Overview
Golgi phosphoprotein is a key component of the Golgi apparatus, primarily involved in maintaining the structure and function of this essential cellular organelle. It plays significant roles in protein glycosylation, sorting, and vesicular transport processes. The protein is characterized by multiple phosphorylation sites that regulate its interaction with other cellular components. First identified in the late 1990s, Golgi phosphoprotein has since been recognized as crucial for proper Golgi stacking and ribbon formation. Its expression levels and phosphorylation status have been linked to various cellular processes, making it an important subject of study in cell biology and disease research.
Physical and Chemical Properties
Golgi phosphoprotein typically presents as a soluble protein with a molecular weight ranging between 50-60 kDa, depending on isoform and post-translational modifications. The protein contains numerous phosphorylation sites that can alter its charge and binding properties under different cellular conditions. In solution, the protein is relatively stable when stored properly at cold temperatures. Its solubility in aqueous buffers makes it suitable for various biochemical applications. The phosphoprotein nature makes it particularly interesting for studies involving protein-protein interactions and signal transduction pathways related to Golgi function.
Main Applications
In research settings, Golgi phosphoprotein is primarily used to study Golgi apparatus dynamics and membrane trafficking mechanisms. Scientists employ it to investigate how phosphorylation affects protein localization and organelle morphology. The protein has become particularly relevant in cancer research, as alterations in Golgi structure are observed in many malignant cells. Additionally, this phosphoprotein serves as a valuable tool for developing assays to screen potential drugs targeting Golgi-related pathways. Some studies suggest its potential as a biomarker for certain diseases, though clinical applications remain in early stages of investigation.
Safety and Storage
While Golgi phosphoprotein is generally considered non-toxic, standard laboratory precautions should be observed when handling. This includes wearing appropriate personal protective equipment and working in controlled environments to prevent contamination. For optimal preservation, the protein should be stored at -20°C or preferably -80°C in small aliquots to avoid repeated freeze-thaw cycles that could degrade its quality. Lyophilized preparations typically have longer shelf lives when stored desiccated at low temperatures. Always verify storage recommendations from the specific supplier.
B2B Procurement Guide
When sourcing Golgi phosphoprotein for research or industrial applications, prioritize suppliers with demonstrated expertise in protein production and characterization. Key procurement considerations include verifying certificate of analysis data for purity (typically >95% by SDS-PAGE), confirming biological activity through appropriate assays, and ensuring proper documentation of post-translational modification status. For consistent results, consider establishing long-term relationships with reputable suppliers who can provide technical support and batch-to-batch consistency. Bulk purchases may offer cost advantages for large-scale projects, but always validate new batches before full implementation in critical applications.
Related Manufacturers
- 主营：ELISA试剂盒、生化检测试剂盒、PCR试剂盒、大鼠ELISA试剂盒、小鼠ELISA试剂盒、兔ELISA试剂盒、鸡ELISA试剂盒
- 主营：试剂盒、酶联免疫检测试剂盒、检测试剂盒、人锚定蛋白、免疫球蛋白、磷脂酶域蛋白、寡聚化域蛋白、抗突触核蛋白、人层粘连蛋白、易位断点蛋白、人特异性蛋白、病毒样核蛋白、诱导反应蛋白、突触关联蛋白、人动力蛋白胞浆、人突触囊泡蛋白、溶酶体辅助蛋白、人血管抑制蛋白、人醛脱氢酶、人乙醇脱氢酶、前列腺素还原酶、磷脂酰肌醇聚糖
- 主营：ELISA试剂盒、检测试剂盒、生化试剂盒、pcr检测试剂盒、细胞
- 主营：ELISA检测试剂盒、抗体、ELISA试剂盒
- 主营：瘤细胞、细胞株、肉毒碱、脂蛋白、蛋白酶、切蛋白、样蛋白、信号素、测试盒、癌细胞、羧化酶、子宫肌、质激素、羧肽酶、坏血酸、合成酶、视网膜、同源物、骨钙素、羧激酶、白介素、加压素、纤溶酶、骨桥素、干扰素
- 主营：骨细胞、腺嘌呤、微量法、脂蛋白、粘蛋白、蛋白酶、蛋白质、试剂盒、提试剂、增菌液、测试盒、光度法、瘤细胞、纤维素、信号素、缺锌ynb、病毒探、肝细胞、饥饿素、外泌体、混合液、半乳糖、抑制剂、激活肽、癌细胞
- 主营：咽鳞癌细胞、结肠癌细胞、急性淋巴细胞、GOLPH2活性蛋白、永生化表皮细胞