴代海因树脂颗粒、红铝、龙涎酮、原丁酸三甲酯
- 主营：有机原料、中间体、香精香料、琼脂糖、催化剂、有机硅、表面活性剂
- 主营：全氟聚醚二醇、全氟三丁胺、聚硅氮烷、低电渗琼脂糖、特种硅油、特种硅树脂
- 主营：10ul吸头、巴氏吸管、离心管、琼脂糖、透析袋、国产试剂
- 主营：琼脂糖、全氟三丁胺、木质素、吉氏色素