Fluorescein[2]
Overview
Fluorescein is a xanthene dye first synthesized in 1871 by Adolf von Baeyer. It is renowned for its intense fluorescence, emitting bright green-yellow light when exposed to blue or UV light. This property makes it invaluable in fields requiring high-visibility tracing, such as medical diagnostics and environmental studies. As a pH-sensitive compound, fluorescein exhibits stronger fluorescence in alkaline conditions. Its sodium salt (fluorescein sodium) is water-soluble and commonly used in ophthalmic angiography to visualize blood flow in the retina. The compound is also a precursor to derivatives like fluorescein isothiocyanate (FITC), a staple in antibody labeling.
Physical and Chemical Properties
Fluorescein is an orange-red crystalline powder with a molecular weight of 332.31 g/mol. It decomposes at 314–316°C without a distinct boiling point. The dye’s fluorescence peaks at 512 nm when dissolved in water, with excitation maxima at 494 nm (blue light). Its solubility varies: fluorescein sodium dissolves readily in water, while the free acid form requires alkaline conditions. The compound is stable under dry, dark storage but degrades under prolonged UV exposure. Its fluorescence intensity is highly pH-dependent, diminishing in acidic environments (pH < 5).
Main Applications
In ophthalmology, fluorescein is used in fluorescein angiography to diagnose retinal disorders by highlighting blood vessel leakage. It’s also applied in contact lens fitting and corneal injury detection. Industrially, the dye serves as a tracer in hydrogeology to map water flow and detect leaks in pipelines or reservoirs. In biochemistry, FITC-labeled antibodies enable fluorescence microscopy. Additionally, fluorescein aids in environmental monitoring, such as tracking pollutant dispersion in waterways.
Safety and Storage
Fluorescein is generally low-toxicity but may irritate eyes, skin, or respiratory tract. Use gloves and goggles when handling powdered forms. Avoid inhalation and ensure adequate ventilation. Store in airtight containers away from light and moisture to prevent degradation. Bulk quantities should be kept in temperature-controlled environments (15–25°C). Disposal must comply with local regulations due to its potential environmental persistence.
B2B Procurement Guide
For medical-grade fluorescein, prioritize suppliers compliant with USP/EP/JP pharmacopeia standards. Industrial users should specify purity (≥90% for non-medical uses) and request SDS documentation. Bulk buyers should negotiate pricing for orders exceeding 50 kg, with discounts commonly available. Verify supplier batch testing reports for consistency in fluorescence yield and impurity profiles. Lead times vary; ensure alignment with project timelines.
Related Manufacturers
- 主营：反应活、hydrazide、修饰剂、催化剂、糖蛋白、连接子、激动剂、聚合物、活性脂、胆固醇、钆特酸、催化性、dna标记、络合剂、荧光试剂、雨蛙素、乙二胺、ped结构、衍生物、化合物、链接剂、反应物、生物素
- 主营：加帽酶、激活剂、甘氨酸、igg抗体、稳定液、玻璃板、试剂盒、脂蛋白、超纯水、激动剂、胆固醇、盐酸盐、褪黑素、因质粒、扩容剂、组化笔、驴抗人、预制胶、驴抗兔、生物驴、草甘膦、重组酶、生物素、曲拉通、去除剂
- 主营：混匀仪、缓冲液、宿主鼠、冻存袋、微孔板、基质胶、裂解液、培养基、宿主兔、抗抗体、分子量、柱磁珠、培养板、参抗体、移液器、浅孔板、离心机、分选柱、试剂盒、商品chir、流式抗体、试剂产品、二抗试剂、筛网滤器、免疫细胞
- 主营：检测试剂盒、Elisa试剂盒、农残检测试剂盒、试剂、液相检测、测试盒、检测卡、比色法检测、荧光法检测
- 主营：金纳米、三角片、麦芽糖、羟丙基、ito薄膜、氮化钛、tio2纳米、碳粉tio2、修饰tio2、pd纳米笼、水溶伊红、纳米复合、磁性纳米、蛋白芯片、包硅金银、硅纳米线、包硅纳米、硅纳米管、螯合树脂、油溶性银、复合材料、薄膜定制、纳米粒子
- 主营：嵌段共聚物、合成磷脂、荧光染料、PEG、点击化学、量子点
- 主营：胆固醇荧光探针、罗丹明B酰肼、深红溶酶体荧光探针、缺氧探针、鬼笔环肽、钙黄绿素红、钙螯合剂、葡萄糖摄取荧光探针、红色荧光标记鬼笔环肽、深红色溶酶体荧光探针、SPQ氯离子荧光探针、钙离子荧光探针、Permeant、脂膜荧光探针、C-Laurdan、SPQ、LysoTracke、stain670、6-NBDG、荧光探针、葡萄糖荧光探针、酶体荧光探针、红色荧光鬼笔环肽、深红色荧光探针
- 主营：在水中、包含肽、含叠氮、丙炔基、由氨基、菁染料、peg单元、具有炔、胺hcl盐、阿尔法、催化剂、peg试剂、结构式、氨基nh2、3694-51-7、碘化物、4727-50-8、具有两、带有炔、磺酸基、3065-79-0、aminopeg8、甲基九、bcn基团、荧光素
- 主营：4-二甲氨基吡啶、N-乙烯基己内酰胺、2-氨基噻唑、苯甲酸酐、4-乙烯二氧噻吩、1-羟基苯并三唑无水、草酸氧钒、对羟基苯乙胺、对二甲氨基苯甲醛、六氟磷酸铵、烯丙基硫脲
- 主营：多肽合成、多肽定制、磷酸化修饰、抑制剂与底物肽、药物肽、标签肽、淀粉样多肽、棕榈酰三肽、乙酰基六肽、蛇毒肽
- 主营：生物素修饰蛋白、fitc标记蛋白、聚苯乙烯微球、pvc微球、嵌段共聚物、生物素标记、荧光标记、纳米颗粒、核苷糖、aie、复合微球、功能化载玻片、荧光染料、金属配合物、Mxene、介孔二氧化硅微球、磁性纳米颗粒、小分子PEG、聚合物、纳米金、聚合物微球、荧光微球、荧光探针、点击化学、小分子
- 主营：6-二羟基吲哚、二羟基吲哚啉氢溴酸、水解角蛋白、7-去氢胆固醇、石胆酸钠盐、熊去氧胆酸钠盐、2-辛基十二醇、2-巯基噻唑啉、2-巯基苯并咪唑、苯基周位酸(8-苯胺、L-丙交酯、半叶素、乙烯利、环丙磺酰胺、苄氧基胺盐酸盐、1-氨基环丙烷羧酸、4-联苯甲醇丙烯酸酯、4-羟乙烯基氧基二苯、咪鲜胺、可比落、乙交酯、D(+)-丙交酯
- 主营：Difluorofluorescein、白蜡树素、百两金素、半齿泽兰素
- 主营：甲氧基聚乙二醇、氨基聚乙二醇、羧基聚乙二醇、巯基聚乙二醇、羟基聚乙二醇、马来酰亚胺聚乙二醇、四臂聚乙二醇、荧光素聚乙二醇、叠氮聚乙二醇、硅烷聚乙二醇、炔基聚乙二醇、生物素聚乙二醇、丙烯酸酯聚乙二醇、活性脂聚乙二醇、Boc保护基团聚乙二醇、Fmoc保护基团聚乙二醇、生物素小分子PEG衍生物、甲氧基小分子PEG衍生物
- 主营：生物活性玻璃、烟酰胺、王浆酸、赖氨酸、锆氟酸、氢化镁