Overview
Fluorescent staining reagents are specialized chemicals designed to bind selectively to biological molecules (e.g., DNA, proteins) or cellular structures, emitting detectable light upon excitation. They revolutionized life sciences by enabling real-time visualization of dynamic processes in cells and tissues. Common classes include nucleic acid stains (e.g., SYBR Green), antibody-conjugated fluorophores (e.g., Alexa Fluor), and organelle trackers (e.g., MitoTracker). These reagents are indispensable in modern research, clinical diagnostics, and drug discovery. Their development aligns with advancements in fluorescence microscopy and spectral imaging technologies, offering increasingly precise and multiplexed detection capabilities.
Physical and Chemical Properties
Fluorescent reagents exhibit distinct spectral properties, including excitation/emission maxima (e.g., DAPI: 358/461 nm) and Stokes shifts. Many are aromatic compounds with conjugated double bonds, granting photostability and tunable wavelengths. Their solubility varies: hydrophilic dyes (e.g., FITC) suit aqueous solutions, while lipophilic dyes (e.g., DiO) label membranes. Photobleaching resistance is critical; newer reagents like CF dyes minimize signal decay. Some require covalent bonding (e.g., NHS esters for protein labeling), while others intercalate non-covalently (e.g., ethidium bromide with DNA). Storage conditions often involve refrigeration and desiccation to preserve reactivity.
Main Applications
In research, these reagents enable live-cell imaging, co-localization studies, and FRET-based interaction analyses. DAPI stains nuclei in fixed cells, while Calcein-AM assesses viability. Clinically, they underpin FISH (fluorescence in situ hybridization) for genetic abnormalities and immunofluorescence assays for autoimmune diseases. Flow cytometry relies on multiplexed fluorophores (e.g., PE-Cy7 conjugates) for immunophenotyping. Emerging uses include super-resolution microscopy (e.g., PALM/STORM) and in vivo imaging with near-infrared probes. Industrial applications span quality control in biomanufacturing and environmental monitoring of microorganisms.
Safety and Storage
Many fluorescent dyes are mutagenic (e.g., ethidium bromide) or cytotoxic (e.g., propidium iodide). Always consult SDS and use fume hoods for powders. Dispose of waste via approved hazardous channels, as some reagents persist in ecosystems. Store lyophilized dyes in airtight containers with desiccants; reconstituted solutions often require aliquoting to freeze-thaw cycles. Light-sensitive dyes (e.g., Cyanine derivatives) need amber vials. Label containers clearly with excitation/emission data to avoid cross-contamination during experiments.
B2B Procurement Guide
Prioritize suppliers with ISO 13485 certification for diagnostic-grade reagents or GMP compliance for clinical use. Bulk buyers should request batch-specific QC reports (HPLC purity, fluorescence validation). Consider conjugation services for custom antibody-fluorophore pairs. For high-throughput screening, opt for plates pre-coated with detection dyes. Evaluate alternatives like polymer-based dyes (e.g., Brilliant Violet) for brighter signals. MOQs typically start at 1g, with discounts for multi-gram orders. Lead times vary; specialty probes may require 4-6 weeks for synthesis.
Related Manufacturers
- 主营：甜菊糖、氯乙酰、二乙苯、酞菁绿、阻燃剂、4316-73-8、二戊烯、单甲醚、硫酸锂、4861-19-2、大理石、1192-62-7、羊脂酸、7783-40-6、磷酸氢、8002-74-2、7782-61-8、龙脑油、2842-44-6、聚甘油、二聚酸、戊二醇、聚醚f68、磺化油、1310-58-3
- 主营：硫酸钡、覆盆子酮、胍基丙酸、荧光素钠、早强防冻剂、食品级磷酸镁、二肌酸苹果酸、苯基膦酰二氯、食品级硫酸镁、二肌酸柠檬酸
- 主营：环戊酮、甲酸镍、阻燃剂、荧光素钠、草酸镧、钼酸铵、催化剂、钒酸铋、溴氨酸、硝酸锆、碳酸镧、白僵菌、磷酸铁、钨酸酐、氟化镧、视黄醇、叔丁醇、杀螨剂、杀虫剂、醋酸钬、抗粘剂、氟化镥、肟菌酯、氢化钠、氯铂酸、氟化镁
- 主营：增塑剂、草酸镍、阻燃剂、荧光素、栀子蓝、抗氧剂、偶联剂、异壬酸、1-二十烯、间氟溴苄、异胞嘧啶、磷酸三钾、油墨油剂、洗涤助剂、苯磺酸钠、单体香料、环磺酮酸、分散染料、无机颜料、苹果酸镁、表面处理剂、丙二醇甲醚、化妆品原料、丁酸甘油酯、硅烷处理剂、表面活性剂
- 主营：测定仪、粉尘测定、检测系统、真菌荧光染色液、前处理工作、处理工作站、快速分析仪、元素分析仪、滴虫检测仪、九项检测仪器、碘元素检测仪
- 主营：细胞凋亡、蛋白提取、细胞增殖毒性检测、试剂盒、核蛋白提取、膜蛋白提取、外泌体提取、细胞器分离、细胞周期检测、细胞焦亡检测、植物蛋白提取
- 主营：耗材、仪器、实验耗材、试剂、转染试剂、takara试剂、qiagen试剂盒、zymo试剂盒、goldbio荧光素、EZB试剂盒、细胞核悬液制备试剂盒、解离试剂盒、origene蛋白、CST抗体、abcam抗体、permagen磁力、polyplus转染、AcceGen细胞、Toxin肠毒素、速溶颗粒、DMEM培养基、胎牛血清、alzet渗透泵、伊莱瑞特、millipore
- 主营：elisa试剂盒、生物试剂、荧光试剂盒、化学试剂、比色法试剂盒、抗体、培养基、标准品
- 主营：分析仪、水碘检测、尿碘检测仪、检测试剂盒、荧光染色液、测定试剂盒、盐碘试剂盒、真菌荧光、采样机器人、体质测试仪、体质辨识仪、元素检测仪、尿碘测定仪、分光光度计、分子筛制氧机、分泌物检测仪、原子吸收分光、微量元素、碘元素、阴道分泌物、白带分析仪、盐碘、水碘、尿碘、二氧化硅
- 主营：微量元素检测仪、阴道分泌物分析仪、骨密度检测仪、真菌荧光染色液、人体成分分析仪、身高体重测量仪、儿童综合素质测试仪、儿童膳食营养分析仪、母乳分析仪、儿童认知能力测评、便携式骨密度仪、儿童智力测试仪、阴道微生态检测仪、中医体质辨识仪、中医四诊仪、中医舌诊仪、中医脉诊仪、听觉统合训练仪、语言障碍诊治仪、构音评估与训练仪、言语矫治训练仪器、儿童认知能力测试训练、卧式婴幼儿体重秤、视听统合训练仪
- 主营：cy荧光染料、ICG吲哚菁绿、源头厂家
- 主营：检测试剂盒
- 主营：阻燃剂、催化剂、洗涤剂、生物试剂、硫酸铵、醋酸镧、起泡剂、清洗剂、碳酸钙、抗氧剂、灭火剂、交联剂、粘合剂、封头剂、丙二醇、酸二氢铝、聚乙烯醇、对氯溴苯、息香乙醚、异戊二醇、香精香料、4-丁二醇、2-氯丙烯腈、2-己基癸酸、二甲胺硼烷
- 主营：微量元素分析仪、儿童身高体重测量仪、阴道分泌物分析仪、真菌荧光染色液、骨密度检测仪、中医体质辨识仪、听觉统合训练仪、母乳分析仪、中医四诊仪、儿童智力测试仪、儿童综合素质测试仪、儿童注意力测试仪、人体成分分析仪、膳食营养分析仪、语言障碍诊治仪、构音评估与训练仪、言语矫治训练仪、中医经络检测仪、中医舌诊仪、中医脉诊仪、儿童认知能力测试训练、便携式骨密度仪、中医健康宝、健康体检一体机、全自动微量元素检测仪
- 主营：生物dna、dna病毒、dna回收、小提试剂盒、提取试剂盒、植物dna、dna产物、基因组dna、两步法细菌、离心柱式血液、酵母质粒提取、蛋白浓度测定、细胞增殖检测