Overview
Ergosterol Fluorescein is a synthetic sterol derivative where ergosterol, a fungal sterol structurally similar to cholesterol, is conjugated with fluorescein. This hybrid molecule is designed for fluorescence microscopy and biochemical assays, enabling real-time visualization of sterol distribution and dynamics in cellular membranes. The compound is particularly useful in studying lipid rafts, membrane fluidity, and interactions between sterols and proteins. Its applications span microbiology, pharmacology, and cell biology, providing insights into processes like fungal membrane organization and drug interactions.
Physical and Chemical Properties
Ergosterol Fluorescein exhibits bright green fluorescence when excited at ~494 nm, with emission peaks around ~521 nm. Its photostability and solubility in organic solvents like DMSO make it suitable for labeling membranes without disrupting native lipid bilayers. The chemical stability of the conjugate depends on storage conditions; degradation may occur under prolonged light exposure or at room temperature. Analytical techniques such as HPLC and mass spectrometry are often used to verify purity and batch consistency.
Main Applications
In research, Ergosterol Fluorescein is primarily employed to track sterol movement in live cells, especially in fungi like yeast. It helps elucidate mechanisms of antifungal drug action by visualizing sterol redistribution during treatment. Other uses include studying lipid-protein interactions in model membranes and developing fluorescence-based assays for high-throughput screening of lipid-modulating compounds. Its compatibility with confocal and super-resolution microscopy enhances spatial resolution in dynamic studies.
Safety and Storage
As a laboratory reagent, Ergosterol Fluorescein requires handling with gloves and eye protection due to potential irritant effects. It should be stored at -20°C in airtight, light-proof containers to prevent photobleaching and chemical degradation. Disposal must follow institutional guidelines for organic fluorescent compounds. Avoid aqueous solutions unless stabilized with surfactants, as precipitation may occur.
B2B Procurement Guide
When sourcing Ergosterol Fluorescein, prioritize suppliers specializing in fluorescent probes or lipid analogs. Key procurement considerations include batch-specific certificates of analysis (CoA), which detail purity (>95% preferred) and solvent compatibility. For bulk orders, negotiate pricing based on volume and request custom conjugation services if specific fluorescein derivatives (e.g., FITC alternatives) are needed. Lead times may vary due to synthetic complexity.
Related Manufacturers
- 主营：DMG-PEG
- 主营：架桥剂、YW100 锂萃取剂、端羧基液体丁腈橡胶、麦角甾醇、10-羟基癸酸、苯乙烯磷酸、肉豆蔻酸镁、硬脂酸钴、全氟三丁胺、乙烯基磷酸二乙酯、磷酸锰铁锂、β-磷酸三钙、十一烯酸、钨酸铝、1-乙炔基环已醇、间二异丙苯、锂硅合金、溴甲酚绿、生物活性玻璃、溴代海因树脂颗粒、红铝、龙涎酮、原丁酸三甲酯
- 主营：麦角甾醇、对照品、标准品
- 主营：苦参碱、氧化苦参碱、七叶皂苷钠、麦角甾醇、七叶皂苷、黑米花色苷、鬼臼树脂、鬼臼毒素、黑米花青素、龙胆苦苷、青蒿琥酯、青蒿素、吴茱萸碱、吴茱萸次碱、博落回总碱、鱼藤酮、二苯乙烯苷、丝肽粉、人参皂甙
- 主营：石油醚、四氢噻吩、顺酐、苯酐
- 主营：10ul吸头、巴氏吸管、离心管、麦角甾醇、透析袋、琼脂糖、国产试剂
- 主营：中药标准品、标准品对照品、天然化合物、麦角甾醇、标准品、分析试剂、细胞实验、动物实验、中药提取物、单体化合物、科研实验用
- 主营：精萃宝对照品、一叶萩碱、三叶豆紫檀苷、羟基红花黄色素A、苍术素、苍术酮、盐酸血根碱、盐酸白屈菜红碱、药根碱、藁本内酯、欧当归内酯A、高丽槐素、松果菊苷、马钱苷、莫诺苷
- 主营：麦角甾醇试剂盒、elisa试剂盒、试剂盒、抗体
- 主营：麦角甾醇、苄基三乙基氯化铵、苄基三甲基氯化铵
- 主营：植物醇、植物提取物、8-二氨基萘、4-萘二甲酸、8-氨基喹哪啶、聚二羟基吲哚、2-巯基噻唑啉、6-二羟基吲哚氢、磷钼酸、石胆酸、乙烯基膦酸、劳伦酸、二水合钼酸钠、4-辛氧基二苯碘六氟、麦角硫因、DL-樟脑醌、羟乙基磺酸、钼酸、甲基丙烯酸氧杂环丁烷、三羟甲基氨基甲烷盐酸、4-羟乙基哌嗪乙磺酸、5-甲基 N-乙烯基、腺苷、L-谷氨酸-5-叔丁、聚季铵盐-55
- 主营：Elisa试剂盒、生化试剂盒、蛋白试剂盒、羟麦角甾醇检测、细胞试剂盒
- 主营：ELISA试剂盒、细胞培养系列、PCR相关、麦角甾醇、透析袋系列、实验耗材系列、实验室试剂系列
- 主营：对照品、标准品、三叶苷、麦角甾醇、姜烯酚、人参皂苷、地黄苷、科研试剂、实验室自制、高纯度植物单体、生育三烯酚
- 主营：甲基原、钩藤碱、络塞定、丹皮酚、2086-83-1、6836-11-9、鞣花酸、川楝素、黄芩素、苍术素、松脂素、管花苷、熊果酸、苦参碱、6926-08-5、土莫酸、苜蓿素、茯苓酸、柚皮素、3668-14-2、白麻苷、秦皮苷、葛花苷、山奈酚、柚皮苷